Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice

Grain quality is a crucial factor for rice consumers, and as such, it is a major focus for breeders. Phenotyping for grain quality traits can be a laborious and costly process. In this study, we evaluated the effectiveness of KASP-SNP markers in selecting for grain quality traits, including aroma, g...

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Autores principales: Asante, Maxwell Darko, Ofosu, Kirpal Agyemang, Frimpong, Felix, Alphonso, Deladem Kwami, Nartey, Elizabeth, Obeng, Agyei Elvis, Bam, Ralph K., Gamenyah, Daniel D., Ribeiro, Priscilla F., William, Harindra Manilal
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/174532
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author Asante, Maxwell Darko
Ofosu, Kirpal Agyemang
Frimpong, Felix
Alphonso, Deladem Kwami
Nartey, Elizabeth
Obeng, Agyei Elvis
Bam, Ralph K.
Gamenyah, Daniel D.
Ribeiro, Priscilla F.
William, Harindra Manilal
author_browse Alphonso, Deladem Kwami
Asante, Maxwell Darko
Bam, Ralph K.
Frimpong, Felix
Gamenyah, Daniel D.
Nartey, Elizabeth
Obeng, Agyei Elvis
Ofosu, Kirpal Agyemang
Ribeiro, Priscilla F.
William, Harindra Manilal
author_facet Asante, Maxwell Darko
Ofosu, Kirpal Agyemang
Frimpong, Felix
Alphonso, Deladem Kwami
Nartey, Elizabeth
Obeng, Agyei Elvis
Bam, Ralph K.
Gamenyah, Daniel D.
Ribeiro, Priscilla F.
William, Harindra Manilal
author_sort Asante, Maxwell Darko
collection Repository of Agricultural Research Outputs (CGSpace)
description Grain quality is a crucial factor for rice consumers, and as such, it is a major focus for breeders. Phenotyping for grain quality traits can be a laborious and costly process. In this study, we evaluated the effectiveness of KASP-SNP markers in selecting for grain quality traits, including aroma, gelatinization temperature, grain length, grain width, and grain length: width ratio. We phenotyped 300 diverse rice genotypes for these four traits and genotyped them using their respective KASP-SNP markers. A regression analysis of the phenotype on the genotype was performed. The markers associated with the fgr-1 and GS3 genes explained 97 % and 94 % of the phenotypic variation for aroma and grain length, respectively. Two SNP markers (snpOS00440 and snpOS00441) associated with the GW5/SW5 gene each explained 82 % of the phenotypic variation for grain width, which increased marginally to 85 % when combined. However, two markers, snpOS00036 and snpOS00450, for gelatinization temperature explained only 2 % and 25 % of the phenotypic variation, respectively, indicating that these markers were not efficient in predicting this trait. Our findings suggest that the markers associated with aroma (fgr-1), grain length (GS3), and grain width (GW5/SW5) are highly efficient and reliable for marker-assisted selection.
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spelling CGSpace1745322025-12-08T09:54:28Z Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice Asante, Maxwell Darko Ofosu, Kirpal Agyemang Frimpong, Felix Alphonso, Deladem Kwami Nartey, Elizabeth Obeng, Agyei Elvis Bam, Ralph K. Gamenyah, Daniel D. Ribeiro, Priscilla F. William, Harindra Manilal genes quantitative trait loci snp genotyping rice grain quality Grain quality is a crucial factor for rice consumers, and as such, it is a major focus for breeders. Phenotyping for grain quality traits can be a laborious and costly process. In this study, we evaluated the effectiveness of KASP-SNP markers in selecting for grain quality traits, including aroma, gelatinization temperature, grain length, grain width, and grain length: width ratio. We phenotyped 300 diverse rice genotypes for these four traits and genotyped them using their respective KASP-SNP markers. A regression analysis of the phenotype on the genotype was performed. The markers associated with the fgr-1 and GS3 genes explained 97 % and 94 % of the phenotypic variation for aroma and grain length, respectively. Two SNP markers (snpOS00440 and snpOS00441) associated with the GW5/SW5 gene each explained 82 % of the phenotypic variation for grain width, which increased marginally to 85 % when combined. However, two markers, snpOS00036 and snpOS00450, for gelatinization temperature explained only 2 % and 25 % of the phenotypic variation, respectively, indicating that these markers were not efficient in predicting this trait. Our findings suggest that the markers associated with aroma (fgr-1), grain length (GS3), and grain width (GW5/SW5) are highly efficient and reliable for marker-assisted selection. 2025-09 2025-05-13T07:40:02Z 2025-05-13T07:40:02Z Journal Article https://hdl.handle.net/10568/174532 en Limited Access Elsevier Asante, M.D., Ofosu, K.A., Frimpong, F., Alphonso, D.K., Nartey, E., Obeng, A.E., Bam, R.K., Gamenyah, D.D., Ribeiro, P.F. and Manilal, W. 2025. Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice. Plant Gene 43:100503.
spellingShingle genes
quantitative trait loci
snp genotyping
rice
grain
quality
Asante, Maxwell Darko
Ofosu, Kirpal Agyemang
Frimpong, Felix
Alphonso, Deladem Kwami
Nartey, Elizabeth
Obeng, Agyei Elvis
Bam, Ralph K.
Gamenyah, Daniel D.
Ribeiro, Priscilla F.
William, Harindra Manilal
Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice
title Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice
title_full Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice
title_fullStr Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice
title_full_unstemmed Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice
title_short Effectiveness of KASP-SNP markers in selecting for grain quality traits in rice
title_sort effectiveness of kasp snp markers in selecting for grain quality traits in rice
topic genes
quantitative trait loci
snp genotyping
rice
grain
quality
url https://hdl.handle.net/10568/174532
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