Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species
Two types of materials, six compositions of extractions buffer and three electrophoretic buffer system were studied to establish optimum procedures for obtaining good resolution of 14 enzyme systems in 12 species of Shorea which belongs to the family of Dipterocarpaceace. Thirteen (13) enzyme system...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1997
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/17898 |
| _version_ | 1855531029999124480 |
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| author | Sudarmonowati, E. Hartarti, N.S. Siregar, U.J. |
| author_browse | Hartarti, N.S. Siregar, U.J. Sudarmonowati, E. |
| author_facet | Sudarmonowati, E. Hartarti, N.S. Siregar, U.J. |
| author_sort | Sudarmonowati, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two types of materials, six compositions of extractions buffer and three electrophoretic buffer system were studied to establish optimum procedures for obtaining good resolution of 14 enzyme systems in 12 species of Shorea which belongs to the family of Dipterocarpaceace. Thirteen (13) enzyme systems could be detected in S.seminis and S.parvifolia, 10 of that in S. laevis, S.leprosula, S.selanica, S.aquminata, S.gibbosa, S.palembanica, and S.pauciflora, 7 of that in S.paquetiana and 4 of that in S.johorensis. The use of W extraction buffer in combination with the use of MC electrophoretic buffer systems led to the best results of using twigs were almost the same as leaves, better resolution of certain enzyme system such as PER, PGI, SDH, and MDH was obtained from leaves. Results obtained from this study would benefit further study on various aspects such as genetic diversity assessment of Shorea species and genetic improvement. |
| format | Journal Article |
| id | CGSpace17898 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| record_format | dspace |
| spelling | CGSpace178982025-01-24T14:19:55Z Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species Sudarmonowati, E. Hartarti, N.S. Siregar, U.J. shorea forest trees genetic markers species Two types of materials, six compositions of extractions buffer and three electrophoretic buffer system were studied to establish optimum procedures for obtaining good resolution of 14 enzyme systems in 12 species of Shorea which belongs to the family of Dipterocarpaceace. Thirteen (13) enzyme systems could be detected in S.seminis and S.parvifolia, 10 of that in S. laevis, S.leprosula, S.selanica, S.aquminata, S.gibbosa, S.palembanica, and S.pauciflora, 7 of that in S.paquetiana and 4 of that in S.johorensis. The use of W extraction buffer in combination with the use of MC electrophoretic buffer systems led to the best results of using twigs were almost the same as leaves, better resolution of certain enzyme system such as PER, PGI, SDH, and MDH was obtained from leaves. Results obtained from this study would benefit further study on various aspects such as genetic diversity assessment of Shorea species and genetic improvement. 1997 2012-06-04T09:04:43Z 2012-06-04T09:04:43Z Journal Article https://hdl.handle.net/10568/17898 en Sudarmonowati, E., Hartarti, N.S., Siregar, U.J. 1997. Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species . Annales Bogorienses 5 (1) :1-14. |
| spellingShingle | shorea forest trees genetic markers species Sudarmonowati, E. Hartarti, N.S. Siregar, U.J. Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species |
| title | Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species |
| title_full | Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species |
| title_fullStr | Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species |
| title_full_unstemmed | Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species |
| title_short | Establishment of biochemical genetic markers techniques for Shorea spp.- tropical forest tree species |
| title_sort | establishment of biochemical genetic markers techniques for shorea spp tropical forest tree species |
| topic | shorea forest trees genetic markers species |
| url | https://hdl.handle.net/10568/17898 |
| work_keys_str_mv | AT sudarmonowatie establishmentofbiochemicalgeneticmarkerstechniquesforshoreaspptropicalforesttreespecies AT hartartins establishmentofbiochemicalgeneticmarkerstechniquesforshoreaspptropicalforesttreespecies AT siregaruj establishmentofbiochemicalgeneticmarkerstechniquesforshoreaspptropicalforesttreespecies |