Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries
Background Stable isotope techniques using 13C to assess vitamin A (VA) dietary sources, absorption, and total body VA stores (TBSs) require determination of baseline 13C abundance. 13C-natural abundance is approximately 1.1% total carbon, but varies with foods consumed, supplements taken, and food...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/175725 |
| _version_ | 1855542056276983808 |
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| author | Gannon, Bryan M. Sombié, Olivier O. Zeba, Augustin N. Nama, Gabriel Medoua Bekele, Tesfaye Hailu Woldeyohannes, Meseret van Stuijvenberg, Martha E. Dhansay, Muhammad A. Urio, Elisaphinate M. Kaliwile, Chisela Chileshe, Justin Kalungwana, Ng’Andwe Davis, Christopher R. Grahn, Michael Tanumihardjo, Sherry A. |
| author_browse | Bekele, Tesfaye Hailu Chileshe, Justin Davis, Christopher R. Dhansay, Muhammad A. Gannon, Bryan M. Grahn, Michael Kaliwile, Chisela Kalungwana, Ng’Andwe Nama, Gabriel Medoua Sombié, Olivier O. Tanumihardjo, Sherry A. Urio, Elisaphinate M. Woldeyohannes, Meseret Zeba, Augustin N. van Stuijvenberg, Martha E. |
| author_facet | Gannon, Bryan M. Sombié, Olivier O. Zeba, Augustin N. Nama, Gabriel Medoua Bekele, Tesfaye Hailu Woldeyohannes, Meseret van Stuijvenberg, Martha E. Dhansay, Muhammad A. Urio, Elisaphinate M. Kaliwile, Chisela Chileshe, Justin Kalungwana, Ng’Andwe Davis, Christopher R. Grahn, Michael Tanumihardjo, Sherry A. |
| author_sort | Gannon, Bryan M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background
Stable isotope techniques using 13C to assess vitamin A (VA) dietary sources, absorption, and total body VA stores (TBSs) require determination of baseline 13C abundance. 13C-natural abundance is approximately 1.1% total carbon, but varies with foods consumed, supplements taken, and food fortification with synthetic retinyl palmitate.
Objectives
We determined 13C variation from purified serum retinol and the resulting impact on TBSs using pooled data from preschool children in Burkina Faso, Cameroon, Ethiopia, South Africa, Tanzania, and Zambia and Zambian women.
Methods
Seven studies included children (n = 639; 56 ± 25 mo; 48% female) and one in women (n = 138; 29 ± 8.5 y). Serum retinol 13C-natural abundance was determined using GC-C-IRMS. TBSs were available in 7 studies that employed retinol isotope dilution (RID). Serum CRP and α1-acid-glycoprotein (AGP) were available from 6 studies in children. Multivariate mixed models assessed the impact of covariates on retinol 13C. Spearman correlations and Bland–Altman analysis compared serum and milk retinol 13C and evaluated the impact of using study- or global-retinol 13C estimates on calculated TBSs.
Results
13C-natural abundance (%, median [Q1, Q3]) differed among countries (low: Zambia, 1.0744 [1.0736, 1.0753]; high: South Africa, 1.0773 [1.0769, 1.0779]) and was associated with TBSs, CRP, and AGP in children and with TBSs in women. 13C-enrichment from serum and milk retinol were correlated (r = 0.52; P = 0.0001). RID in children and women using study and global estimates had low mean bias (range, −3.7% to 2.2%), but larger 95% limits of agreement (range, −23% to 37%).
