Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces
Background: Drought is a major constraint to sorghum production. Developing drought-tolerant sorghum varieties is crucial. Optimal root system architecture (RSA) plays a vital role in plant adaptation and productivity under water- limited environment. Genetic assessments of untapped Ethiopian core s...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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BioMed Central
2025
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| Online Access: | https://hdl.handle.net/10568/176938 |
| _version_ | 1855543485514383360 |
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| author | Mitiku, Abel Debebe Feyissa, Tilaye Woldetensaye, Alemu Tirfessa Chikssa, Habte Nida Menamo, Temesgen Matiwos Abebe, Tewodros Mesfin Bante, Kassahun |
| author_browse | Abebe, Tewodros Mesfin Bante, Kassahun Chikssa, Habte Nida Feyissa, Tilaye Menamo, Temesgen Matiwos Mitiku, Abel Debebe Woldetensaye, Alemu Tirfessa |
| author_facet | Mitiku, Abel Debebe Feyissa, Tilaye Woldetensaye, Alemu Tirfessa Chikssa, Habte Nida Menamo, Temesgen Matiwos Abebe, Tewodros Mesfin Bante, Kassahun |
| author_sort | Mitiku, Abel Debebe |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Drought is a major constraint to sorghum production. Developing drought-tolerant sorghum varieties is crucial. Optimal root system architecture (RSA) plays a vital role in plant adaptation and productivity under water- limited environment. Genetic assessments of untapped Ethiopian core sorghum germplasm to identify quantitative trait nucleotides (QTN) for RSA traits are crucial. Therefore, this study aimed to identify genetic markers associated with seven RSA and related traits in 182 Ethiopian core sorghum-landraces using multi locus genome-wide association studies (ML-GWAS) using SNP markers.
Results: The germplasm exhibited substantial phenotypic variation for all measured traits. Genome-wide association analysis identified 181 significant QTNs (LOD ≥ 4) associated with nodal root angle (NRA), shoot traits (length, number, dry weight), and RSA (angle, number, dry weight) across six multi-locus GWAS models. Notably, 73 of these QTNs were consistently detected by at least two ML-GWAS methods. A total of 132 potential candidate genes, ranging from 13 to 25 genes per marker trait for the QTNs, were found about 18kbp LD decay of the significant QTNs using biomaRt. Of these, 13 genes were functionally annotated and involved in various biological processes.
Conclusions: Following the validation of candidate genes for root architecture and related traits, the core collection can be utilized to develop water-efficient sorghum varieties. |
| format | Journal Article |
| id | CGSpace176938 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | CGSpace1769382026-01-23T13:43:47Z Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces Mitiku, Abel Debebe Feyissa, Tilaye Woldetensaye, Alemu Tirfessa Chikssa, Habte Nida Menamo, Temesgen Matiwos Abebe, Tewodros Mesfin Bante, Kassahun genomes root architecture sorghum Background: Drought is a major constraint to sorghum production. Developing drought-tolerant sorghum varieties is crucial. Optimal root system architecture (RSA) plays a vital role in plant adaptation and productivity under water- limited environment. Genetic assessments of untapped Ethiopian core sorghum germplasm to identify quantitative trait nucleotides (QTN) for RSA traits are crucial. Therefore, this study aimed to identify genetic markers associated with seven RSA and related traits in 182 Ethiopian core sorghum-landraces using multi locus genome-wide association studies (ML-GWAS) using SNP markers. Results: The germplasm exhibited substantial phenotypic variation for all measured traits. Genome-wide association analysis identified 181 significant QTNs (LOD ≥ 4) associated with nodal root angle (NRA), shoot traits (length, number, dry weight), and RSA (angle, number, dry weight) across six multi-locus GWAS models. Notably, 73 of these QTNs were consistently detected by at least two ML-GWAS methods. A total of 132 potential candidate genes, ranging from 13 to 25 genes per marker trait for the QTNs, were found about 18kbp LD decay of the significant QTNs using biomaRt. Of these, 13 genes were functionally annotated and involved in various biological processes. Conclusions: Following the validation of candidate genes for root architecture and related traits, the core collection can be utilized to develop water-efficient sorghum varieties. 2025-09-02 2025-10-09T13:27:18Z 2025-10-09T13:27:18Z Journal Article https://hdl.handle.net/10568/176938 en Open Access application/pdf BioMed Central Mitiku, A.D.; Feyissa, T.; Woldetensaye, A.T.; Chikssa, H.N.; Menamo, T.M.; Abebe, T.M.; Bante, K. (2025) Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces. BMC Plant Biology 25: 1180. ISSN: 1471-2229 |
| spellingShingle | genomes root architecture sorghum Mitiku, Abel Debebe Feyissa, Tilaye Woldetensaye, Alemu Tirfessa Chikssa, Habte Nida Menamo, Temesgen Matiwos Abebe, Tewodros Mesfin Bante, Kassahun Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces |
| title | Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces |
| title_full | Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces |
| title_fullStr | Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces |
| title_full_unstemmed | Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces |
| title_short | Multi-locus genome-wide association studies for root system architectural traits in Ethiopian sorghum (Sorghum bicolor L.) landraces |
| title_sort | multi locus genome wide association studies for root system architectural traits in ethiopian sorghum sorghum bicolor l landraces |
| topic | genomes root architecture sorghum |
| url | https://hdl.handle.net/10568/176938 |
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