Multi-year field evaluation of nicotianamine biofortified bread wheat
Conventional breeding efforts for iron (Fe) and zinc (Zn) biofortification of bread wheat (Triticum aestivum L.) have been hindered by a lack of genetic variation for these traits and a negative correlation between grain Fe and Zn concentrations and yield. We have employed genetic engineering to con...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/175580 |
| _version_ | 1855524711598915584 |
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| author | Beasley, Jesse T. Bonneau, Julien P. Moreno-Moyano, Laura T. Callahan, Damien L. Howell, Kate S. Tako, Elad Taylor, Julian Glahn, Raymond P. Appels, Rudi Johnson, Alexander A. T. |
| author_browse | Appels, Rudi Beasley, Jesse T. Bonneau, Julien P. Callahan, Damien L. Glahn, Raymond P. Howell, Kate S. Johnson, Alexander A. T. Moreno-Moyano, Laura T. Tako, Elad Taylor, Julian |
| author_facet | Beasley, Jesse T. Bonneau, Julien P. Moreno-Moyano, Laura T. Callahan, Damien L. Howell, Kate S. Tako, Elad Taylor, Julian Glahn, Raymond P. Appels, Rudi Johnson, Alexander A. T. |
| author_sort | Beasley, Jesse T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Conventional breeding efforts for iron (Fe) and zinc (Zn) biofortification of bread wheat (Triticum aestivum L.) have been hindered by a lack of genetic variation for these traits and a negative correlation between grain Fe and Zn concentrations and yield. We have employed genetic engineering to constitutively express (CE) the rice (Oryza sativa) nicotianamine synthase 2 (OsNAS2) gene and upregulate biosynthesis of two metal chelators – nicotianamine (NA) and 2′-deoxymugineic acid (DMA) – in bread wheat, resulting in increased Fe and Zn concentrations in wholemeal and white flour. Here we describe multi-location confined field trial (CFT) evaluation of a low-copy transgenic CE-OsNAS2 wheat event (CE-1) over 3 years and demonstrate higher concentrations of NA, DMA, Fe, and Zn in CE-1 wholemeal flour, white flour, and white bread and higher Fe bioavailability in CE-1 white flour relative to a null segregant (NS) control. Multi-environment models of agronomic and grain nutrition traits revealed a negative correlation between grain yield and grain Fe, Zn, and total protein concentrations, yet no correlation between grain yield and grain NA and DMA concentrations. White flour Fe bioavailability was positively correlated with white flour NA concentration, suggesting that NA-chelated Fe should be targeted in wheat Fe biofortification efforts. |
| format | Journal Article |
| id | CGSpace175580 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1755802025-07-17T17:51:30Z Multi-year field evaluation of nicotianamine biofortified bread wheat Beasley, Jesse T. Bonneau, Julien P. Moreno-Moyano, Laura T. Callahan, Damien L. Howell, Kate S. Tako, Elad Taylor, Julian Glahn, Raymond P. Appels, Rudi Johnson, Alexander A. T. biofortification iron zinc flours bread bioavailability soft wheat nutrition nutrition anaemia hunger Conventional breeding efforts for iron (Fe) and zinc (Zn) biofortification of bread wheat (Triticum aestivum L.) have been hindered by a lack of genetic variation for these traits and a negative correlation between grain Fe and Zn concentrations and yield. We have employed genetic engineering to constitutively express (CE) the rice (Oryza sativa) nicotianamine synthase 2 (OsNAS2) gene and upregulate biosynthesis of two metal chelators – nicotianamine (NA) and 2′-deoxymugineic acid (DMA) – in bread wheat, resulting in increased Fe and Zn concentrations in wholemeal and white flour. Here we describe multi-location confined field trial (CFT) evaluation of a low-copy transgenic CE-OsNAS2 wheat event (CE-1) over 3 years and demonstrate higher concentrations of NA, DMA, Fe, and Zn in CE-1 wholemeal flour, white flour, and white bread and higher Fe bioavailability in CE-1 white flour relative to a null segregant (NS) control. Multi-environment models of agronomic and grain nutrition traits revealed a negative correlation between grain yield and grain Fe, Zn, and total protein concentrations, yet no correlation between grain yield and grain NA and DMA concentrations. White flour Fe bioavailability was positively correlated with white flour NA concentration, suggesting that NA-chelated Fe should be targeted in wheat Fe biofortification efforts. 2022-03 2025-07-09T20:10:06Z 2025-07-09T20:10:06Z Journal Article https://hdl.handle.net/10568/175580 en Open Access Wiley Beasley, Jesse T.; Bonneau, Julien P.; Moreno-Moyano, Laura T.; Callahan, Damien L.; Howell, Kate S.; Tako, Elad; et al. 2022. Multi-year field evaluation of nicotianamine biofortified bread wheat. Plant Journal 109(5): 1168-1182. https://doi.org/10.1111/tpj.15623 |
| spellingShingle | biofortification iron zinc flours bread bioavailability soft wheat nutrition nutrition anaemia hunger Beasley, Jesse T. Bonneau, Julien P. Moreno-Moyano, Laura T. Callahan, Damien L. Howell, Kate S. Tako, Elad Taylor, Julian Glahn, Raymond P. Appels, Rudi Johnson, Alexander A. T. Multi-year field evaluation of nicotianamine biofortified bread wheat |
| title | Multi-year field evaluation of nicotianamine biofortified bread wheat |
| title_full | Multi-year field evaluation of nicotianamine biofortified bread wheat |
| title_fullStr | Multi-year field evaluation of nicotianamine biofortified bread wheat |
| title_full_unstemmed | Multi-year field evaluation of nicotianamine biofortified bread wheat |
| title_short | Multi-year field evaluation of nicotianamine biofortified bread wheat |
| title_sort | multi year field evaluation of nicotianamine biofortified bread wheat |
| topic | biofortification iron zinc flours bread bioavailability soft wheat nutrition nutrition anaemia hunger |
| url | https://hdl.handle.net/10568/175580 |
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