Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers

Advances in genotyping technologies have changed how breeders study and manage maize genetics. Identification of single nucleotide polymorphisms (SNPs) helped researchers understand the molecular diversity among tropical maize (Zea mays L.) inbred lines, The present study was conducted to assess the...

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Autores principales: Sharanu S G, Patil, Ayyanagouda, Kuchanur, Prakash, Nair, Sudha, B. Kisan, Yeri, Sharanabasappa B, Vinayan, Madhumal Thayil, Zaidi, Pervez H.
Formato: Journal Article
Lenguaje:Inglés
Publicado: ScienceDomain International 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/178360
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author Sharanu S G
Patil, Ayyanagouda
Kuchanur, Prakash
Nair, Sudha
B. Kisan
Yeri, Sharanabasappa B
Vinayan, Madhumal Thayil
Zaidi, Pervez H.
author_browse B. Kisan
Kuchanur, Prakash
Nair, Sudha
Patil, Ayyanagouda
Sharanu S G
Vinayan, Madhumal Thayil
Yeri, Sharanabasappa B
Zaidi, Pervez H.
author_facet Sharanu S G
Patil, Ayyanagouda
Kuchanur, Prakash
Nair, Sudha
B. Kisan
Yeri, Sharanabasappa B
Vinayan, Madhumal Thayil
Zaidi, Pervez H.
author_sort Sharanu S G
collection Repository of Agricultural Research Outputs (CGSpace)
description Advances in genotyping technologies have changed how breeders study and manage maize genetics. Identification of single nucleotide polymorphisms (SNPs) helped researchers understand the molecular diversity among tropical maize (Zea mays L.) inbred lines, The present study was conducted to assess the molecular diversity and population structure of 107 tropical maize inbred lines collected from working germplasm programs using SNP markers. A total of 97 genome-wide SNP markers were employed for genotyping through the KASP assay. The polymorphism information content (PIC) values ranged from 0.018 to 0.375 with an overall mean of 0.285, indicating that the majority of markers were moderately informative. Hierarchical clustering grouped the inbred lines into three major clusters, reflecting clear genetic differentiation among the tropical maize inbred lines. Principal component analysis (PCA) further validated these findings by identifying highly divergent lines Viz., AHG-122, CIMMYT-22, BHG-20 and AHG-110-1. The congruence between UPGMA clustering and PCA confirmed the robustness of the diversity analysis. These findings provide valuable insights into the genetic relationships among tropical maize inbreds and highlight the utility of SNP markers in guiding the selection of parental lines for hybrid development.
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spelling CGSpace1783602025-11-28T02:15:00Z Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers Sharanu S G Patil, Ayyanagouda Kuchanur, Prakash Nair, Sudha B. Kisan Yeri, Sharanabasappa B Vinayan, Madhumal Thayil Zaidi, Pervez H. polymorphism single nucleotide polymorphisms genetic markers inbred lines Advances in genotyping technologies have changed how breeders study and manage maize genetics. Identification of single nucleotide polymorphisms (SNPs) helped researchers understand the molecular diversity among tropical maize (Zea mays L.) inbred lines, The present study was conducted to assess the molecular diversity and population structure of 107 tropical maize inbred lines collected from working germplasm programs using SNP markers. A total of 97 genome-wide SNP markers were employed for genotyping through the KASP assay. The polymorphism information content (PIC) values ranged from 0.018 to 0.375 with an overall mean of 0.285, indicating that the majority of markers were moderately informative. Hierarchical clustering grouped the inbred lines into three major clusters, reflecting clear genetic differentiation among the tropical maize inbred lines. Principal component analysis (PCA) further validated these findings by identifying highly divergent lines Viz., AHG-122, CIMMYT-22, BHG-20 and AHG-110-1. The congruence between UPGMA clustering and PCA confirmed the robustness of the diversity analysis. These findings provide valuable insights into the genetic relationships among tropical maize inbreds and highlight the utility of SNP markers in guiding the selection of parental lines for hybrid development. 2025-10 2025-11-27T22:41:54Z 2025-11-27T22:41:54Z Journal Article https://hdl.handle.net/10568/178360 en Open Access application/pdf ScienceDomain International Sharanu, S. G., Patil, A., Kuchanur, P. H., Nair, S., Kisan, B., Yeri, S. B., Vinayan, M. T., & Zaidi, P. H. (2025). Assessing molecular diversity of tropical maize inbred lines using single nucleotide polymorphic (SNP) markers. Journal of Experimental Agriculture International, 47(10), 490–500. https://doi.org/10.9734/jeai/2025/v47i103831
spellingShingle polymorphism
single nucleotide polymorphisms
genetic markers
inbred lines
Sharanu S G
Patil, Ayyanagouda
Kuchanur, Prakash
Nair, Sudha
B. Kisan
Yeri, Sharanabasappa B
Vinayan, Madhumal Thayil
Zaidi, Pervez H.
Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers
title Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers
title_full Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers
title_fullStr Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers
title_full_unstemmed Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers
title_short Assessing molecular diversity of tropical maize inbred lines using Single Nucleotide Polymorphic (SNP) markers
title_sort assessing molecular diversity of tropical maize inbred lines using single nucleotide polymorphic snp markers
topic polymorphism
single nucleotide polymorphisms
genetic markers
inbred lines
url https://hdl.handle.net/10568/178360
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