Elimination of sweet potato virus disease agents by meristem tip culture
Sweet potato vein clearing disease agents have been eliminated from 20 elite clones of sweet potato through meristem tip culture. Meristem tips of 0.25 to 0.4 mm length were cultured in a modified Murashige and Skoog medium. Plantlets were obtained after two months and transferred to soil. Indexing...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1981
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/177173 |
| _version_ | 1855522935507255296 |
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| author | Frison, E. Ng, S.Y. |
| author_browse | Frison, E. Ng, S.Y. |
| author_facet | Frison, E. Ng, S.Y. |
| author_sort | Frison, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sweet potato vein clearing disease agents have been eliminated from 20 elite clones of sweet potato through meristem tip culture. Meristem tips of 0.25 to 0.4 mm length were cultured in a modified Murashige and Skoog medium. Plantlets were obtained after two months and transferred to soil. Indexing was accomplished by approach grafting to Ipomoea setosa. About 80% of the plants obtained by meristem tip culture indexed negatively. Rapid multiplication was accomplished using single node cutting in vitro. A multiplication rate of 4.2 /month was obtained. The material can be stored in tissue culture for one year without transfer to fresh medium. Virus-free material of IITA elite clones of sweet potato is available for distribution. |
| format | Journal Article |
| id | CGSpace177173 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1981 |
| publishDateRange | 1981 |
| publishDateSort | 1981 |
| record_format | dspace |
| spelling | CGSpace1771732025-11-11T10:13:52Z Elimination of sweet potato virus disease agents by meristem tip culture Frison, E. Ng, S.Y. plant pathology sweet potatoes techniques Sweet potato vein clearing disease agents have been eliminated from 20 elite clones of sweet potato through meristem tip culture. Meristem tips of 0.25 to 0.4 mm length were cultured in a modified Murashige and Skoog medium. Plantlets were obtained after two months and transferred to soil. Indexing was accomplished by approach grafting to Ipomoea setosa. About 80% of the plants obtained by meristem tip culture indexed negatively. Rapid multiplication was accomplished using single node cutting in vitro. A multiplication rate of 4.2 /month was obtained. The material can be stored in tissue culture for one year without transfer to fresh medium. Virus-free material of IITA elite clones of sweet potato is available for distribution. 1981 2025-10-16T13:25:58Z 2025-10-16T13:25:58Z Journal Article https://hdl.handle.net/10568/177173 en Limited Access application/pdf Frison, E.A. & Ng, S.Y. (1981). Elimination of sweet potato virus disease agents by meristem tip culture. International Journal of Pest Management, 27(4), 452-454. |
| spellingShingle | plant pathology sweet potatoes techniques Frison, E. Ng, S.Y. Elimination of sweet potato virus disease agents by meristem tip culture |
| title | Elimination of sweet potato virus disease agents by meristem tip culture |
| title_full | Elimination of sweet potato virus disease agents by meristem tip culture |
| title_fullStr | Elimination of sweet potato virus disease agents by meristem tip culture |
| title_full_unstemmed | Elimination of sweet potato virus disease agents by meristem tip culture |
| title_short | Elimination of sweet potato virus disease agents by meristem tip culture |
| title_sort | elimination of sweet potato virus disease agents by meristem tip culture |
| topic | plant pathology sweet potatoes techniques |
| url | https://hdl.handle.net/10568/177173 |
| work_keys_str_mv | AT frisone eliminationofsweetpotatovirusdiseaseagentsbymeristemtipculture AT ngsy eliminationofsweetpotatovirusdiseaseagentsbymeristemtipculture |