Optimizing a protocol for salinity recovery during seedling stage in rice
Salinity is one of the major abiotic stresses affecting rice production, but the levels of salinity in a given field are not constant across the growing season. Since the level of salinity in a rice field can fluctuate, fast recovery from salinity stress may be a useful trait to improve rice product...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Informa UK Limited
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/176711 |
| _version_ | 1855513136349577216 |
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| author | Siddique, Md. Abubakar Egdane, James Bagunu, Efren Quick, William Paul Diaz, Maria Genaleen Q. Henry, Amelia |
| author_browse | Bagunu, Efren Diaz, Maria Genaleen Q. Egdane, James Henry, Amelia Quick, William Paul Siddique, Md. Abubakar |
| author_facet | Siddique, Md. Abubakar Egdane, James Bagunu, Efren Quick, William Paul Diaz, Maria Genaleen Q. Henry, Amelia |
| author_sort | Siddique, Md. Abubakar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Salinity is one of the major abiotic stresses affecting rice production, but the levels of salinity in a given field are not constant across the growing season. Since the level of salinity in a rice field can fluctuate, fast recovery from salinity stress may be a useful trait to improve rice productivity in salinity-prone areas. To develop a protocol to screen for salinity recovery, seedling stage hydroponic experiments were conducted to measure salinity recovery over time through both destructive and high-throughput image-based phenotyping. Seven rice varieties were included that had previously been classified as tolerant or susceptible to salinity. Following exposure to seedling stage salinity, plants were transferred to solution with no added salt and allowed to recover. Green leaf area and relative growth rates (RGR) of salinity tolerant varieties and one salinity sensitive variety initiated recovery (i.e. started to increase) after 4 days of salt stress removal and required 6 days to completely recover (i.e. to resume a similar RGR to that observed in the no-salt control treatment), while the other salinity sensitive varieties took more time to recover. An optimal recovery period of 6 days after salt stress removal was identified for screening. Based on RGR and chlorophyll fluorescence values, some salinity sensitive varieties recovered while their Na+ contents remained high. Therefore, salinity tolerance may not necessarily correspond to salinity recovery ability. The protocol optimized here can be scaled up to screen diversity panels and populations and used for genetic mapping of the seedling stage salinity recovery trait. |
| format | Journal Article |
| id | CGSpace176711 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace1767112025-11-12T04:54:55Z Optimizing a protocol for salinity recovery during seedling stage in rice Siddique, Md. Abubakar Egdane, James Bagunu, Efren Quick, William Paul Diaz, Maria Genaleen Q. Henry, Amelia abiotic stress salt tolerance rice seedlings phenotypes chromosome mapping salinity recovery Salinity is one of the major abiotic stresses affecting rice production, but the levels of salinity in a given field are not constant across the growing season. Since the level of salinity in a rice field can fluctuate, fast recovery from salinity stress may be a useful trait to improve rice productivity in salinity-prone areas. To develop a protocol to screen for salinity recovery, seedling stage hydroponic experiments were conducted to measure salinity recovery over time through both destructive and high-throughput image-based phenotyping. Seven rice varieties were included that had previously been classified as tolerant or susceptible to salinity. Following exposure to seedling stage salinity, plants were transferred to solution with no added salt and allowed to recover. Green leaf area and relative growth rates (RGR) of salinity tolerant varieties and one salinity sensitive variety initiated recovery (i.e. started to increase) after 4 days of salt stress removal and required 6 days to completely recover (i.e. to resume a similar RGR to that observed in the no-salt control treatment), while the other salinity sensitive varieties took more time to recover. An optimal recovery period of 6 days after salt stress removal was identified for screening. Based on RGR and chlorophyll fluorescence values, some salinity sensitive varieties recovered while their Na+ contents remained high. Therefore, salinity tolerance may not necessarily correspond to salinity recovery ability. The protocol optimized here can be scaled up to screen diversity panels and populations and used for genetic mapping of the seedling stage salinity recovery trait. 2025-10-02 2025-09-30T08:32:38Z 2025-09-30T08:32:38Z Journal Article https://hdl.handle.net/10568/176711 en Open Access application/pdf Informa UK Limited Siddique, Md Abubakar, James Egdane, Efren Bagunu, William Paul Quick, Maria Genaleen Q. Diaz, and Amelia Henry. "Optimizing a protocol for salinity recovery during seedling stage in rice." Plant Production Science (2025): 1-15. |
| spellingShingle | abiotic stress salt tolerance rice seedlings phenotypes chromosome mapping salinity recovery Siddique, Md. Abubakar Egdane, James Bagunu, Efren Quick, William Paul Diaz, Maria Genaleen Q. Henry, Amelia Optimizing a protocol for salinity recovery during seedling stage in rice |
| title | Optimizing a protocol for salinity recovery during seedling stage in rice |
| title_full | Optimizing a protocol for salinity recovery during seedling stage in rice |
| title_fullStr | Optimizing a protocol for salinity recovery during seedling stage in rice |
| title_full_unstemmed | Optimizing a protocol for salinity recovery during seedling stage in rice |
| title_short | Optimizing a protocol for salinity recovery during seedling stage in rice |
| title_sort | optimizing a protocol for salinity recovery during seedling stage in rice |
| topic | abiotic stress salt tolerance rice seedlings phenotypes chromosome mapping salinity recovery |
| url | https://hdl.handle.net/10568/176711 |
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