Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel

Septoria nodorum blotch (SNB) and tan spot (TS) are two globally significant foliar diseases affecting wheat, causing substantial reductions in both yield and grain quality. In this study, genome-wide association study (GWAS) was conducted using an elite diversity panel comprising 150 lines to ident...

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Autores principales: Dehua Wang, Xinyao He, Zhiying Deng, Reynolds, Matthew P., Dreisigacker, Susanne, Pawan Kumar Singh
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/178130
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author Dehua Wang
Xinyao He
Zhiying Deng
Reynolds, Matthew P.
Dreisigacker, Susanne
Pawan Kumar Singh
author_browse Dehua Wang
Dreisigacker, Susanne
Pawan Kumar Singh
Reynolds, Matthew P.
Xinyao He
Zhiying Deng
author_facet Dehua Wang
Xinyao He
Zhiying Deng
Reynolds, Matthew P.
Dreisigacker, Susanne
Pawan Kumar Singh
author_sort Dehua Wang
collection Repository of Agricultural Research Outputs (CGSpace)
description Septoria nodorum blotch (SNB) and tan spot (TS) are two globally significant foliar diseases affecting wheat, causing substantial reductions in both yield and grain quality. In this study, genome-wide association study (GWAS) was conducted using an elite diversity panel comprising 150 lines to identify genetic loci associated with resistance to SNB and TS. Resistance was evaluated in greenhouse experiments at the seedling stage, with two replicates for each disease. For SNB, the majority of lines demonstrated good level of resistance, with 53% rated as resistant or moderately resistant (R/MR). Similarly, for TS, 60% of the lines exhibited R/MR resistance. Some lines exhibited high resistance to both SNB and TS. The panel was genotyped with the Illumina Infinium 25 K BeadChip. GWAS revealed several significant marker-trait associations on chromosome 5B associated with SNB resistance, all of which were located in the vicinity of the Tsn1 gene, suggesting its important role in conferring SNB susceptibility within this population. In addition, two quantitative trait loci (QTL) on chromosomes 2AL and 7AS were identified. For TS, significant markers were primarily found within a 20 Mb region on the long arm of chromosome 7B, with phenotypic variation explained ranging from 8.34% to 12.31%. Additional TS QTL with minor effects were identified on chromosomes 3A, 5A, 7A, and 7D. These resistant lines and identification of markers for SNB and TS resistance hold potential for use in wheat breeding programs aimed at improving resistance to the two diseases.
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spelling CGSpace1781302025-12-04T05:33:29Z Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel Dehua Wang Xinyao He Zhiying Deng Reynolds, Matthew P. Dreisigacker, Susanne Pawan Kumar Singh soft wheat disease resistance genome-wide association studies pyrenophora tritici-repentis leptosphaeria nodorum Septoria nodorum blotch (SNB) and tan spot (TS) are two globally significant foliar diseases affecting wheat, causing substantial reductions in both yield and grain quality. In this study, genome-wide association study (GWAS) was conducted using an elite diversity panel comprising 150 lines to identify genetic loci associated with resistance to SNB and TS. Resistance was evaluated in greenhouse experiments at the seedling stage, with two replicates for each disease. For SNB, the majority of lines demonstrated good level of resistance, with 53% rated as resistant or moderately resistant (R/MR). Similarly, for TS, 60% of the lines exhibited R/MR resistance. Some lines exhibited high resistance to both SNB and TS. The panel was genotyped with the Illumina Infinium 25 K BeadChip. GWAS revealed several significant marker-trait associations on chromosome 5B associated with SNB resistance, all of which were located in the vicinity of the Tsn1 gene, suggesting its important role in conferring SNB susceptibility within this population. In addition, two quantitative trait loci (QTL) on chromosomes 2AL and 7AS were identified. For TS, significant markers were primarily found within a 20 Mb region on the long arm of chromosome 7B, with phenotypic variation explained ranging from 8.34% to 12.31%. Additional TS QTL with minor effects were identified on chromosomes 3A, 5A, 7A, and 7D. These resistant lines and identification of markers for SNB and TS resistance hold potential for use in wheat breeding programs aimed at improving resistance to the two diseases. 2025-10 2025-11-24T16:58:17Z 2025-11-24T16:58:17Z Journal Article https://hdl.handle.net/10568/178130 en Limited Access Springer Wang, D., He, X., Deng, Z., Reynolds, M., Dreisigacker, S., & Singh, P. K. (2025). Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel. Molecular Breeding, 45(11), 85. https://doi.org/10.1007/s11032-025-01602-z
spellingShingle soft wheat
disease resistance
genome-wide association studies
pyrenophora tritici-repentis
leptosphaeria nodorum
Dehua Wang
Xinyao He
Zhiying Deng
Reynolds, Matthew P.
Dreisigacker, Susanne
Pawan Kumar Singh
Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel
title Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel
title_full Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel
title_fullStr Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel
title_full_unstemmed Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel
title_short Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel
title_sort genome wide association mapping for resistance against septoria nodorum blotch and tan spot in a diverse wheat panel
topic soft wheat
disease resistance
genome-wide association studies
pyrenophora tritici-repentis
leptosphaeria nodorum
url https://hdl.handle.net/10568/178130
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