LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa
This research brief reports results from an inter-laboratory comparison (ILC) validating the Loop-Mediated Isothermal Amplification (LAMP) assay for virus detection in cassava and sweetpotato seed certification systems across East Africa. Conducted between October 2024 and May 2025, the study involv...
| Autor principal: | |
|---|---|
| Formato: | Brief |
| Lenguaje: | Inglés |
| Publicado: |
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/177177 |
| _version_ | 1855534835567689728 |
|---|---|
| author | Ogero, K. |
| author_browse | Ogero, K. |
| author_facet | Ogero, K. |
| author_sort | Ogero, K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This research brief reports results from an inter-laboratory comparison (ILC) validating the Loop-Mediated Isothermal Amplification (LAMP) assay for virus detection in cassava and sweetpotato seed certification systems across East Africa. Conducted between October 2024 and May 2025, the study involved four laboratories—KEPHIS (Kenya), TOSCI (Tanzania), IITA (Tanzania), and MAAIF (Uganda)—coordinated by the International Potato Center (CIP) under the PROSSIVA project. The LAMP method demonstrated high analytical sensitivity, detecting key viruses (SPFMV, SPCSV, SPLCV, CBSV, and UCBSV) within 30 minutes, with excellent reproducibility and no significant inter-laboratory variation. Results confirm LAMP’s suitability as a low-cost, rapid alternative to PCR for integration into national seed certification, quarantine, and phytosanitary systems, strengthening virus indexing capacity and harmonization across the region. |
| format | Brief |
| id | CGSpace177177 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| record_format | dspace |
| spelling | CGSpace1771772025-11-06T13:50:41Z LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa Ogero, K. cassava sweet potatoes seed certification diagnostic techniques This research brief reports results from an inter-laboratory comparison (ILC) validating the Loop-Mediated Isothermal Amplification (LAMP) assay for virus detection in cassava and sweetpotato seed certification systems across East Africa. Conducted between October 2024 and May 2025, the study involved four laboratories—KEPHIS (Kenya), TOSCI (Tanzania), IITA (Tanzania), and MAAIF (Uganda)—coordinated by the International Potato Center (CIP) under the PROSSIVA project. The LAMP method demonstrated high analytical sensitivity, detecting key viruses (SPFMV, SPCSV, SPLCV, CBSV, and UCBSV) within 30 minutes, with excellent reproducibility and no significant inter-laboratory variation. Results confirm LAMP’s suitability as a low-cost, rapid alternative to PCR for integration into national seed certification, quarantine, and phytosanitary systems, strengthening virus indexing capacity and harmonization across the region. 2025-10 2025-10-16T15:43:56Z 2025-10-16T15:43:56Z Brief https://hdl.handle.net/10568/177177 en Open Access application/pdf Ogero, K. 2025. LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa. International Potato Center. 2 p. |
| spellingShingle | cassava sweet potatoes seed certification diagnostic techniques Ogero, K. LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa |
| title | LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa |
| title_full | LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa |
| title_fullStr | LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa |
| title_full_unstemmed | LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa |
| title_short | LAMP Proves Reliable for Cassava and Sweetpotato Seed Certification as a Virus Detection Method in East Africa |
| title_sort | lamp proves reliable for cassava and sweetpotato seed certification as a virus detection method in east africa |
| topic | cassava sweet potatoes seed certification diagnostic techniques |
| url | https://hdl.handle.net/10568/177177 |
| work_keys_str_mv | AT ogerok lampprovesreliableforcassavaandsweetpotatoseedcertificationasavirusdetectionmethodineastafrica |