Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization
In wheat breeding programs, several hundred crosses are performed annually, but only individuals from a few families advance to the final stages of the breeding pipelines. Therefore, a deeper understanding of the general combining ability (GCA) of wheat genotypes might enhance the breeding efficienc...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2025
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| Acceso en línea: | https://hdl.handle.net/10568/178354 |
| _version_ | 1855514466794340352 |
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| author | Saavedra-Avila, José Ignacio Gerard, Guillermo Sebastián Esposito, Salvatore Velu, Govindan Huerta-Espino, Julio Tarekegn, Zerihun Tadesse Dreisigacker, Susanne Saint Pierre, Carolina Pacheco Gil, Rosa Angela Toledo, Fernando Henrique Gardner, Keith Crespo Herrera, Leonardo Abdiel Crossa, Jose Vitale, Paolo |
| author_browse | Crespo Herrera, Leonardo Abdiel Crossa, Jose Dreisigacker, Susanne Esposito, Salvatore Gardner, Keith Gerard, Guillermo Sebastián Huerta-Espino, Julio Pacheco Gil, Rosa Angela Saavedra-Avila, José Ignacio Saint Pierre, Carolina Tarekegn, Zerihun Tadesse Toledo, Fernando Henrique Velu, Govindan Vitale, Paolo |
| author_facet | Saavedra-Avila, José Ignacio Gerard, Guillermo Sebastián Esposito, Salvatore Velu, Govindan Huerta-Espino, Julio Tarekegn, Zerihun Tadesse Dreisigacker, Susanne Saint Pierre, Carolina Pacheco Gil, Rosa Angela Toledo, Fernando Henrique Gardner, Keith Crespo Herrera, Leonardo Abdiel Crossa, Jose Vitale, Paolo |
| author_sort | Saavedra-Avila, José Ignacio |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In wheat breeding programs, several hundred crosses are performed annually, but only individuals from a few families advance to the final stages of the breeding pipelines. Therefore, a deeper understanding of the general combining ability (GCA) of wheat genotypes might enhance the breeding efficiency in selecting parents. For this reason, we tested the performance of the offspring of similar to 1200 parental elite lines. Using a genome-wide association study (GWAS), gene ontology (GO) analysis, and genomic prediction (GP), our objectives were to i) identify marker-trait associates (MTAs) and candidate genes, ii) assess temporal allele frequency dynamics of identified MTAs, and iii) estimate prediction accuracy (PA) for key traits: Progeny Number per-Cross (PNC), grain yield (GY), and a combined index incorporating these traits ("index"). Our findings revealed a total of 13 MTAs: eight for GY, four for the "index", and one for PNC. The GO analysis highlighted several genes involved in hydrogen peroxide metabolism and catabolism processes (H2O2), reactive oxygen species, response to oxidative stress, cell wall biogenesis, the metabolic process of modified amino acids at the cellular level, and glutathione metabolic process for the studied traits. Notably, allele frequency analysis over time indicated that most MTAs are under positive selection, likely reflecting indirect breeder-driven selection. The highest PA was reached by using the reproducing kernel Hilbert space (RKHS) model for the trait GY (0.34). The identification of MTAs for PNC and GY provided insight into the biological pathways underpinning combining ability and demonstrated the potential for predicting the ability of the genotypes to be crossed. These findings might contribute to the optimization crossing strategy saving costs and increasing the breeding program efficiency. |
| format | Journal Article |
| id | CGSpace178354 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1783542025-11-28T02:01:01Z Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization Saavedra-Avila, José Ignacio Gerard, Guillermo Sebastián Esposito, Salvatore Velu, Govindan Huerta-Espino, Julio Tarekegn, Zerihun Tadesse Dreisigacker, Susanne Saint Pierre, Carolina Pacheco Gil, Rosa Angela Toledo, Fernando Henrique Gardner, Keith Crespo Herrera, Leonardo Abdiel Crossa, Jose Vitale, Paolo wheat breeding combining ability genome-wide association studies forecasting efficiency In wheat breeding programs, several hundred crosses are performed annually, but only individuals from a few families advance to the final stages of the breeding pipelines. Therefore, a deeper understanding of the general combining ability (GCA) of wheat genotypes might enhance the breeding efficiency in selecting parents. For this reason, we tested the performance of the offspring of similar to 1200 parental elite lines. Using a genome-wide association study (GWAS), gene ontology (GO) analysis, and genomic prediction (GP), our objectives were to i) identify marker-trait associates (MTAs) and candidate genes, ii) assess temporal allele frequency dynamics of identified MTAs, and iii) estimate prediction accuracy (PA) for key traits: Progeny Number per-Cross (PNC), grain yield (GY), and a combined index incorporating these traits ("index"). Our findings revealed a total of 13 MTAs: eight for GY, four for the "index", and one for PNC. The GO analysis highlighted several genes involved in hydrogen peroxide metabolism and catabolism processes (H2O2), reactive oxygen species, response to oxidative stress, cell wall biogenesis, the metabolic process of modified amino acids at the cellular level, and glutathione metabolic process for the studied traits. Notably, allele frequency analysis over time indicated that most MTAs are under positive selection, likely reflecting indirect breeder-driven selection. The highest PA was reached by using the reproducing kernel Hilbert space (RKHS) model for the trait GY (0.34). The identification of MTAs for PNC and GY provided insight into the biological pathways underpinning combining ability and demonstrated the potential for predicting the ability of the genotypes to be crossed. These findings might contribute to the optimization crossing strategy saving costs and increasing the breeding program efficiency. 2025-10-17 2025-11-27T20:21:42Z 2025-11-27T20:21:42Z Journal Article https://hdl.handle.net/10568/178354 en Open Access application/pdf Frontiers Media Saavedra-Ávila, J. I., Gerard, G. S., Esposito, S., Govindan, V., Huerta-Espino, J., Tadesse, Z., Dreisigacker, S., Saint Pierre, C., Pacheco, A., Toledo, F., Gardner, K. A., Crespo-Herrera, L., Crossa, J., & Vitale, P. (2025). Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization. Frontiers in Plant Science, 16, 1675993. https://doi.org/10.3389/fpls.2025.1675993 |
| spellingShingle | wheat breeding combining ability genome-wide association studies forecasting efficiency Saavedra-Avila, José Ignacio Gerard, Guillermo Sebastián Esposito, Salvatore Velu, Govindan Huerta-Espino, Julio Tarekegn, Zerihun Tadesse Dreisigacker, Susanne Saint Pierre, Carolina Pacheco Gil, Rosa Angela Toledo, Fernando Henrique Gardner, Keith Crespo Herrera, Leonardo Abdiel Crossa, Jose Vitale, Paolo Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization |
| title | Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization |
| title_full | Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization |
| title_fullStr | Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization |
| title_full_unstemmed | Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization |
| title_short | Unraveling the genetic basis of general combining ability in CIMMYT elite bread wheat germplasm: implications for breeding strategies optimization |
| title_sort | unraveling the genetic basis of general combining ability in cimmyt elite bread wheat germplasm implications for breeding strategies optimization |
| topic | wheat breeding combining ability genome-wide association studies forecasting efficiency |
| url | https://hdl.handle.net/10568/178354 |
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