Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)

Farmers and breeders recognize that multiple abiotic stresses occurring simultaneously are more harmful to crops than individual stress conditions. In this study, pedigree-based breeding was employed to develop multiple abiotic stress-tolerant rice breeding lines (MTU 1010/IRINMST-291), using string...

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Autores principales: Priyadarshi, Rahul, Yadav, Shailesh, Pettem, Mamatha, Venkateshwarlu, Challa, Pranesh, K. J., Singh, Vikas K., Kumar, Arvind
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/175440
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author Priyadarshi, Rahul
Yadav, Shailesh
Pettem, Mamatha
Venkateshwarlu, Challa
Pranesh, K. J.
Singh, Vikas K.
Kumar, Arvind
author_browse Kumar, Arvind
Pettem, Mamatha
Pranesh, K. J.
Priyadarshi, Rahul
Singh, Vikas K.
Venkateshwarlu, Challa
Yadav, Shailesh
author_facet Priyadarshi, Rahul
Yadav, Shailesh
Pettem, Mamatha
Venkateshwarlu, Challa
Pranesh, K. J.
Singh, Vikas K.
Kumar, Arvind
author_sort Priyadarshi, Rahul
collection Repository of Agricultural Research Outputs (CGSpace)
description Farmers and breeders recognize that multiple abiotic stresses occurring simultaneously are more harmful to crops than individual stress conditions. In this study, pedigree-based breeding was employed to develop multiple abiotic stress-tolerant rice breeding lines (MTU 1010/IRINMST-291), using stringent phenotypic selection for germination and seedling stage cold stress tolerance, reproductive stage drought stress, and reproductive stage high-temperature stress. Among the developed lines, IRINMST-007, IRINMST-338, IRINMST-418, and IRINMST-129 emerged as high-performing families under non-stress, drought, and high-temperature conditions during the reproductive stage. Additionally, IRINMST-007, IRINMST-418, and IRINMST-129 showed strong cold tolerance at the seedling stage and germination stage. These breeding lines have the potential to address food security challenges posed by abiotic stresses. A total of 110 parental polymorphic markers were used for single-marker analysis in the 180 selected F5 recombinant inbred lines. RM5344 were found associated with percent spikelet fertility at reproductive stage high-temperature stress with a phenotypic variance of 24%. RM5911 was found to be associated with grain yield at reproductive stage drought stress with a phenotypic variance of 11%. RM1019 was found associated with seedling growth under cold stress with a phenotypic variance of 18%. RM5344 was found associated with survival rate at seedling stage cold stress with a phenotypic variance of 19%. RM555 was found associated with vigor of germination at germination stage cold stress with a phenotypic variance of 18%. These markers can be used for molecular breeding programs to develop multi-stress tolerant rice varieties.
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spelling CGSpace1754402025-11-12T04:56:58Z Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.) Priyadarshi, Rahul Yadav, Shailesh Pettem, Mamatha Venkateshwarlu, Challa Pranesh, K. J. Singh, Vikas K. Kumar, Arvind oryza sativa germination reproductive stage drought stress cold stress genetic resistance plant breeding genetic markers marker-assisted selection Farmers and breeders recognize that multiple abiotic stresses occurring simultaneously are more harmful to crops than individual stress conditions. In this study, pedigree-based breeding was employed to develop multiple abiotic stress-tolerant rice breeding lines (MTU 1010/IRINMST-291), using stringent phenotypic selection for germination and seedling stage cold stress tolerance, reproductive stage drought stress, and reproductive stage high-temperature stress. Among the developed lines, IRINMST-007, IRINMST-338, IRINMST-418, and IRINMST-129 emerged as high-performing families under non-stress, drought, and high-temperature conditions during the reproductive stage. Additionally, IRINMST-007, IRINMST-418, and IRINMST-129 showed strong cold tolerance at the seedling stage and germination stage. These breeding lines have the potential to address food security challenges posed by abiotic stresses. A total of 110 parental polymorphic markers were used for single-marker analysis in the 180 selected F5 recombinant inbred lines. RM5344 were found associated with percent spikelet fertility at reproductive stage high-temperature stress with a phenotypic variance of 24%. RM5911 was found to be associated with grain yield at reproductive stage drought stress with a phenotypic variance of 11%. RM1019 was found associated with seedling growth under cold stress with a phenotypic variance of 18%. RM5344 was found associated with survival rate at seedling stage cold stress with a phenotypic variance of 19%. RM555 was found associated with vigor of germination at germination stage cold stress with a phenotypic variance of 18%. These markers can be used for molecular breeding programs to develop multi-stress tolerant rice varieties. 2025-06 2025-07-02T07:57:56Z 2025-07-02T07:57:56Z Journal Article https://hdl.handle.net/10568/175440 en Limited Access application/pdf Springer Priyadarshi, Rahul, Shailesh Yadav, Mamatha Pettem, Challa Venkateshwarlu, K. J. Pranesh, Vikas K. Singh, and Arvind Kumar. "Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)." Euphytica 221, no. 6 (2025): 1-15.
spellingShingle oryza sativa
germination
reproductive stage
drought stress
cold stress
genetic resistance
plant breeding
genetic markers
marker-assisted selection
Priyadarshi, Rahul
Yadav, Shailesh
Pettem, Mamatha
Venkateshwarlu, Challa
Pranesh, K. J.
Singh, Vikas K.
Kumar, Arvind
Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)
title Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)
title_full Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)
title_fullStr Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)
title_full_unstemmed Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)
title_short Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)
title_sort development of breeding lines with multiple stress tolerance to cold drought and high temperature stress in rice oryza sativa l
topic oryza sativa
germination
reproductive stage
drought stress
cold stress
genetic resistance
plant breeding
genetic markers
marker-assisted selection
url https://hdl.handle.net/10568/175440
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