Agrobacterium tumefaciens–mediated genome editing in banana
This protocol outlines the Agrobacterium tumefaciens–mediated transformation process for genome editing in banana (Musa spp.). As a crop of significant agricultural and economic importance globally, improving banana cultivars is crucial for addressing challenges such as disease resistance, climate r...
| Main Authors: | , |
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| Format: | Book Chapter |
| Language: | Inglés |
| Published: |
Humana Press
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/178440 |
| _version_ | 1855541955237249024 |
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| author | Tripathi, J. Tripathi, L. |
| author_browse | Tripathi, J. Tripathi, L. |
| author_facet | Tripathi, J. Tripathi, L. |
| author_sort | Tripathi, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This protocol outlines the Agrobacterium tumefaciens–mediated transformation process for genome editing in banana (Musa spp.). As a crop of significant agricultural and economic importance globally, improving banana cultivars is crucial for addressing challenges such as disease resistance, climate resilience and yield enhancement. The procedure presented here involves the establishment of embryogenic cell suspensions (ECSs) from immature male flowers, followed by co-cultivation with Agrobacterium containing the desired gene construct. Key steps include callus induction, ECS generation and maintenance, transformation using plasmid construct containing genome editing reagents such as CRISPR/Cas9, and regeneration. The method ensures the editing of target genes in the banana genome, facilitating genetic improvements. |
| format | Book Chapter |
| id | CGSpace178440 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Humana Press |
| publisherStr | Humana Press |
| record_format | dspace |
| spelling | CGSpace1784402025-12-08T10:29:22Z Agrobacterium tumefaciens–mediated genome editing in banana Tripathi, J. Tripathi, L. bananas musa flowers agrobacterium tumefaciens gene editing This protocol outlines the Agrobacterium tumefaciens–mediated transformation process for genome editing in banana (Musa spp.). As a crop of significant agricultural and economic importance globally, improving banana cultivars is crucial for addressing challenges such as disease resistance, climate resilience and yield enhancement. The procedure presented here involves the establishment of embryogenic cell suspensions (ECSs) from immature male flowers, followed by co-cultivation with Agrobacterium containing the desired gene construct. Key steps include callus induction, ECS generation and maintenance, transformation using plasmid construct containing genome editing reagents such as CRISPR/Cas9, and regeneration. The method ensures the editing of target genes in the banana genome, facilitating genetic improvements. 2025 2025-12-02T13:05:39Z 2025-12-02T13:05:39Z Book Chapter https://hdl.handle.net/10568/178440 en Limited Access Humana Press Tripathi, J.N. & Tripathi, L. (2025). Agrobacterium tumefaciens–mediated genome editing in banana. In C. Klein (eds) Agrobacterium. Methods in molecular biology, vol 2911. New York, USA: Humana, (p. 143-153). |
| spellingShingle | bananas musa flowers agrobacterium tumefaciens gene editing Tripathi, J. Tripathi, L. Agrobacterium tumefaciens–mediated genome editing in banana |
| title | Agrobacterium tumefaciens–mediated genome editing in banana |
| title_full | Agrobacterium tumefaciens–mediated genome editing in banana |
| title_fullStr | Agrobacterium tumefaciens–mediated genome editing in banana |
| title_full_unstemmed | Agrobacterium tumefaciens–mediated genome editing in banana |
| title_short | Agrobacterium tumefaciens–mediated genome editing in banana |
| title_sort | agrobacterium tumefaciens mediated genome editing in banana |
| topic | bananas musa flowers agrobacterium tumefaciens gene editing |
| url | https://hdl.handle.net/10568/178440 |
| work_keys_str_mv | AT tripathij agrobacteriumtumefaciensmediatedgenomeeditinginbanana AT tripathil agrobacteriumtumefaciensmediatedgenomeeditinginbanana |