Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor
High temperature tends to be a contributing factor to the spread of vector-borne viral diseases by enhancing vector competence. However, the underlying mechanisms of the increased virus transmission capacity of vectors under heat stress are still largely unknown. Tomato yellow leaf curl virus (TYL...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/176088 |
| _version_ | 1855526248326889472 |
|---|---|
| author | Wang, Yu-Meng Xie, Ting He, Ya-Zhou Cuellar, Wilmer Jose Wang, Xiao-Wei |
| author_browse | Cuellar, Wilmer Jose He, Ya-Zhou Wang, Xiao-Wei Wang, Yu-Meng Xie, Ting |
| author_facet | Wang, Yu-Meng Xie, Ting He, Ya-Zhou Cuellar, Wilmer Jose Wang, Xiao-Wei |
| author_sort | Wang, Yu-Meng |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | High temperature tends to be a contributing factor to the spread of vector-borne viral diseases by enhancing vector
competence. However, the underlying mechanisms of the increased virus transmission capacity of vectors under heat
stress are still largely unknown. Tomato yellow leaf curl virus (TYLCV) is one of the most devastating plant DNA
viruses worldwide and is transmitted exclusively by Bemisia tabaci. Here, we identified heat shock factor (HSF) as a key
transcription factor that mediates TYLCV accumulation in whitefly vectors under heat stress. Quantitative analysis
revealed that the amount of TYLCV DNA in whiteflies gradually increased with increasing temperature (above 38 °C).
To determine the underlying mechanism, RNA-seq analysis was performed, which revealed that 1 h of heat stress
caused a dramatic increase in heat shock protein (HSP) expression. Moreover, the whitefly HSP transcription factor
HSF was shown to specifically interact with the intergenic region of TYLCV via yeast one-hybrid and dual-luciferase
analyses. Additionally, the transcriptional activity of HSF gradually increased with increasing heat treatment tempera‑
ture, indicating a similar pattern of virus accumulation under heat stress. Knocking down HSF in whiteflies caused
a significant decrease in TYLCV DNA and coat protein. Our results reveal the important role of HSF in mediating virus
accumulation in insect vectors under high temperature and provide insights into how environmental factors affect
plant virus‒vector interactions. |
| format | Journal Article |
| id | CGSpace176088 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1760882025-11-11T18:49:45Z Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor Wang, Yu-Meng Xie, Ting He, Ya-Zhou Cuellar, Wilmer Jose Wang, Xiao-Wei High temperature vector-borne diseases bemisia tabaci tomato yellow leaf curl virus gene transcription virus replication heat shock High temperature tends to be a contributing factor to the spread of vector-borne viral diseases by enhancing vector competence. However, the underlying mechanisms of the increased virus transmission capacity of vectors under heat stress are still largely unknown. Tomato yellow leaf curl virus (TYLCV) is one of the most devastating plant DNA viruses worldwide and is transmitted exclusively by Bemisia tabaci. Here, we identified heat shock factor (HSF) as a key transcription factor that mediates TYLCV accumulation in whitefly vectors under heat stress. Quantitative analysis revealed that the amount of TYLCV DNA in whiteflies gradually increased with increasing temperature (above 38 °C). To determine the underlying mechanism, RNA-seq analysis was performed, which revealed that 1 h of heat stress caused a dramatic increase in heat shock protein (HSP) expression. Moreover, the whitefly HSP transcription factor HSF was shown to specifically interact with the intergenic region of TYLCV via yeast one-hybrid and dual-luciferase analyses. Additionally, the transcriptional activity of HSF gradually increased with increasing heat treatment tempera‑ ture, indicating a similar pattern of virus accumulation under heat stress. Knocking down HSF in whiteflies caused a significant decrease in TYLCV DNA and coat protein. Our results reveal the important role of HSF in mediating virus accumulation in insect vectors under high temperature and provide insights into how environmental factors affect plant virus‒vector interactions. 2024-12-17 2025-08-13T08:10:56Z 2025-08-13T08:10:56Z Journal Article https://hdl.handle.net/10568/176088 en Open Access application/pdf Springer Wang, Y.; Xie, T.; He, Y.; Cuellar, W.J.; Wang, X. (2024) Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor. Crop Health 2(1): 19. ISSN: 2948-1945 |
| spellingShingle | High temperature vector-borne diseases bemisia tabaci tomato yellow leaf curl virus gene transcription virus replication heat shock Wang, Yu-Meng Xie, Ting He, Ya-Zhou Cuellar, Wilmer Jose Wang, Xiao-Wei Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| title | Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| title_full | Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| title_fullStr | Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| title_full_unstemmed | Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| title_short | Heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| title_sort | heat stress promotes the accumulation of tomato yellow leaf curl virus in its insect vector by activating heat shock factor |
| topic | High temperature vector-borne diseases bemisia tabaci tomato yellow leaf curl virus gene transcription virus replication heat shock |
| url | https://hdl.handle.net/10568/176088 |
| work_keys_str_mv | AT wangyumeng heatstresspromotestheaccumulationoftomatoyellowleafcurlvirusinitsinsectvectorbyactivatingheatshockfactor AT xieting heatstresspromotestheaccumulationoftomatoyellowleafcurlvirusinitsinsectvectorbyactivatingheatshockfactor AT heyazhou heatstresspromotestheaccumulationoftomatoyellowleafcurlvirusinitsinsectvectorbyactivatingheatshockfactor AT cuellarwilmerjose heatstresspromotestheaccumulationoftomatoyellowleafcurlvirusinitsinsectvectorbyactivatingheatshockfactor AT wangxiaowei heatstresspromotestheaccumulationoftomatoyellowleafcurlvirusinitsinsectvectorbyactivatingheatshockfactor |