Nematode resistance screening in Musa Spp. using macropropagated plantlets

This study evaluated macropropagated plantlets for nematode resistance screening in Musa spp. We aimed to determine the optimal timing for assessing resistance to Radopholus similis and Meloidogyne arenaria in the plantain cultivar ‘Agbagba’ at 30-, 60-, and 90-days post-inoculation (DPI). Additiona...

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Main Authors: Olajide, E., Cortada, L., Saikai, K., Amah, D., Coyne, D., Bert, W., Swennen, R., Kolombia, Y.A.
Format: Journal Article
Language:Inglés
Published: 2025
Subjects:
Online Access:https://hdl.handle.net/10568/177798
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author Olajide, E.
Cortada, L.
Saikai, K.
Amah, D.
Coyne, D.
Bert, W.
Swennen, R.
Kolombia, Y.A.
author_browse Amah, D.
Bert, W.
Cortada, L.
Coyne, D.
Kolombia, Y.A.
Olajide, E.
Saikai, K.
Swennen, R.
author_facet Olajide, E.
Cortada, L.
Saikai, K.
Amah, D.
Coyne, D.
Bert, W.
Swennen, R.
Kolombia, Y.A.
author_sort Olajide, E.
collection Repository of Agricultural Research Outputs (CGSpace)
description This study evaluated macropropagated plantlets for nematode resistance screening in Musa spp. We aimed to determine the optimal timing for assessing resistance to Radopholus similis and Meloidogyne arenaria in the plantain cultivar ‘Agbagba’ at 30-, 60-, and 90-days post-inoculation (DPI). Additionally, we tested different substrates for early-stage screening at 7 DPI. Results indicated that 60 DPI is optimal for resistance screening against R. similis due to significant root damage, while M. arenaria reproduction peaked at 90 DPI without extensive root deterioration. The sand-absorbent polymer (SAP) substrate was most effective for early nematode penetration. Using these findings, we assessed five Musa genotypes at 60 DPI; ‘SH 3142’ and ‘Pisang Jari Buaya’ showed resistance to R. similis, ‘Agbagba’ was susceptible, ‘Yangambi KM5’ was unexpectedly susceptible, and ‘Pisang Lilin’ showed intermediate resistance. This study highlights that macropropagated plantlets can be used as an effective method for rapid preliminary resistance screening in Musa spp.
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spelling CGSpace1777982025-12-08T10:06:44Z Nematode resistance screening in Musa Spp. using macropropagated plantlets Olajide, E. Cortada, L. Saikai, K. Amah, D. Coyne, D. Bert, W. Swennen, R. Kolombia, Y.A. plantains musa nematodes meloidogyne phenotypes radopholus similis susceptibility This study evaluated macropropagated plantlets for nematode resistance screening in Musa spp. We aimed to determine the optimal timing for assessing resistance to Radopholus similis and Meloidogyne arenaria in the plantain cultivar ‘Agbagba’ at 30-, 60-, and 90-days post-inoculation (DPI). Additionally, we tested different substrates for early-stage screening at 7 DPI. Results indicated that 60 DPI is optimal for resistance screening against R. similis due to significant root damage, while M. arenaria reproduction peaked at 90 DPI without extensive root deterioration. The sand-absorbent polymer (SAP) substrate was most effective for early nematode penetration. Using these findings, we assessed five Musa genotypes at 60 DPI; ‘SH 3142’ and ‘Pisang Jari Buaya’ showed resistance to R. similis, ‘Agbagba’ was susceptible, ‘Yangambi KM5’ was unexpectedly susceptible, and ‘Pisang Lilin’ showed intermediate resistance. This study highlights that macropropagated plantlets can be used as an effective method for rapid preliminary resistance screening in Musa spp. 2025 2025-11-11T13:05:04Z 2025-11-11T13:05:04Z Journal Article https://hdl.handle.net/10568/177798 en Limited Access Olajide, E., Cortada, L., Saikai, K., Amah, D., Coyne, D., Bert, W., ... & Kolombia, Y.A. (2025). Nematode resistance screening in Musa Spp. using macropropagated plantlets. Nematropica, 55(1), 18-31.
spellingShingle plantains
musa
nematodes
meloidogyne
phenotypes
radopholus similis
susceptibility
Olajide, E.
Cortada, L.
Saikai, K.
Amah, D.
Coyne, D.
Bert, W.
Swennen, R.
Kolombia, Y.A.
Nematode resistance screening in Musa Spp. using macropropagated plantlets
title Nematode resistance screening in Musa Spp. using macropropagated plantlets
title_full Nematode resistance screening in Musa Spp. using macropropagated plantlets
title_fullStr Nematode resistance screening in Musa Spp. using macropropagated plantlets
title_full_unstemmed Nematode resistance screening in Musa Spp. using macropropagated plantlets
title_short Nematode resistance screening in Musa Spp. using macropropagated plantlets
title_sort nematode resistance screening in musa spp using macropropagated plantlets
topic plantains
musa
nematodes
meloidogyne
phenotypes
radopholus similis
susceptibility
url https://hdl.handle.net/10568/177798
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