Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response
The accumulation of the livestock-harming cyanogenic glucoside dhurrin in the vegetative tissues limits the use of sorghum as a major pasture crop. This study integrates transcriptomics and metabolomics data from the ICSV 93046, CSH 24-MF and ICSR 14001 genotypes, which differ in drought tolerance a...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2026
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/175549 |
| _version_ | 1855533580010127360 |
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| author | Katamreddy, S.C. Jose, J. Reddy, B.P. Sivasakthi, K. Sanivarapu, H. Dube, N. Gaddameedi, A. Prasad, Kodukula V.S.V. Yogendra, K. Govindaraj, M. Kholova, J. Choudhury, S.R. Kavi Kishor, P.B. Varijakshapanicker, Padmakumar Bhatnagar-Mathur, P. Are, A.K. Reddy, P.S. |
| author_browse | Are, A.K. Bhatnagar-Mathur, P. Choudhury, S.R. Dube, N. Gaddameedi, A. Govindaraj, M. Jose, J. Katamreddy, S.C. Kavi Kishor, P.B. Kholova, J. Prasad, Kodukula V.S.V. Reddy, B.P. Reddy, P.S. Sanivarapu, H. Sivasakthi, K. Varijakshapanicker, Padmakumar Yogendra, K. |
| author_facet | Katamreddy, S.C. Jose, J. Reddy, B.P. Sivasakthi, K. Sanivarapu, H. Dube, N. Gaddameedi, A. Prasad, Kodukula V.S.V. Yogendra, K. Govindaraj, M. Kholova, J. Choudhury, S.R. Kavi Kishor, P.B. Varijakshapanicker, Padmakumar Bhatnagar-Mathur, P. Are, A.K. Reddy, P.S. |
| author_sort | Katamreddy, S.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The accumulation of the livestock-harming cyanogenic glucoside dhurrin in the vegetative tissues limits the use of sorghum as a major pasture crop. This study integrates transcriptomics and metabolomics data from the ICSV 93046, CSH 24-MF and ICSR 14001 genotypes, which differ in drought tolerance and cyanide potential (HCNp), to understand the molecular processes of cyanogenesis under drought stress conditions. While ICSV 93046 showed drought adaptation and reduced HCNp, ICSR 14001 and CSH 24-MF exhibited decreased drought stress tolerance with HCN accumulation. The differentially expressed gene (DEG) data showed drought-related genes were significantly upregulated in ICSV 93046 but downregulated in ICSR 14001. KEGG pathway analysis revealed enriched dhurrin biosynthesis and cyanoamino acid metabolism genes, with higher expression in ICSR 14001 than in ICSV 93046. WGCNA analysis revealed that hub genes are involved in drought-induced signalling components, such as phospholipases (PLPs) and lipoxygenases (LOXs), which are implicated in membrane protection. In drought-sensitive genotypes, stress-induced membrane damages lead to the release of dhurrin into the cytoplasm, thus elevating HCN content and activating defence responses. Conversely, the drought-adapted genotype could mitigate HCN production by averting membrane injury, thereby effectively modulating the oxidative stress and preventing the release of dhurrin into the cytoplasm. |
| format | Journal Article |
| id | CGSpace175549 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1755492026-01-19T10:06:37Z Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response Katamreddy, S.C. Jose, J. Reddy, B.P. Sivasakthi, K. Sanivarapu, H. Dube, N. Gaddameedi, A. Prasad, Kodukula V.S.V. Yogendra, K. Govindaraj, M. Kholova, J. Choudhury, S.R. Kavi Kishor, P.B. Varijakshapanicker, Padmakumar Bhatnagar-Mathur, P. Are, A.K. Reddy, P.S. crops sorghum The accumulation of the livestock-harming cyanogenic glucoside dhurrin in the vegetative tissues limits the use of sorghum as a major pasture crop. This study integrates transcriptomics and metabolomics data from the ICSV 93046, CSH 24-MF and ICSR 14001 genotypes, which differ in drought tolerance and cyanide potential (HCNp), to understand the molecular processes of cyanogenesis under drought stress conditions. While ICSV 93046 showed drought adaptation and reduced HCNp, ICSR 14001 and CSH 24-MF exhibited decreased drought stress tolerance with HCN accumulation. The differentially expressed gene (DEG) data showed drought-related genes were significantly upregulated in ICSV 93046 but downregulated in ICSR 14001. KEGG pathway analysis revealed enriched dhurrin biosynthesis and cyanoamino acid metabolism genes, with higher expression in ICSR 14001 than in ICSV 93046. WGCNA analysis revealed that hub genes are involved in drought-induced signalling components, such as phospholipases (PLPs) and lipoxygenases (LOXs), which are implicated in membrane protection. In drought-sensitive genotypes, stress-induced membrane damages lead to the release of dhurrin into the cytoplasm, thus elevating HCN content and activating defence responses. Conversely, the drought-adapted genotype could mitigate HCN production by averting membrane injury, thereby effectively modulating the oxidative stress and preventing the release of dhurrin into the cytoplasm. 2026 2025-07-09T04:33:49Z 2025-07-09T04:33:49Z Journal Article https://hdl.handle.net/10568/175549 en Limited Access Wiley Katamreddy, S.C., Jose, J., Reddy, B.P., Sivasakthi, K., Sanivarapu, H., Dube, N., Gaddameedi, A., Prasad, K.V.S.V., Yogendra, K., Govindaraj, M., Kholova, J., Choudhury, S.R., Kavi Kishor, P.B., Varijakshapanicker, P., Bhatnagar-Mathur, P., Are, A.K. and Reddy, P.S. 2026. Drought-induced cyanogenesis in sorghum (<i>Sorghum bicolor</i> L.): Genotypic variation in dhurrin biosynthesis and stress response. Plant, Cell & Environment. |
| spellingShingle | crops sorghum Katamreddy, S.C. Jose, J. Reddy, B.P. Sivasakthi, K. Sanivarapu, H. Dube, N. Gaddameedi, A. Prasad, Kodukula V.S.V. Yogendra, K. Govindaraj, M. Kholova, J. Choudhury, S.R. Kavi Kishor, P.B. Varijakshapanicker, Padmakumar Bhatnagar-Mathur, P. Are, A.K. Reddy, P.S. Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response |
| title | Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response |
| title_full | Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response |
| title_fullStr | Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response |
| title_full_unstemmed | Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response |
| title_short | Drought-induced cyanogenesis in sorghum (Sorghum bicolor L.): Genotypic variation in dhurrin biosynthesis and stress response |
| title_sort | drought induced cyanogenesis in sorghum sorghum bicolor l genotypic variation in dhurrin biosynthesis and stress response |
| topic | crops sorghum |
| url | https://hdl.handle.net/10568/175549 |
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