Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties
Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/175733 |
| _version_ | 1855518461088759808 |
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| author | Tiozon, Rhowell Navarro Ong, Glenn Vincent P. Sartagoda, Kristel June D. Duque, Sheba Mae M. Alseekh, Saleh Bonto, Aldrin P. Gempesaw, Shem Pratap, Vipin Reginio, Florencio C. Tengco, Jonina Marie J. Seagan, Christian Tolentino, Joel H. G. Santiago, Dennis Marvin O. Fernie, Alisdair R. Sreenivasulu, Nese |
| author_browse | Alseekh, Saleh Bonto, Aldrin P. Duque, Sheba Mae M. Fernie, Alisdair R. Gempesaw, Shem Ong, Glenn Vincent P. Pratap, Vipin Reginio, Florencio C. Santiago, Dennis Marvin O. Sartagoda, Kristel June D. Seagan, Christian Sreenivasulu, Nese Tengco, Jonina Marie J. Tiozon, Rhowell Navarro Tolentino, Joel H. G. |
| author_facet | Tiozon, Rhowell Navarro Ong, Glenn Vincent P. Sartagoda, Kristel June D. Duque, Sheba Mae M. Alseekh, Saleh Bonto, Aldrin P. Gempesaw, Shem Pratap, Vipin Reginio, Florencio C. Tengco, Jonina Marie J. Seagan, Christian Tolentino, Joel H. G. Santiago, Dennis Marvin O. Fernie, Alisdair R. Sreenivasulu, Nese |
| author_sort | Tiozon, Rhowell Navarro |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole grain rice samples were screened for their antioxidant components and capacity, leading to the identification of three superior cultivars: Balatinao variable purple rice, Ketan Hitam variable purple rice, and Kintuman red rice. Using response surface methodology, rice bran extracts from these cultivars were subjected to microencapsulation to stabilize the phytochemicals. Among the microencapsulated rice bran extracts (MRBEs), Ketan Hitam MRBE demonstrated significantly higher total phenolic content (TPC) and antioxidant capacity. This enhancement is likely due to the increased concentrations of isovitexin, luteolin 7-glucoside, and vitexin following microencapsulation. Furthermore, compared to non-encapsulated rice bran extracts, MRBEs exhibited significantly improved anticancer activity against HCT116 (colon) and A549 (lung) cancer cell lines (P < 0.05). Subsequent fractionation of the MRBE allowed for the identification of the most bioactive fractions, which contained metabolites effective against these cancer cell lines. In addition, in vitrobioaccessibility assays revealed a controlled release of 19 targeted phenolic compounds. This release profile was characterized by an initial increase during the gastric digestion phase, followed by a decrease in the intestinal phase. Notably, phenolic compounds such as chlorogenic acid, gallic acid, and vanillin were preserved across the three rice varieties after microencapsulation. These findings underscore the potential of MRBEs as functional food ingredients or supplements, offering improved bioaccessibility of phenolics, enhanced antioxidant properties, and promising anticancer activity. The results support the integration of rice bran extracts into the rice value chain, promoting their use in functional health applications. |
| format | Journal Article |
| id | CGSpace175733 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1757332025-12-23T01:44:18Z Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties Tiozon, Rhowell Navarro Ong, Glenn Vincent P. Sartagoda, Kristel June D. Duque, Sheba Mae M. Alseekh, Saleh Bonto, Aldrin P. Gempesaw, Shem Pratap, Vipin Reginio, Florencio C. Tengco, Jonina Marie J. Seagan, Christian Tolentino, Joel H. G. Santiago, Dennis Marvin O. Fernie, Alisdair R. Sreenivasulu, Nese rice bran brown rice antioxidants phenolic compounds functional foods phytochemicals bioactive compounds food processing food composition food quality metabolites cell lines food supplements Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole grain rice samples were screened for their antioxidant components and capacity, leading to the identification of three superior cultivars: Balatinao variable purple rice, Ketan Hitam variable purple rice, and Kintuman red rice. Using response surface methodology, rice bran extracts from these cultivars were subjected to microencapsulation to stabilize the phytochemicals. Among the microencapsulated rice bran extracts (MRBEs), Ketan Hitam MRBE demonstrated significantly higher total phenolic content (TPC) and antioxidant capacity. This enhancement is likely due to the increased concentrations of isovitexin, luteolin 7-glucoside, and vitexin following microencapsulation. Furthermore, compared to non-encapsulated rice bran extracts, MRBEs exhibited significantly improved anticancer activity against HCT116 (colon) and A549 (lung) cancer cell lines (P < 0.05). Subsequent fractionation of the MRBE allowed for the identification of the most bioactive fractions, which contained metabolites effective against these cancer cell lines. In addition, in vitrobioaccessibility assays revealed a controlled release of 19 targeted phenolic compounds. This release profile was characterized by an initial increase during the gastric digestion phase, followed by a decrease in the intestinal phase. Notably, phenolic compounds such as chlorogenic acid, gallic acid, and vanillin were preserved across the three rice varieties after microencapsulation. These findings underscore the potential of MRBEs as functional food ingredients or supplements, offering improved bioaccessibility of phenolics, enhanced antioxidant properties, and promising anticancer activity. The results support the integration of rice bran extracts into the rice value chain, promoting their use in functional health applications. 2025-06 2025-07-23T05:57:06Z 2025-07-23T05:57:06Z Journal Article https://hdl.handle.net/10568/175733 en Open Access application/pdf Elsevier Tiozon Jr, Rhowell Navarro, Glenn Vincent Ong, Kristel June D. Sartagoda, Sheba Mae M. Duque, Saleh Alseekh, Aldrin P. Bonto, Shem Gempesaw Jr et al. "Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties." Food Hydrocolloids for Health (2025): 100221. |
| spellingShingle | rice bran brown rice antioxidants phenolic compounds functional foods phytochemicals bioactive compounds food processing food composition food quality metabolites cell lines food supplements Tiozon, Rhowell Navarro Ong, Glenn Vincent P. Sartagoda, Kristel June D. Duque, Sheba Mae M. Alseekh, Saleh Bonto, Aldrin P. Gempesaw, Shem Pratap, Vipin Reginio, Florencio C. Tengco, Jonina Marie J. Seagan, Christian Tolentino, Joel H. G. Santiago, Dennis Marvin O. Fernie, Alisdair R. Sreenivasulu, Nese Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_full | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_fullStr | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_full_unstemmed | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_short | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_sort | optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| topic | rice bran brown rice antioxidants phenolic compounds functional foods phytochemicals bioactive compounds food processing food composition food quality metabolites cell lines food supplements |
| url | https://hdl.handle.net/10568/175733 |
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