Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation

Methane is considered as a potent greenhouse gas, emitted from ruminants through enteric fermentation. Several methane mitigation strategies have been proposed and reported, however, role of aromatic herbs and their bioactive components on elimination of methane is still unclear. Mint (<i>Mentha ca...

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Main Authors: Ahmed, S., Wu, J., Liu, Z., Jiang, A., Pu, X., Zhou, C., Lukuyu, Ben A., Kang, J., Tan, Z.
Format: Journal Article
Language:Inglés
Published: Elsevier 2025
Subjects:
Online Access:https://hdl.handle.net/10568/175147
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author Ahmed, S.
Wu, J.
Liu, Z.
Jiang, A.
Pu, X.
Zhou, C.
Lukuyu, Ben A.
Kang, J.
Tan, Z.
author_browse Ahmed, S.
Jiang, A.
Kang, J.
Liu, Z.
Lukuyu, Ben A.
Pu, X.
Tan, Z.
Wu, J.
Zhou, C.
author_facet Ahmed, S.
Wu, J.
Liu, Z.
Jiang, A.
Pu, X.
Zhou, C.
Lukuyu, Ben A.
Kang, J.
Tan, Z.
author_sort Ahmed, S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Methane is considered as a potent greenhouse gas, emitted from ruminants through enteric fermentation. Several methane mitigation strategies have been proposed and reported, however, role of aromatic herbs and their bioactive components on elimination of methane is still unclear. Mint (<i>Mentha canadensis</i> L.), turmeric (<i>Curcuma longa</i> L.), eupatorium (<i>Eupatorium fortunei</i> Turcz.) and dandelion (<i>Taraxacum officinale</i>) were selected for two separate <i>in vitro</i> fermentation, at first as substrates and then additives with TMR (control) at the level of 1% and 3%, and assessed in terms of conventional nutrients, fermentation characteristics, metabolomics and microbial diversity. The experiment was conducted by a completely randomized block design, which included three runs with each treatment, whereas treatments are considered fixed effects and runs as a random effect. Turmeric contained more CP and less NDF, ADF showed higher IVDMD (g/kg), total VFA (mM) but produced less CH4 (ml/g) both as substrates or additives. Eupatorium produced more H2 (ml/g) and CH4 (ml/g), contained less propionate content (P<0.05). Flavone, succinate, 2-hydroxycinnamic acid were the common metabolites present in all four herbs. <i>Lachnospiraceae</i>, <i>Prevotellaceae</i>, <i>Succinivibrionaceae</i>, <i>Oscillospiraceae</i> and <i>Selenomonadaceae</i> were most dominant families. <i>Succinivibrionaceae</i> in turmeric-treated rumen fluid, used H2 to produce propionate, thus, amount of H2 available for methanogenesis is decreased, capacitated this herb to reduce more methane than other herbs. Overall, 3% inclusion of turmeric with TMR is best both in terms of dry matter degradation and methane mitigation potentiality.
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spelling CGSpace1751472025-10-26T12:55:29Z Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation Ahmed, S. Wu, J. Liu, Z. Jiang, A. Pu, X. Zhou, C. Lukuyu, Ben A. Kang, J. Tan, Z. animal feeding greenhouse gases herbaceous plants methane emission Methane is considered as a potent greenhouse gas, emitted from ruminants through enteric fermentation. Several methane mitigation strategies have been proposed and reported, however, role of aromatic herbs and their bioactive components on elimination of methane is still unclear. Mint (<i>Mentha canadensis</i> L.), turmeric (<i>Curcuma longa</i> L.), eupatorium (<i>Eupatorium fortunei</i> Turcz.) and dandelion (<i>Taraxacum officinale</i>) were selected for two separate <i>in vitro</i> fermentation, at first as substrates and then additives with TMR (control) at the level of 1% and 3%, and assessed in terms of conventional nutrients, fermentation characteristics, metabolomics and microbial diversity. The experiment was conducted by a completely randomized block design, which included three runs with each treatment, whereas treatments are considered fixed effects and runs as a random effect. Turmeric contained more CP and less NDF, ADF showed higher IVDMD (g/kg), total VFA (mM) but produced less CH4 (ml/g) both as substrates or additives. Eupatorium produced more H2 (ml/g) and CH4 (ml/g), contained less propionate content (P<0.05). Flavone, succinate, 2-hydroxycinnamic acid were the common metabolites present in all four herbs. <i>Lachnospiraceae</i>, <i>Prevotellaceae</i>, <i>Succinivibrionaceae</i>, <i>Oscillospiraceae</i> and <i>Selenomonadaceae</i> were most dominant families. <i>Succinivibrionaceae</i> in turmeric-treated rumen fluid, used H2 to produce propionate, thus, amount of H2 available for methanogenesis is decreased, capacitated this herb to reduce more methane than other herbs. Overall, 3% inclusion of turmeric with TMR is best both in terms of dry matter degradation and methane mitigation potentiality. 2025-10 2025-06-18T06:59:17Z 2025-06-18T06:59:17Z Journal Article https://hdl.handle.net/10568/175147 en Limited Access Elsevier Ahmed, S., Wu, J., Liu, Z., Jiang, A., Pu, X., Zhou, C., Lukuyu, B.A., Kang, J. and Tan, Z. 2025. Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through <i>in vitro</i> ruminal fermentation. Environmental Research 283: 122172.
spellingShingle animal feeding
greenhouse gases
herbaceous plants
methane emission
Ahmed, S.
Wu, J.
Liu, Z.
Jiang, A.
Pu, X.
Zhou, C.
Lukuyu, Ben A.
Kang, J.
Tan, Z.
Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
title Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
title_full Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
title_fullStr Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
title_full_unstemmed Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
title_short Nutriomics-specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
title_sort nutriomics specific chromatogram of aromatic herbs to mitigate methane emission through in vitro ruminal fermentation
topic animal feeding
greenhouse gases
herbaceous plants
methane emission
url https://hdl.handle.net/10568/175147
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