Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers
Sweet corn (Zea mays L. saccharata) is an important specialty maize, valued for its sweetness and nutritional quality with high consumer demand. The development of superior hybrids relies on genetically diverse inbred lines. Advances in genotyping technologies have transformed the way breeding progr...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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ScienceDomain International
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/178397 |
| _version_ | 1855525189301829632 |
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| author | Somanatha Patil, Ayyanagouda Kuchanur, Prakash Nair, Sudha B. Kisan Yeri, Sharanabasappa B Vinayan, Madhumal Thayil Zaidi, Pervez H. |
| author_browse | B. Kisan Kuchanur, Prakash Nair, Sudha Patil, Ayyanagouda Somanatha Vinayan, Madhumal Thayil Yeri, Sharanabasappa B Zaidi, Pervez H. |
| author_facet | Somanatha Patil, Ayyanagouda Kuchanur, Prakash Nair, Sudha B. Kisan Yeri, Sharanabasappa B Vinayan, Madhumal Thayil Zaidi, Pervez H. |
| author_sort | Somanatha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sweet corn (Zea mays L. saccharata) is an important specialty maize, valued for its sweetness and nutritional quality with high consumer demand. The development of superior hybrids relies on genetically diverse inbred lines. Advances in genotyping technologies have transformed the way breeding programs manage their genetic resources. The identification of Single Nucleotide Polymorphisms (SNPs) can improve understanding of the molecular diversity of sweet corn inbred lines and their classification into heterotic groups, which is useful in determining certain crosses to obtain hybrids with higher yield performance. To evaluate molecular diversity, 23 sweet corn inbred lines were genotyped using 97 genome-wide single nucleotide polymorphism (SNP) markers through Kompetitive Allele-Specific PCR (KASP) during 2024-25 at the University of Agricultural Sciences, Raichur, Karnataka. After quality filtering, 56.04% of markers were polymorphic, with polymorphism information content (PIC) values ranging from 0 to 0.375 (mean = 0.39), indicating moderate genetic variability. Cluster analysis using the unweighted pair group method with arithmetic mean (UPGMA) grouped the lines into three major clusters, with most inbreds concentrated in Cluster I, while Clusters II and III contained divergent sweet corn inbred lines (SC-40, SC-9, SC-34 and SC-16), highlighting their potential as valuable sweet corn inbred lines. Principal component analysis (PCA) corroborated the clustering, identifying SC-34, SC-40 and SC-16 as distinct from the core group. The results demonstrate that SNP-based genotyping effectively reveals genetic structure and diversity in sweet corn, further providing a robust framework for heterotic grouping and informed parental selection in hybrid breeding programs. |
| format | Journal Article |
| id | CGSpace178397 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | ScienceDomain International |
| publisherStr | ScienceDomain International |
| record_format | dspace |
| spelling | CGSpace1783972025-12-01T02:07:28Z Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers Somanatha Patil, Ayyanagouda Kuchanur, Prakash Nair, Sudha B. Kisan Yeri, Sharanabasappa B Vinayan, Madhumal Thayil Zaidi, Pervez H. genetic diversity (resource) heterotic groups hybrids single nucleotide polymorphisms sweet corn Sweet corn (Zea mays L. saccharata) is an important specialty maize, valued for its sweetness and nutritional quality with high consumer demand. The development of superior hybrids relies on genetically diverse inbred lines. Advances in genotyping technologies have transformed the way breeding programs manage their genetic resources. The identification of Single Nucleotide Polymorphisms (SNPs) can improve understanding of the molecular diversity of sweet corn inbred lines and their classification into heterotic groups, which is useful in determining certain crosses to obtain hybrids with higher yield performance. To evaluate molecular diversity, 23 sweet corn inbred lines were genotyped using 97 genome-wide single nucleotide polymorphism (SNP) markers through Kompetitive Allele-Specific PCR (KASP) during 2024-25 at the University of Agricultural Sciences, Raichur, Karnataka. After quality filtering, 56.04% of markers were polymorphic, with polymorphism information content (PIC) values ranging from 0 to 0.375 (mean = 0.39), indicating moderate genetic variability. Cluster analysis using the unweighted pair group method with arithmetic mean (UPGMA) grouped the lines into three major clusters, with most inbreds concentrated in Cluster I, while Clusters II and III contained divergent sweet corn inbred lines (SC-40, SC-9, SC-34 and SC-16), highlighting their potential as valuable sweet corn inbred lines. Principal component analysis (PCA) corroborated the clustering, identifying SC-34, SC-40 and SC-16 as distinct from the core group. The results demonstrate that SNP-based genotyping effectively reveals genetic structure and diversity in sweet corn, further providing a robust framework for heterotic grouping and informed parental selection in hybrid breeding programs. 2025-10 2025-11-30T21:27:36Z 2025-11-30T21:27:36Z Journal Article https://hdl.handle.net/10568/178397 en Open Access application/pdf ScienceDomain International Somanatha, N., Patil, A., Kuchanur, P., Nair, S., B, K., Yeri, S. B., Vinayan, M., & Zaidi, P. (2025). Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers. Journal of Advances in Biology & Biotechnology, 28(10), 977-985. https://doi.org/10.9734/jabb/2025/v28i103119 |
| spellingShingle | genetic diversity (resource) heterotic groups hybrids single nucleotide polymorphisms sweet corn Somanatha Patil, Ayyanagouda Kuchanur, Prakash Nair, Sudha B. Kisan Yeri, Sharanabasappa B Vinayan, Madhumal Thayil Zaidi, Pervez H. Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers |
| title | Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers |
| title_full | Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers |
| title_fullStr | Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers |
| title_full_unstemmed | Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers |
| title_short | Molecular diversity studies in sweet corn inbred lines using Single Nucleotide Polymorphic markers |
| title_sort | molecular diversity studies in sweet corn inbred lines using single nucleotide polymorphic markers |
| topic | genetic diversity (resource) heterotic groups hybrids single nucleotide polymorphisms sweet corn |
| url | https://hdl.handle.net/10568/178397 |
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