Sheep genetic diversity in Bhutan using microsatellite markers

Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population...

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Main Authors: Tashi, D., Wafula, P., Yoshio, Y., Shinji, S., Kenji, O., Hanotte, Olivier H., Bang-Zhong, L., Hideyuki, M., Han Jianlin
Format: Journal Article
Language:Inglés
Published: Wiley 2010
Subjects:
Online Access:https://hdl.handle.net/10568/1745
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author Tashi, D.
Wafula, P.
Yoshio, Y.
Shinji, S.
Kenji, O.
Hanotte, Olivier H.
Bang-Zhong, L.
Hideyuki, M.
Han Jianlin
author_browse Bang-Zhong, L.
Han Jianlin
Hanotte, Olivier H.
Hideyuki, M.
Kenji, O.
Shinji, S.
Tashi, D.
Wafula, P.
Yoshio, Y.
author_facet Tashi, D.
Wafula, P.
Yoshio, Y.
Shinji, S.
Kenji, O.
Hanotte, Olivier H.
Bang-Zhong, L.
Hideyuki, M.
Han Jianlin
author_sort Tashi, D.
collection Repository of Agricultural Research Outputs (CGSpace)
description Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.
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spelling CGSpace17452023-12-27T19:59:53Z Sheep genetic diversity in Bhutan using microsatellite markers Tashi, D. Wafula, P. Yoshio, Y. Shinji, S. Kenji, O. Hanotte, Olivier H. Bang-Zhong, L. Hideyuki, M. Han Jianlin sheep genetics Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations. 2010-04 2010-05-28T19:10:27Z 2010-05-28T19:10:27Z Journal Article https://hdl.handle.net/10568/1745 en Limited Access Wiley Tashi Dorji; Jianlin, H.; Wafula, P.; Yoshio Yamamoto; Shinji Sasazaki; Kenji Oyama; Hanotte, O.; Bang-Zhong Lin; Hideyuki Mannen. 2010. Sheep genetic diversity in Bhutan using microsatellite markers. Animal Science Journal 81(2):145-151.
spellingShingle sheep
genetics
Tashi, D.
Wafula, P.
Yoshio, Y.
Shinji, S.
Kenji, O.
Hanotte, Olivier H.
Bang-Zhong, L.
Hideyuki, M.
Han Jianlin
Sheep genetic diversity in Bhutan using microsatellite markers
title Sheep genetic diversity in Bhutan using microsatellite markers
title_full Sheep genetic diversity in Bhutan using microsatellite markers
title_fullStr Sheep genetic diversity in Bhutan using microsatellite markers
title_full_unstemmed Sheep genetic diversity in Bhutan using microsatellite markers
title_short Sheep genetic diversity in Bhutan using microsatellite markers
title_sort sheep genetic diversity in bhutan using microsatellite markers
topic sheep
genetics
url https://hdl.handle.net/10568/1745
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