Conclusions
13C-natural abundance is different among human cohorts in Africa. Collecting this information in subgroups is recommended for surveys using RID. When TBSs are needed on individuals in clinical applications, baseline 13C measures are important and should be measured in all enrolled subjects. |
| format | Journal Article |
| id | CGSpace175725 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1757252025-12-08T10:06:44Z Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries Gannon, Bryan M. Sombié, Olivier O. Zeba, Augustin N. Nama, Gabriel Medoua Bekele, Tesfaye Hailu Woldeyohannes, Meseret van Stuijvenberg, Martha E. Dhansay, Muhammad A. Urio, Elisaphinate M. Kaliwile, Chisela Chileshe, Justin Kalungwana, Ng’Andwe Davis, Christopher R. Grahn, Michael Tanumihardjo, Sherry A. retinol preschool children women isotope dilution method stable isotopes Background Stable isotope techniques using 13C to assess vitamin A (VA) dietary sources, absorption, and total body VA stores (TBSs) require determination of baseline 13C abundance. 13C-natural abundance is approximately 1.1% total carbon, but varies with foods consumed, supplements taken, and food fortification with synthetic retinyl palmitate. Objectives We determined 13C variation from purified serum retinol and the resulting impact on TBSs using pooled data from preschool children in Burkina Faso, Cameroon, Ethiopia, South Africa, Tanzania, and Zambia and Zambian women. Methods Seven studies included children (n = 639; 56 ± 25 mo; 48% female) and one in women (n = 138; 29 ± 8.5 y). Serum retinol 13C-natural abundance was determined using GC-C-IRMS. TBSs were available in 7 studies that employed retinol isotope dilution (RID). Serum CRP and α1-acid-glycoprotein (AGP) were available from 6 studies in children. Multivariate mixed models assessed the impact of covariates on retinol 13C. Spearman correlations and Bland–Altman analysis compared serum and milk retinol 13C and evaluated the impact of using study- or global-retinol 13C estimates on calculated TBSs. Results 13C-natural abundance (%, median [Q1, Q3]) differed among countries (low: Zambia, 1.0744 [1.0736, 1.0753]; high: South Africa, 1.0773 [1.0769, 1.0779]) and was associated with TBSs, CRP, and AGP in children and with TBSs in women. 13C-enrichment from serum and milk retinol were correlated (r = 0.52; P = 0.0001). RID in children and women using study and global estimates had low mean bias (range, −3.7% to 2.2%), but larger 95% limits of agreement (range, −23% to 37%). Conclusions 13C-natural abundance is different among human cohorts in Africa. Collecting this information in subgroups is recommended for surveys using RID. When TBSs are needed on individuals in clinical applications, baseline 13C measures are important and should be measured in all enrolled subjects. 2023-04 2025-07-22T17:11:41Z 2025-07-22T17:11:41Z Journal Article https://hdl.handle.net/10568/175725 en Open Access Elsevier Gannon, Bryan M.; Sombié, Olivier O.; Zeba, Augustin N.; Nama, Gabriel Medoua; Bekele, Tesfaye Hailu; et al. 2023. Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries. Journal of Nutrition 153(4): 949-957. https://doi.org/10.1016/j.tjnut.2023.02.002 |
| spellingShingle | retinol preschool children women isotope dilution method stable isotopes Gannon, Bryan M. Sombié, Olivier O. Zeba, Augustin N. Nama, Gabriel Medoua Bekele, Tesfaye Hailu Woldeyohannes, Meseret van Stuijvenberg, Martha E. Dhansay, Muhammad A. Urio, Elisaphinate M. Kaliwile, Chisela Chileshe, Justin Kalungwana, Ng’Andwe Davis, Christopher R. Grahn, Michael Tanumihardjo, Sherry A. Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries |
| title | Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries |
| title_full | Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries |
| title_fullStr | Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries |
| title_full_unstemmed | Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries |
| title_short | Comparison of total body vitamin A stores using individual versus population 13C-natural abundance of serum retinol in preschool children and women residing in 6 diverse African countries |
| title_sort | comparison of total body vitamin a stores using individual versus population 13c natural abundance of serum retinol in preschool children and women residing in 6 diverse african countries |
| topic | retinol preschool children women isotope dilution method stable isotopes |
| url | https://hdl.handle.net/10568/175725 |
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