Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections
Many forage species exhibit seed dormancy, which prevents germination and poses challenges for seed growers and analysts. Various methods have been developed to overcome dormancy in different forage species, but their effectiveness varies depending on the species and environmental conditions during...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
International Seed Testing Association
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/178102 |
| _version_ | 1855518966691135488 |
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| author | Olbana, Tamiru Hay, F.R. Negawo, Alemayehu T. Muchugi, A. Jones, Christopher S. |
| author_browse | Hay, F.R. Jones, Christopher S. Muchugi, A. Negawo, Alemayehu T. Olbana, Tamiru |
| author_facet | Olbana, Tamiru Hay, F.R. Negawo, Alemayehu T. Muchugi, A. Jones, Christopher S. |
| author_sort | Olbana, Tamiru |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Many forage species exhibit seed dormancy, which prevents germination and poses challenges for seed growers and analysts. Various methods have been developed to overcome dormancy in different forage species, but their effectiveness varies depending on the species and environmental conditions during seed development and storage. The seeds of <i>Chamaecytisus proliferus</i> exhibit dormancy due to a hard seed coat and lens covering the embryo. To overcome this dormancy, we evaluated physical, mechanical and chemical scarification methods. Among the treatments, excised embryos achieved the highest germination proportion (0.95). Hot water treatment for 15, 20 and 25 minutes effectively broke dormancy, resulting in proportions of 0.89, 0.92 and 0.89 germinated seeds, respectively. Treatment with 0.1 or 0.2% KNO<sub>3</sub> also enhanced germination, whereas a 0.3% solution led to lower germination (0.73). Exposure to concentrated H<sub>2</sub>SO<sub>4</sub> increased germination but caused a high proportion of dead seeds and abnormal seedlings. Similarly, mechanical scarification using a scalpel blade resulted in low germination (0.70) and a high proportion of seedling abnormalities (0.3). All pre-treatments reduced mean germination time, with excised embryos germinating fastest. In conclusion, excised embryos and hot water treatment (15‐25 minutes) effectively break dormancy and enhance germination in <i>Chamaecytisus proliferus</i>. |
| format | Journal Article |
| id | CGSpace178102 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | International Seed Testing Association |
| publisherStr | International Seed Testing Association |
| record_format | dspace |
| spelling | CGSpace1781022025-11-24T07:31:47Z Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections Olbana, Tamiru Hay, F.R. Negawo, Alemayehu T. Muchugi, A. Jones, Christopher S. forage gene banks seeds Many forage species exhibit seed dormancy, which prevents germination and poses challenges for seed growers and analysts. Various methods have been developed to overcome dormancy in different forage species, but their effectiveness varies depending on the species and environmental conditions during seed development and storage. The seeds of <i>Chamaecytisus proliferus</i> exhibit dormancy due to a hard seed coat and lens covering the embryo. To overcome this dormancy, we evaluated physical, mechanical and chemical scarification methods. Among the treatments, excised embryos achieved the highest germination proportion (0.95). Hot water treatment for 15, 20 and 25 minutes effectively broke dormancy, resulting in proportions of 0.89, 0.92 and 0.89 germinated seeds, respectively. Treatment with 0.1 or 0.2% KNO<sub>3</sub> also enhanced germination, whereas a 0.3% solution led to lower germination (0.73). Exposure to concentrated H<sub>2</sub>SO<sub>4</sub> increased germination but caused a high proportion of dead seeds and abnormal seedlings. Similarly, mechanical scarification using a scalpel blade resulted in low germination (0.70) and a high proportion of seedling abnormalities (0.3). All pre-treatments reduced mean germination time, with excised embryos germinating fastest. In conclusion, excised embryos and hot water treatment (15‐25 minutes) effectively break dormancy and enhance germination in <i>Chamaecytisus proliferus</i>. 2025-08-01 2025-11-24T07:11:08Z 2025-11-24T07:11:08Z Journal Article https://hdl.handle.net/10568/178102 en Open Access International Seed Testing Association Olbana, T., Hay, F.R., Negawo, A.T., Muchugi, A. and Jones, C.S. 2025. Optimisation of the germination test protocol for viability monitoring of <i>Chamaecytisus proliferus</i> seeds in genebank collections. Seed Science and Technology 53 (2): 211-223. |
| spellingShingle | forage gene banks seeds Olbana, Tamiru Hay, F.R. Negawo, Alemayehu T. Muchugi, A. Jones, Christopher S. Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections |
| title | Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections |
| title_full | Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections |
| title_fullStr | Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections |
| title_full_unstemmed | Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections |
| title_short | Optimisation of the germination test protocol for viability monitoring of Chamaecytisus proliferus seeds in genebank collections |
| title_sort | optimisation of the germination test protocol for viability monitoring of chamaecytisus proliferus seeds in genebank collections |
| topic | forage gene banks seeds |
| url | https://hdl.handle.net/10568/178102 |
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