Identification of candidate genes regulating drought tolerance in pearl millet
Pearl millet is an important crop of the arid and semi-arid ecologies to sustain food and fodder production. The greater tolerance to drought stress attracts us to examine its cellular and molecular mechanisms via functional genomics approaches to augment the grain yield. Here, we studied the drough...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/175630 |
| _version_ | 1855519345231265792 |
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| author | Chakraborty, Animikha Viswanath, Aswini Malipatil, Renuka Semalaiyappan, Janani Shah, Priya Ronanki, Swarna Rathoe, Abhishek Singh, Sumer Pal Govindaraj, Mahalingam Tonapi, Vilas A. Thirunavukkarasu, Nepolean |
| author_browse | Chakraborty, Animikha Govindaraj, Mahalingam Malipatil, Renuka Rathoe, Abhishek Ronanki, Swarna Semalaiyappan, Janani Shah, Priya Singh, Sumer Pal Thirunavukkarasu, Nepolean Tonapi, Vilas A. Viswanath, Aswini |
| author_facet | Chakraborty, Animikha Viswanath, Aswini Malipatil, Renuka Semalaiyappan, Janani Shah, Priya Ronanki, Swarna Rathoe, Abhishek Singh, Sumer Pal Govindaraj, Mahalingam Tonapi, Vilas A. Thirunavukkarasu, Nepolean |
| author_sort | Chakraborty, Animikha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pearl millet is an important crop of the arid and semi-arid ecologies to sustain food and fodder production. The greater tolerance to drought stress attracts us to examine its cellular and molecular mechanisms via functional genomics approaches to augment the grain yield. Here, we studied the drought response of 48 inbreds representing four different maturity groups at the flowering stage. A set of 74 drought-responsive genes were separated into five major phylogenic groups belonging to eight functional groups, namely ABA signaling, hormone signaling, ion and osmotic homeostasis, TF-mediated regulation, molecular adaptation, signal transduction, physiological adaptation, detoxification, which were comprehensively studied. Among the conserved motifs of the drought-responsive genes, the protein kinases and MYB domain proteins were the most conserved ones. Comparative in-silico analysis of the drought genes across millet crops showed foxtail millet had most orthologs with pearl millet. Of 698 haplotypes identified across millet crops, MyC2 and Myb4 had maximum haplotypes. The protein–protein interaction network identified ABI2, P5CS, CDPK, DREB, MYB, and CYP707A3 as major hub genes. The expression assay showed the presence of common as well as unique drought-responsive genes across maturity groups. Drought tolerant genotypes in respective maturity groups were identified from the expression pattern of genes. Among several gene families, ABA signaling, TFs, and signaling proteins were the prospective contributors to drought tolerance across maturity groups. The functionally validated genes could be used as promising candidates in backcross breeding, genomic selection, and gene-editing schemes in pearl millet and other millet crops to increase the yield in drought-prone arid and semi-arid ecologies. |
| format | Journal Article |
| id | CGSpace175630 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1756302025-07-17T17:49:02Z Identification of candidate genes regulating drought tolerance in pearl millet Chakraborty, Animikha Viswanath, Aswini Malipatil, Renuka Semalaiyappan, Janani Shah, Priya Ronanki, Swarna Rathoe, Abhishek Singh, Sumer Pal Govindaraj, Mahalingam Tonapi, Vilas A. Thirunavukkarasu, Nepolean pearl millet drought resistance functional genomics candidate genes agriculture Pearl millet is an important crop of the arid and semi-arid ecologies to sustain food and fodder production. The greater tolerance to drought stress attracts us to examine its cellular and molecular mechanisms via functional genomics approaches to augment the grain yield. Here, we studied the drought response of 48 inbreds representing four different maturity groups at the flowering stage. A set of 74 drought-responsive genes were separated into five major phylogenic groups belonging to eight functional groups, namely ABA signaling, hormone signaling, ion and osmotic homeostasis, TF-mediated regulation, molecular adaptation, signal transduction, physiological adaptation, detoxification, which were comprehensively studied. Among the conserved motifs of the drought-responsive genes, the protein kinases and MYB domain proteins were the most conserved ones. Comparative in-silico analysis of the drought genes across millet crops showed foxtail millet had most orthologs with pearl millet. Of 698 haplotypes identified across millet crops, MyC2 and Myb4 had maximum haplotypes. The protein–protein interaction network identified ABI2, P5CS, CDPK, DREB, MYB, and CYP707A3 as major hub genes. The expression assay showed the presence of common as well as unique drought-responsive genes across maturity groups. Drought tolerant genotypes in respective maturity groups were identified from the expression pattern of genes. Among several gene families, ABA signaling, TFs, and signaling proteins were the prospective contributors to drought tolerance across maturity groups. The functionally validated genes could be used as promising candidates in backcross breeding, genomic selection, and gene-editing schemes in pearl millet and other millet crops to increase the yield in drought-prone arid and semi-arid ecologies. 2022 2025-07-14T18:35:06Z 2025-07-14T18:35:06Z Journal Article https://hdl.handle.net/10568/175630 en Open Access MDPI Chakraborty, Animikha; Viswanath, Aswini; Malipatil, Renuka; Semalaiyappan, Janani; Shah, Priya; Ronanki, Swarna; Rathoe, Abhishek; Singh, Sumer Pal; Govindaraj, Mahalingam; Tonapi, Vilas A.; and Thirunavukkarasu, Nepolean. 2022. Identification of candidate genes regulating drought tolerance in pearl millet. International Journal of Molecular Sciences 23(13): 6907. https://doi.org/10.3390/ijms2313690713 |
| spellingShingle | pearl millet drought resistance functional genomics candidate genes agriculture Chakraborty, Animikha Viswanath, Aswini Malipatil, Renuka Semalaiyappan, Janani Shah, Priya Ronanki, Swarna Rathoe, Abhishek Singh, Sumer Pal Govindaraj, Mahalingam Tonapi, Vilas A. Thirunavukkarasu, Nepolean Identification of candidate genes regulating drought tolerance in pearl millet |
| title | Identification of candidate genes regulating drought tolerance in pearl millet |
| title_full | Identification of candidate genes regulating drought tolerance in pearl millet |
| title_fullStr | Identification of candidate genes regulating drought tolerance in pearl millet |
| title_full_unstemmed | Identification of candidate genes regulating drought tolerance in pearl millet |
| title_short | Identification of candidate genes regulating drought tolerance in pearl millet |
| title_sort | identification of candidate genes regulating drought tolerance in pearl millet |
| topic | pearl millet drought resistance functional genomics candidate genes agriculture |
| url | https://hdl.handle.net/10568/175630 |
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