QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice
Drought limits water availability and negatively impacts rice growth. One of the strategies to overcome drought stress is the genetic improvement of root traits by marker-assisted selection (MAS). Using the MAS strategy, pyramiding quantitative trait loci (QTLs) efficiently stacks multiple QTLs. To...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Japanese Society for Root Research
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/175437 |
| _version_ | 1855521074103451648 |
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| author | Iba, Momoha Centeno, C. Arloo Kitomi, Yuka Natividad, Mignon A. Quintana, Marinell R. Irie, Kenji Henry, Amelia Uga, Yusaku |
| author_browse | Centeno, C. Arloo Henry, Amelia Iba, Momoha Irie, Kenji Kitomi, Yuka Natividad, Mignon A. Quintana, Marinell R. Uga, Yusaku |
| author_facet | Iba, Momoha Centeno, C. Arloo Kitomi, Yuka Natividad, Mignon A. Quintana, Marinell R. Irie, Kenji Henry, Amelia Uga, Yusaku |
| author_sort | Iba, Momoha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought limits water availability and negatively impacts rice growth. One of the strategies to overcome drought stress is the genetic improvement of root traits by marker-assisted selection (MAS). Using the MAS strategy, pyramiding quantitative trait loci (QTLs) efficiently stacks multiple QTLs. To elucidate the effects of pyramiding root QTLs on shoot production under drought, we used near-isogenic lines (NILs) and pyramided lines (PYLs), introducing single or multiple QTLs derived from Kinandang Patong (upland rice) into IR64 (lowland rice). We selected one QTL from each root morphological trait: root growth angle (DRO1), root thickness (Sta1), root length (qRL6.1), and root volume (qFSR4). We evaluated the effects of pyramiding root QTLs on root system architecture (RSA). In the basket method and 3D imaging by X-ray computed tomography, all NIL and PYLs with DRO1 showed robustly steeper root growth angle. In a hydroponic culture assay, root length QTL qRL6.1 increased maximum root length. In a lysimeter experiment, root volume QTL qFSR4 increased root volume per stem. Next, we examined the NILs and PYLs for shoot biomass production under drought conditions in lysimeter and field experiments. Two lines (DRO1-NIL and Sta1+DRO1-PYL) had a higher shoot biomass production under drought than IR64. DRO1-NIL exhibited deeper RSAs and increased water uptake compared to IR64. Sta1+DRO1-PYL was not as deep as DRO1-NIL but had a higher normalized difference vegetation index, lower canopy temperature, and higher shoot biomass in the field experiment. In summary, DRO1 plays a critical role in RSA, and pyramiding Sta1 with DRO1increases shoot biomass during drought. This suggests the pyramiding of root QTLs could improve RSA and enhance biomass production under drought stress. |
| format | Journal Article |
| id | CGSpace175437 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Japanese Society for Root Research |
| publisherStr | Japanese Society for Root Research |
| record_format | dspace |
| spelling | CGSpace1754372025-11-12T04:56:34Z QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice Iba, Momoha Centeno, C. Arloo Kitomi, Yuka Natividad, Mignon A. Quintana, Marinell R. Irie, Kenji Henry, Amelia Uga, Yusaku oryza sativa root system shoot root architecture plant morphology plant growth drought resistance water stress quantitative trait loci biomass plant water relations Drought limits water availability and negatively impacts rice growth. One of the strategies to overcome drought stress is the genetic improvement of root traits by marker-assisted selection (MAS). Using the MAS strategy, pyramiding quantitative trait loci (QTLs) efficiently stacks multiple QTLs. To elucidate the effects of pyramiding root QTLs on shoot production under drought, we used near-isogenic lines (NILs) and pyramided lines (PYLs), introducing single or multiple QTLs derived from Kinandang Patong (upland rice) into IR64 (lowland rice). We selected one QTL from each root morphological trait: root growth angle (DRO1), root thickness (Sta1), root length (qRL6.1), and root volume (qFSR4). We evaluated the effects of pyramiding root QTLs on root system architecture (RSA). In the basket method and 3D imaging by X-ray computed tomography, all NIL and PYLs with DRO1 showed robustly steeper root growth angle. In a hydroponic culture assay, root length QTL qRL6.1 increased maximum root length. In a lysimeter experiment, root volume QTL qFSR4 increased root volume per stem. Next, we examined the NILs and PYLs for shoot biomass production under drought conditions in lysimeter and field experiments. Two lines (DRO1-NIL and Sta1+DRO1-PYL) had a higher shoot biomass production under drought than IR64. DRO1-NIL exhibited deeper RSAs and increased water uptake compared to IR64. Sta1+DRO1-PYL was not as deep as DRO1-NIL but had a higher normalized difference vegetation index, lower canopy temperature, and higher shoot biomass in the field experiment. In summary, DRO1 plays a critical role in RSA, and pyramiding Sta1 with DRO1increases shoot biomass during drought. This suggests the pyramiding of root QTLs could improve RSA and enhance biomass production under drought stress. 2025 2025-07-02T07:57:36Z 2025-07-02T07:57:36Z Journal Article https://hdl.handle.net/10568/175437 en Open Access application/pdf Japanese Society for Root Research Iba, Momoha, C. Arloo Centeno, Yuka Kitomi, Mignon A. Natividad, Marinell R. Quintana, Kenji Irie, Amelia Henry, and Yusaku Uga. "QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice." Plant Root 19 (2025): 13-29. |
| spellingShingle | oryza sativa root system shoot root architecture plant morphology plant growth drought resistance water stress quantitative trait loci biomass plant water relations Iba, Momoha Centeno, C. Arloo Kitomi, Yuka Natividad, Mignon A. Quintana, Marinell R. Irie, Kenji Henry, Amelia Uga, Yusaku QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice |
| title | QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice |
| title_full | QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice |
| title_fullStr | QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice |
| title_full_unstemmed | QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice |
| title_short | QTL pyramiding reveals a crucial role of DEEPER ROOTING 1 on root system architecture adapted to drought stress in rice |
| title_sort | qtl pyramiding reveals a crucial role of deeper rooting 1 on root system architecture adapted to drought stress in rice |
| topic | oryza sativa root system shoot root architecture plant morphology plant growth drought resistance water stress quantitative trait loci biomass plant water relations |
| url | https://hdl.handle.net/10568/175437 |
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