Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava

The whitefly, Bemisia tabaci is a serious pest of cassava that causes yield loss through physical damage and vectoring of viruses that cause devastating cassava mosaic (CMD) and cassava brown streak (CBSD) diseases. The objective of this study was to evaluate the efficacy of commercially available e...

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Main Authors: Amour, M., Issa, K.A., Wosula, E.N., Ndalahwa, M., Stephano, F., Chandler, D., Ndyetabula, I., Legg, J.P.
Format: Journal Article
Language:Inglés
Published: Frontiers Media 2025
Subjects:
Online Access:https://hdl.handle.net/10568/175865
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author Amour, M.
Issa, K.A.
Wosula, E.N.
Ndalahwa, M.
Stephano, F.
Chandler, D.
Ndyetabula, I.
Legg, J.P.
author_browse Amour, M.
Chandler, D.
Issa, K.A.
Legg, J.P.
Ndalahwa, M.
Ndyetabula, I.
Stephano, F.
Wosula, E.N.
author_facet Amour, M.
Issa, K.A.
Wosula, E.N.
Ndalahwa, M.
Stephano, F.
Chandler, D.
Ndyetabula, I.
Legg, J.P.
author_sort Amour, M.
collection Repository of Agricultural Research Outputs (CGSpace)
description The whitefly, Bemisia tabaci is a serious pest of cassava that causes yield loss through physical damage and vectoring of viruses that cause devastating cassava mosaic (CMD) and cassava brown streak (CBSD) diseases. The objective of this study was to evaluate the efficacy of commercially available entomopathogenic fungi biopesticides (EPFs) and cutting dipping in insecticides alone and in combination with spraying EPFs against cassava B. tabaci. Laboratory experiments were conducted to test three commercial EPFs against cassava whitefly at IITA Tanzania. Data were recorded for nymph mortality. Field experiments were conducted at three Tanzania Agricultural Research Institute (TARI) stations (Chambezi, Mkuranga and Ukiruguru) to evaluate the efficacy of two EPFs, and the efficacy of cutting dipping in insecticides combined with spraying EPF against B. tabaci during the period of December 2021 to August 2023. The experimental design for field experiments was a randomized complete block design with four replicates. The data recorded for field experiments were on whitefly and nymph numbers, CMD and CBSD incidence and severity, and root yield. Mortality levels caused by EPFs under laboratory conditions were 35 – 86% at 14 days after application. In field experiments, EPFs reduced the proportion of healthy nymphs by 64 – 75% compared to the control, with no effect on adult whitefly numbers, CBSD and root yield. For cutting dips in insecticides at Chambezi, MandiPlus the most effective treatment reduced adult whiteflies by 85% and nymphs by 88%, CMD incidence by 59% and CBSD by 46% and increased stem number by 119% and root yield by 50%. These findings confirm that the application of MandiPlus through cutting dips is effective at reducing whitefly populations on cassava in Tanzania, reducing virus incidence and increasing yield. Application of entomopathogenic fungi under field conditions for control of cassava whitefly does not confer any significant benefits in terms of disease reduction and yield gain. Cutting dipping in insecticides is recommended for adoption as a component in IPM strategy for managing whiteflies and the viruses that they transmit.
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spelling CGSpace1758652025-12-08T10:29:22Z Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava Amour, M. Issa, K.A. Wosula, E.N. Ndalahwa, M. Stephano, F. Chandler, D. Ndyetabula, I. Legg, J.P. cassava bemisia tabaci entomopathogenic fungi insecticides cassava brown streak disease The whitefly, Bemisia tabaci is a serious pest of cassava that causes yield loss through physical damage and vectoring of viruses that cause devastating cassava mosaic (CMD) and cassava brown streak (CBSD) diseases. The objective of this study was to evaluate the efficacy of commercially available entomopathogenic fungi biopesticides (EPFs) and cutting dipping in insecticides alone and in combination with spraying EPFs against cassava B. tabaci. Laboratory experiments were conducted to test three commercial EPFs against cassava whitefly at IITA Tanzania. Data were recorded for nymph mortality. Field experiments were conducted at three Tanzania Agricultural Research Institute (TARI) stations (Chambezi, Mkuranga and Ukiruguru) to evaluate the efficacy of two EPFs, and the efficacy of cutting dipping in insecticides combined with spraying EPF against B. tabaci during the period of December 2021 to August 2023. The experimental design for field experiments was a randomized complete block design with four replicates. The data recorded for field experiments were on whitefly and nymph numbers, CMD and CBSD incidence and severity, and root yield. Mortality levels caused by EPFs under laboratory conditions were 35 – 86% at 14 days after application. In field experiments, EPFs reduced the proportion of healthy nymphs by 64 – 75% compared to the control, with no effect on adult whitefly numbers, CBSD and root yield. For cutting dips in insecticides at Chambezi, MandiPlus the most effective treatment reduced adult whiteflies by 85% and nymphs by 88%, CMD incidence by 59% and CBSD by 46% and increased stem number by 119% and root yield by 50%. These findings confirm that the application of MandiPlus through cutting dips is effective at reducing whitefly populations on cassava in Tanzania, reducing virus incidence and increasing yield. Application of entomopathogenic fungi under field conditions for control of cassava whitefly does not confer any significant benefits in terms of disease reduction and yield gain. Cutting dipping in insecticides is recommended for adoption as a component in IPM strategy for managing whiteflies and the viruses that they transmit. 2025-07-14 2025-07-29T13:11:04Z 2025-07-29T13:11:04Z Journal Article https://hdl.handle.net/10568/175865 en Open Access application/pdf Frontiers Media Amour, M., Issa, K. A., Wosula, E.N., Ndalahwa, M., Stephano, F., Chandler, D., ... & Legg, J.P. Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava. Frontiers in Agronomy, 7: 1623632, 1-15.
spellingShingle cassava
bemisia tabaci
entomopathogenic fungi
insecticides
cassava brown streak disease
Amour, M.
Issa, K.A.
Wosula, E.N.
Ndalahwa, M.
Stephano, F.
Chandler, D.
Ndyetabula, I.
Legg, J.P.
Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava
title Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava
title_full Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava
title_fullStr Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava
title_full_unstemmed Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava
title_short Stem-cutting dipping in insecticides and biopesticide application for the control of Bemisia tabaci whitefly in cassava
title_sort stem cutting dipping in insecticides and biopesticide application for the control of bemisia tabaci whitefly in cassava
topic cassava
bemisia tabaci
entomopathogenic fungi
insecticides
cassava brown streak disease
url https://hdl.handle.net/10568/175865
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