Nontargeted metabolomics: PTFI Platform

The PTFI Nontargeted Metabolomics platform supports the characterization of the small molecule composition in food. This method uses solid phase extraction (SPE) to isolate small molecules for analysis by reverse phased liquid chromatography-high resolution mass spectrometry (LC-HRMS). The PTFI N...

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Autores principales: Watkins, Steve, Odenkirk, Melanie T, Prenni, Jessica, Brinkley, Sarah
Formato: Brief
Lenguaje:Inglés
Publicado: 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/174494
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author Watkins, Steve
Odenkirk, Melanie T
Prenni, Jessica
Brinkley, Sarah
author_browse Brinkley, Sarah
Odenkirk, Melanie T
Prenni, Jessica
Watkins, Steve
author_facet Watkins, Steve
Odenkirk, Melanie T
Prenni, Jessica
Brinkley, Sarah
author_sort Watkins, Steve
collection Repository of Agricultural Research Outputs (CGSpace)
description The PTFI Nontargeted Metabolomics platform supports the characterization of the small molecule composition in food. This method uses solid phase extraction (SPE) to isolate small molecules for analysis by reverse phased liquid chromatography-high resolution mass spectrometry (LC-HRMS). The PTFI Nontargeted Metabolomics platform is adapted from existing protocols used by researchers. What sets the PTFI method apart is the focus on standardization and the incorporation of a unique internal retention standard reagent which is a mixture of 33 compounds nonendogenous to food. When researchers around the world employ the same standardized protocol and utilize the same internal standard reagent the resulting data can be harmonized and becomes comparable – allowing us to build a scalable data resource.
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spelling CGSpace1744942025-11-05T11:12:22Z Nontargeted metabolomics: PTFI Platform Watkins, Steve Odenkirk, Melanie T Prenni, Jessica Brinkley, Sarah nutrition metabolomics food composition The PTFI Nontargeted Metabolomics platform supports the characterization of the small molecule composition in food. This method uses solid phase extraction (SPE) to isolate small molecules for analysis by reverse phased liquid chromatography-high resolution mass spectrometry (LC-HRMS). The PTFI Nontargeted Metabolomics platform is adapted from existing protocols used by researchers. What sets the PTFI method apart is the focus on standardization and the incorporation of a unique internal retention standard reagent which is a mixture of 33 compounds nonendogenous to food. When researchers around the world employ the same standardized protocol and utilize the same internal standard reagent the resulting data can be harmonized and becomes comparable – allowing us to build a scalable data resource. 2025-05 2025-05-09T07:21:00Z 2025-05-09T07:21:00Z Brief https://hdl.handle.net/10568/174494 en Open Access application/pdf Watkins, S.; Odenkirk, M.T.; Prenni, J.; Brinkley, S. (2025) Nontargeted metabolomics: PTFI Platform. 3 p.
spellingShingle nutrition
metabolomics
food composition
Watkins, Steve
Odenkirk, Melanie T
Prenni, Jessica
Brinkley, Sarah
Nontargeted metabolomics: PTFI Platform
title Nontargeted metabolomics: PTFI Platform
title_full Nontargeted metabolomics: PTFI Platform
title_fullStr Nontargeted metabolomics: PTFI Platform
title_full_unstemmed Nontargeted metabolomics: PTFI Platform
title_short Nontargeted metabolomics: PTFI Platform
title_sort nontargeted metabolomics ptfi platform
topic nutrition
metabolomics
food composition
url https://hdl.handle.net/10568/174494
work_keys_str_mv AT watkinssteve nontargetedmetabolomicsptfiplatform
AT odenkirkmelaniet nontargetedmetabolomicsptfiplatform
AT prennijessica nontargetedmetabolomicsptfiplatform
AT brinkleysarah nontargetedmetabolomicsptfiplatform