Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp
Cassava (Manihot esculenta Crantz) is a key crop for starch and biofuels production. This study focuses on the polysaccharide composition and saccharification efficiency in cassava pulp through genome-wide association studies (GWAS), targeting the improvement of root characteristics for industrial u...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/176803 |
| _version_ | 1855529768628256768 |
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| author | Sunvittayakul, Pongsakorn Wonnapinij, Passorn Wannitikul, Pitchaporn Phanthanong, Phongnapha Changwitchukarn, Kanokpoo Suttangkakul, Anongpat Utthiya, Supanut Phraemuang, Apimon Kongsil, Pasajee Prommarit, Kamonchat Ceballos, Hernan Gomez, Leonardo D. Kittipadakul, Piya Vuttipongchaikij, Supachai |
| author_browse | Ceballos, Hernan Changwitchukarn, Kanokpoo Gomez, Leonardo D. Kittipadakul, Piya Kongsil, Pasajee Phanthanong, Phongnapha Phraemuang, Apimon Prommarit, Kamonchat Sunvittayakul, Pongsakorn Suttangkakul, Anongpat Utthiya, Supanut Vuttipongchaikij, Supachai Wannitikul, Pitchaporn Wonnapinij, Passorn |
| author_facet | Sunvittayakul, Pongsakorn Wonnapinij, Passorn Wannitikul, Pitchaporn Phanthanong, Phongnapha Changwitchukarn, Kanokpoo Suttangkakul, Anongpat Utthiya, Supanut Phraemuang, Apimon Kongsil, Pasajee Prommarit, Kamonchat Ceballos, Hernan Gomez, Leonardo D. Kittipadakul, Piya Vuttipongchaikij, Supachai |
| author_sort | Sunvittayakul, Pongsakorn |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) is a key crop for starch and biofuels production. This study focuses on the polysaccharide composition and saccharification efficiency in cassava pulp through genome-wide association studies (GWAS), targeting the improvement of root characteristics for industrial use. We analyzed 135 partially inbred lines population, performing monosaccharide composition and saccharification analyses to reveal substantial variability in storage root biomass. Among 33 traits examined, 128 significant SNPs were associated with 23 biomass traits, highlighting a complex genetic architecture. Saccharification potential varied from 39 to 95 nmol Glu mg−1 h−1, with high broad-sense heritability for saccharification and several monosaccharide traits, indicating a strong genetic control. Our findings revealed that cassava pulp comprises similar proportions of pectin, hemicellulose, and cellulose in all genotypes. Correlation analysis showed significant associations between cellulose content and saccharification, suggesting that enhancing these traits can improve bioconversion efficiency. Negative correlations with glucose and glucuronic acid in hemicellulose and pectin fractions imply these components may inhibit saccharification. We identified 118 candidate genes associated with 21 traits, with many involved in stress responses affecting cell wall composition. This study verified 12 key candidate genes through sequence and expression analysis, including MANES_07G081200, a YTH domain-containing protein associated with saccharification. Several stress-response genes, such as MANES_04G118600 and MANES_09G174600, were linked to monosaccharide traits, suggesting that adaptive stress pathways influence biomass characteristics. This study provides insights into the genetic determinants of cassava pulp's saccharification and polysaccharide composition, aiding breeding efforts to develop cassava varieties optimized for industrial applications. |
| format | Journal Article |
| id | CGSpace176803 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1768032025-11-11T18:53:41Z Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp Sunvittayakul, Pongsakorn Wonnapinij, Passorn Wannitikul, Pitchaporn Phanthanong, Phongnapha Changwitchukarn, Kanokpoo Suttangkakul, Anongpat Utthiya, Supanut Phraemuang, Apimon Kongsil, Pasajee Prommarit, Kamonchat Ceballos, Hernan Gomez, Leonardo D. Kittipadakul, Piya Vuttipongchaikij, Supachai cassava composition cell walls genome-wide association studies hydrolysis pulp Cassava (Manihot esculenta Crantz) is a key crop for starch and biofuels production. This study focuses on the polysaccharide composition and saccharification efficiency in cassava pulp through genome-wide association studies (GWAS), targeting the improvement of root characteristics for industrial use. We analyzed 135 partially inbred lines population, performing monosaccharide composition and saccharification analyses to reveal substantial variability in storage root biomass. Among 33 traits examined, 128 significant SNPs were associated with 23 biomass traits, highlighting a complex genetic architecture. Saccharification potential varied from 39 to 95 nmol Glu mg−1 h−1, with high broad-sense heritability for saccharification and several monosaccharide traits, indicating a strong genetic control. Our findings revealed that cassava pulp comprises similar proportions of pectin, hemicellulose, and cellulose in all genotypes. Correlation analysis showed significant associations between cellulose content and saccharification, suggesting that enhancing these traits can improve bioconversion efficiency. Negative correlations with glucose and glucuronic acid in hemicellulose and pectin fractions imply these components may inhibit saccharification. We identified 118 candidate genes associated with 21 traits, with many involved in stress responses affecting cell wall composition. This study verified 12 key candidate genes through sequence and expression analysis, including MANES_07G081200, a YTH domain-containing protein associated with saccharification. Several stress-response genes, such as MANES_04G118600 and MANES_09G174600, were linked to monosaccharide traits, suggesting that adaptive stress pathways influence biomass characteristics. This study provides insights into the genetic determinants of cassava pulp's saccharification and polysaccharide composition, aiding breeding efforts to develop cassava varieties optimized for industrial applications. 2025-01 2025-10-03T08:25:19Z 2025-10-03T08:25:19Z Journal Article https://hdl.handle.net/10568/176803 en Open Access application/pdf Elsevier Sunvittayakul, P.; Wonnapinij, P.; Wannitikul, P.; Phanthanong, P.; Changwitchukarn, K.; Suttangkakul, A.; Utthiya, S.; Phraemuang, A.; Kongsil, P.; Prommarit, K.; Ceballos, H.; Gomez, L.D.; Kittipadakul, P.; Vuttipongchaikij, S. (2025) Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp. Plant Physiology and Biochemistry 218: 109312. ISSN: 0981-9428 |
| spellingShingle | cassava composition cell walls genome-wide association studies hydrolysis pulp Sunvittayakul, Pongsakorn Wonnapinij, Passorn Wannitikul, Pitchaporn Phanthanong, Phongnapha Changwitchukarn, Kanokpoo Suttangkakul, Anongpat Utthiya, Supanut Phraemuang, Apimon Kongsil, Pasajee Prommarit, Kamonchat Ceballos, Hernan Gomez, Leonardo D. Kittipadakul, Piya Vuttipongchaikij, Supachai Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| title | Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| title_full | Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| title_fullStr | Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| title_full_unstemmed | Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| title_short | Genome-wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| title_sort | genome wide association studies unveils the genetic basis of cell wall composition and saccharification of cassava pulp |
| topic | cassava composition cell walls genome-wide association studies hydrolysis pulp |
| url | https://hdl.handle.net/10568/176803 |
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