Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa

In sub-Saharan Africa (SSA), plantains (Musa spp.) are a staple crop and a vital source of income for smallholder farmers. Despite their importance, the microbial diversity of soils in plantain-growing agroecologies remains poorly understood-particularly how it may influence plant performance and re...

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Main Authors: Kaushal, Manoj, Kolombia, Yao Adjiguita, Alakonya, Amos Emitati, Masso, Cargele
Format: Journal Article
Language:Inglés
Published: Nature Portfolio 2025
Subjects:
Online Access:https://hdl.handle.net/10568/176552
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author Kaushal, Manoj
Kolombia, Yao Adjiguita
Alakonya, Amos Emitati
Masso, Cargele
author_browse Alakonya, Amos Emitati
Kaushal, Manoj
Kolombia, Yao Adjiguita
Masso, Cargele
author_facet Kaushal, Manoj
Kolombia, Yao Adjiguita
Alakonya, Amos Emitati
Masso, Cargele
author_sort Kaushal, Manoj
collection Repository of Agricultural Research Outputs (CGSpace)
description In sub-Saharan Africa (SSA), plantains (Musa spp.) are a staple crop and a vital source of income for smallholder farmers. Despite their importance, the microbial diversity of soils in plantain-growing agroecologies remains poorly understood-particularly how it may influence plant performance and resilience through soil-plant interactions. In this study, we used Illumina MiSeq-based 16S rDNA sequencing to characterize bacterial communities in the rhizosphere of plantains cultivated under two distinct agroecological settings: Backyard gardens and main farms. Analyses of alpha and beta diversity (Sobs, Chao1, ACE, Shannon-Wiener, and Simpson indices; P < 0.05) revealed significant differences in species richness and community structure between the two agroecologies. Actinobacteria (55%) emerged as the dominant phylum, followed by Proteobacteria (21%) and Acidobacteria (15%). Beneficial genera such as Bacillus, Streptomyces, Bradyrhizobium, and Paenibacillus were also detected. Functional predictions based on COG and KEGG databases indicated notable differences in microbial functional potential between the two settings. These results suggest that agroecological context and habitat type strongly influence rhizosphere microbial diversity, with important implications for enhancing plant-microbe interactions and supporting crop resilience in SSA's resource-limited smallholder systems.
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spelling CGSpace1765522025-12-08T10:11:39Z Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa Kaushal, Manoj Kolombia, Yao Adjiguita Alakonya, Amos Emitati Masso, Cargele evaluation sustainable agriculture soil quality-soil health organic amendments microbial diversity metagenomics In sub-Saharan Africa (SSA), plantains (Musa spp.) are a staple crop and a vital source of income for smallholder farmers. Despite their importance, the microbial diversity of soils in plantain-growing agroecologies remains poorly understood-particularly how it may influence plant performance and resilience through soil-plant interactions. In this study, we used Illumina MiSeq-based 16S rDNA sequencing to characterize bacterial communities in the rhizosphere of plantains cultivated under two distinct agroecological settings: Backyard gardens and main farms. Analyses of alpha and beta diversity (Sobs, Chao1, ACE, Shannon-Wiener, and Simpson indices; P < 0.05) revealed significant differences in species richness and community structure between the two agroecologies. Actinobacteria (55%) emerged as the dominant phylum, followed by Proteobacteria (21%) and Acidobacteria (15%). Beneficial genera such as Bacillus, Streptomyces, Bradyrhizobium, and Paenibacillus were also detected. Functional predictions based on COG and KEGG databases indicated notable differences in microbial functional potential between the two settings. These results suggest that agroecological context and habitat type strongly influence rhizosphere microbial diversity, with important implications for enhancing plant-microbe interactions and supporting crop resilience in SSA's resource-limited smallholder systems. 2025-08-18 2025-09-18T06:00:25Z 2025-09-18T06:00:25Z Journal Article https://hdl.handle.net/10568/176552 en Open Access application/pdf Nature Portfolio Kaushal, M.; Kolombia, Y.A.; Alakonya, A.E.; Masso, C. (2025) Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa. Scientific Reports 15(1): 30220. ISSN: 2045-2322
spellingShingle evaluation
sustainable agriculture
soil quality-soil health
organic amendments
microbial diversity
metagenomics
Kaushal, Manoj
Kolombia, Yao Adjiguita
Alakonya, Amos Emitati
Masso, Cargele
Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa
title Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa
title_full Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa
title_fullStr Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa
title_full_unstemmed Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa
title_short Comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in Western and Central Africa
title_sort comparing soil microbial diversity in smallholder plantain backyard gardens and main farms in western and central africa
topic evaluation
sustainable agriculture
soil quality-soil health
organic amendments
microbial diversity
metagenomics
url https://hdl.handle.net/10568/176552
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