Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol

Background Zinc deficiency poses significant health risks, particularly in low-income settings. This study aims to evaluate the impact of agronomically zinc biofortified (fermented and non-fermented) and post-harvest wheat flour flatbread on zinc status and metabolic health in adolescents and adult...

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Autores principales: Das, Jai K., Padhani, Zahra Ali, Khan, Muhammad, Mirani, Mushtaq, Rizvi, Arjumand, Chauhadry, Imran Ahmed, Yasin, Rahima, Ismail, Tariq, Akhtar, Saeed, Begum, Kehkashan, Iqbal, Junaid, Humayun, Khadija, Naseem, Hamna Amir, Malik, Kauser Abdulla, Bhutta, Zulfiqar A.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2024
Materias:
Acceso en línea:https://hdl.handle.net/10568/175567
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author Das, Jai K.
Padhani, Zahra Ali
Khan, Muhammad
Mirani, Mushtaq
Rizvi, Arjumand
Chauhadry, Imran Ahmed
Yasin, Rahima
Ismail, Tariq
Akhtar, Saeed
Begum, Kehkashan
Iqbal, Junaid
Humayun, Khadija
Naseem, Hamna Amir
Malik, Kauser Abdulla
Bhutta, Zulfiqar A.
author_browse Akhtar, Saeed
Begum, Kehkashan
Bhutta, Zulfiqar A.
Chauhadry, Imran Ahmed
Das, Jai K.
Humayun, Khadija
Iqbal, Junaid
Ismail, Tariq
Khan, Muhammad
Malik, Kauser Abdulla
Mirani, Mushtaq
Naseem, Hamna Amir
Padhani, Zahra Ali
Rizvi, Arjumand
Yasin, Rahima
author_facet Das, Jai K.
Padhani, Zahra Ali
Khan, Muhammad
Mirani, Mushtaq
Rizvi, Arjumand
Chauhadry, Imran Ahmed
Yasin, Rahima
Ismail, Tariq
Akhtar, Saeed
Begum, Kehkashan
Iqbal, Junaid
Humayun, Khadija
Naseem, Hamna Amir
Malik, Kauser Abdulla
Bhutta, Zulfiqar A.
author_sort Das, Jai K.
collection Repository of Agricultural Research Outputs (CGSpace)
description Background Zinc deficiency poses significant health risks, particularly in low-income settings. This study aims to evaluate the impact of agronomically zinc biofortified (fermented and non-fermented) and post-harvest wheat flour flatbread on zinc status and metabolic health in adolescents and adult women in rural Pakistan. Methods A four-arm triple-blind randomized controlled trial will be conducted in a rural district of Paki stan. Participants (adolescents aged 10–19 and adult women aged 20–40) will be assigned to receive fermented or unfermented high zinc agronomically biofortified wheat flour flat bread, post-harvest zinc-fortified wheat flour flatbread, or low zinc conventional whole wheat flour flatbread. The meal would be served once a day, six days a week for six months. The study aims to enroll 1000 participants and will be analyzed based on the intention-to-treat principle. The trial is registered with number NCT06092515. Outcomes Primary outcomes will include serum zinc concentration and metabolic markers, while secondary outcomes include anthropometric measurements, blood pressure, and dietary intake. Conclusion This trial will provide valuable insights into the efficacy of agronomically zinc biofortified wheat flour in improving zinc status and metabolic health. Findings may inform public health strategies to combat zinc deficiency in resource-limited settings.
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spelling CGSpace1755672025-10-26T12:55:21Z Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol Das, Jai K. Padhani, Zahra Ali Khan, Muhammad Mirani, Mushtaq Rizvi, Arjumand Chauhadry, Imran Ahmed Yasin, Rahima Ismail, Tariq Akhtar, Saeed Begum, Kehkashan Iqbal, Junaid Humayun, Khadija Naseem, Hamna Amir Malik, Kauser Abdulla Bhutta, Zulfiqar A. zinc nutrient deficiencies biofortification wheat flour Background Zinc deficiency poses significant health risks, particularly in low-income settings. This study aims to evaluate the impact of agronomically zinc biofortified (fermented and non-fermented) and post-harvest wheat flour flatbread on zinc status and metabolic health in adolescents and adult women in rural Pakistan. Methods A four-arm triple-blind randomized controlled trial will be conducted in a rural district of Paki stan. Participants (adolescents aged 10–19 and adult women aged 20–40) will be assigned to receive fermented or unfermented high zinc agronomically biofortified wheat flour flat bread, post-harvest zinc-fortified wheat flour flatbread, or low zinc conventional whole wheat flour flatbread. The meal would be served once a day, six days a week for six months. The study aims to enroll 1000 participants and will be analyzed based on the intention-to-treat principle. The trial is registered with number NCT06092515. Outcomes Primary outcomes will include serum zinc concentration and metabolic markers, while secondary outcomes include anthropometric measurements, blood pressure, and dietary intake. Conclusion This trial will provide valuable insights into the efficacy of agronomically zinc biofortified wheat flour in improving zinc status and metabolic health. Findings may inform public health strategies to combat zinc deficiency in resource-limited settings. 2024-06 2025-07-09T17:40:00Z 2025-07-09T17:40:00Z Journal Article https://hdl.handle.net/10568/175567 en Open Access Das, Jai K.; Padhani, Zahra Ali; Khan, Muhammad; Mirani, Mushtaq; Rizvi, Arjumand; Chauhadry, Imran Ahmed; et al. 2024. Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol. PLoS ONE 19(6): e0304462. https://doi.org/10.1371/journal.pone.0304462
spellingShingle zinc
nutrient deficiencies
biofortification
wheat flour
Das, Jai K.
Padhani, Zahra Ali
Khan, Muhammad
Mirani, Mushtaq
Rizvi, Arjumand
Chauhadry, Imran Ahmed
Yasin, Rahima
Ismail, Tariq
Akhtar, Saeed
Begum, Kehkashan
Iqbal, Junaid
Humayun, Khadija
Naseem, Hamna Amir
Malik, Kauser Abdulla
Bhutta, Zulfiqar A.
Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol
title Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol
title_full Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol
title_fullStr Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol
title_full_unstemmed Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol
title_short Efficacy of Zinc Fortified and Fermented Wheat Flour (EZAFFAW): A randomized controlled trial protocol
title_sort efficacy of zinc fortified and fermented wheat flour ezaffaw a randomized controlled trial protocol
topic zinc
nutrient deficiencies
biofortification
wheat flour
url https://hdl.handle.net/10568/175567
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