Genome editing in banana: tools, techniques, and challenges

Bananas (Musa spp.) are one of the most important staple crops and fruit sources, providing livelihoods for millions of farmers in tropical and subtropical regions worldwide. It is cultivated in over 145 countries and small island nations, crucial to food security. However, banana production faces s...

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Main Authors: Tripathi, J., Kariuki, S.M., Waweru, D.N., Tripathi, L.
Format: Book Chapter
Language:Inglés
Published: Springer 2025
Subjects:
Online Access:https://hdl.handle.net/10568/178464
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author Tripathi, J.
Kariuki, S.M.
Waweru, D.N.
Tripathi, L.
author_browse Kariuki, S.M.
Tripathi, J.
Tripathi, L.
Waweru, D.N.
author_facet Tripathi, J.
Kariuki, S.M.
Waweru, D.N.
Tripathi, L.
author_sort Tripathi, J.
collection Repository of Agricultural Research Outputs (CGSpace)
description Bananas (Musa spp.) are one of the most important staple crops and fruit sources, providing livelihoods for millions of farmers in tropical and subtropical regions worldwide. It is cultivated in over 145 countries and small island nations, crucial to food security. However, banana production faces significant challenges due to various biotic and abiotic factors. The bananas’ narrow genetic diversity and long generation cycle make conventional breeding methods difficult and time-consuming. Genome editing offers a powerful approach to developing high-yield, disease-resistant banana varieties. Several genome editing technologies enable precise and targeted modifications within the plant genome. Among these, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based tools stand out for their significant advantages, though they also present regulatory and technical challenges. In this chapter, we describe the importance of banana cultivation, examine the benefits and limitations of various CRISPR tools, explore advancements in banana genome editing, and cover the technical and regulatory aspects of implementing genome editing for banana improvement.
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spelling CGSpace1784642025-12-03T01:24:13Z Genome editing in banana: tools, techniques, and challenges Tripathi, J. Kariuki, S.M. Waweru, D.N. Tripathi, L. bananas gene editing musa Bananas (Musa spp.) are one of the most important staple crops and fruit sources, providing livelihoods for millions of farmers in tropical and subtropical regions worldwide. It is cultivated in over 145 countries and small island nations, crucial to food security. However, banana production faces significant challenges due to various biotic and abiotic factors. The bananas’ narrow genetic diversity and long generation cycle make conventional breeding methods difficult and time-consuming. Genome editing offers a powerful approach to developing high-yield, disease-resistant banana varieties. Several genome editing technologies enable precise and targeted modifications within the plant genome. Among these, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based tools stand out for their significant advantages, though they also present regulatory and technical challenges. In this chapter, we describe the importance of banana cultivation, examine the benefits and limitations of various CRISPR tools, explore advancements in banana genome editing, and cover the technical and regulatory aspects of implementing genome editing for banana improvement. 2025-10-01 2025-12-03T01:24:12Z 2025-12-03T01:24:12Z Book Chapter https://hdl.handle.net/10568/178464 en Limited Access Springer Tripathi, J.N., Kariuki, S.M., Waweru, D.N. & Tripathi, L. (2025). Genome editing in banana: tools, techniques, and challenges. In Y. Ahlawat, D. Chaudhary, P. Jaiwal and P.K. Jaiwal, Next-generation strategies for crop improvement. Singapore: Springer Nature Singapore, (p. 51-79).
spellingShingle bananas
gene editing
musa
Tripathi, J.
Kariuki, S.M.
Waweru, D.N.
Tripathi, L.
Genome editing in banana: tools, techniques, and challenges
title Genome editing in banana: tools, techniques, and challenges
title_full Genome editing in banana: tools, techniques, and challenges
title_fullStr Genome editing in banana: tools, techniques, and challenges
title_full_unstemmed Genome editing in banana: tools, techniques, and challenges
title_short Genome editing in banana: tools, techniques, and challenges
title_sort genome editing in banana tools techniques and challenges
topic bananas
gene editing
musa
url https://hdl.handle.net/10568/178464
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