A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity
Plants utilize calcium as a signaling molecule to regulate innate immunity, including PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI), in addition to controlling growth and development1,2. Recent extensive research has highlighted that the activation of calcium ion (Ca2+) channel...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Preprint |
| Language: | Inglés |
| Published: |
Springer
2025
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/174458 |
| _version_ | 1855542076298493952 |
|---|---|
| author | Wu, Jun Liu, Jianbin Xiao, Gui Liu, Hai Liang, Yi Yi, Zhaofeng Bin, Bai Liang, Xiushuo Luo, Sheng Yang, Jie Xue, Shaowu Sun, Wenxian Zhou, Bo Yuan, Fang |
| author_browse | Bin, Bai Liang, Xiushuo Liang, Yi Liu, Hai Liu, Jianbin Luo, Sheng Sun, Wenxian Wu, Jun Xiao, Gui Xue, Shaowu Yang, Jie Yi, Zhaofeng Yuan, Fang Zhou, Bo |
| author_facet | Wu, Jun Liu, Jianbin Xiao, Gui Liu, Hai Liang, Yi Yi, Zhaofeng Bin, Bai Liang, Xiushuo Luo, Sheng Yang, Jie Xue, Shaowu Sun, Wenxian Zhou, Bo Yuan, Fang |
| author_sort | Wu, Jun |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Plants utilize calcium as a signaling molecule to regulate innate immunity, including PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI), in addition to controlling growth and development1,2. Recent extensive research has highlighted that the activation of calcium ion (Ca2+) channels during PTI and the formation of Ca2+ channels during ETI are crucial for plant immunity3. However, comprehension on how crops substantially augment immunity through the enhancement of Ca2+ channel activity remains limited. Here, we report a rice lesion mimic mutant, called etd1 (elicitors triggered cell death 1), which also triggers cell death formation upon the challenge of rice blast elicitors. The recessive gain-of-function gene etd1 encodes a hypermorphic haplotype of OsCNGC13 which contains a single amino acid substitution of glycine-to-glutamate at the position of 483rd amino acid. The etd1 forms a novel non-canonical rectifying Ca2+ channel that significantly enhances Ca2+ influx. We position that etd1-driven excessive Ca2+ influx disrupts cellular calcium homeostasis, thereby triggering pathogen-induced cell death and conferring robust and broad-spectrum immunity to the rice blast pathogen. |
| format | Preprint |
| id | CGSpace174458 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1744582025-12-08T10:29:22Z A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity Wu, Jun Liu, Jianbin Xiao, Gui Liu, Hai Liang, Yi Yi, Zhaofeng Bin, Bai Liang, Xiushuo Luo, Sheng Yang, Jie Xue, Shaowu Sun, Wenxian Zhou, Bo Yuan, Fang calcium cell death mutants plant diseases gene expression innate immunity ion channels homeostasis haplotypes genetic variation genetic resistance Plants utilize calcium as a signaling molecule to regulate innate immunity, including PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI), in addition to controlling growth and development1,2. Recent extensive research has highlighted that the activation of calcium ion (Ca2+) channels during PTI and the formation of Ca2+ channels during ETI are crucial for plant immunity3. However, comprehension on how crops substantially augment immunity through the enhancement of Ca2+ channel activity remains limited. Here, we report a rice lesion mimic mutant, called etd1 (elicitors triggered cell death 1), which also triggers cell death formation upon the challenge of rice blast elicitors. The recessive gain-of-function gene etd1 encodes a hypermorphic haplotype of OsCNGC13 which contains a single amino acid substitution of glycine-to-glutamate at the position of 483rd amino acid. The etd1 forms a novel non-canonical rectifying Ca2+ channel that significantly enhances Ca2+ influx. We position that etd1-driven excessive Ca2+ influx disrupts cellular calcium homeostasis, thereby triggering pathogen-induced cell death and conferring robust and broad-spectrum immunity to the rice blast pathogen. 2025-03-27 2025-05-07T09:16:48Z 2025-05-07T09:16:48Z Preprint https://hdl.handle.net/10568/174458 en Open Access application/pdf Springer Wu, Jun, Jianbin Liu, Gui Xiao, Hai Liu, Yi Liang, Zhaofeng Yi, Bai Bin et al. "A Novel Non-Canonical Rectifying Calcium Channel in Rice Triggers Cell Death Mediated Robust Immunity." [Pre-print](2025). |
| spellingShingle | calcium cell death mutants plant diseases gene expression innate immunity ion channels homeostasis haplotypes genetic variation genetic resistance Wu, Jun Liu, Jianbin Xiao, Gui Liu, Hai Liang, Yi Yi, Zhaofeng Bin, Bai Liang, Xiushuo Luo, Sheng Yang, Jie Xue, Shaowu Sun, Wenxian Zhou, Bo Yuan, Fang A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| title | A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| title_full | A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| title_fullStr | A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| title_full_unstemmed | A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| title_short | A novel non-canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| title_sort | novel non canonical rectifying calcium channel in rice triggers cell death mediated robust immunity |
| topic | calcium cell death mutants plant diseases gene expression innate immunity ion channels homeostasis haplotypes genetic variation genetic resistance |
| url | https://hdl.handle.net/10568/174458 |
| work_keys_str_mv | AT wujun anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liujianbin anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT xiaogui anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liuhai anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liangyi anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT yizhaofeng anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT binbai anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liangxiushuo anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT luosheng anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT yangjie anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT xueshaowu anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT sunwenxian anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT zhoubo anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT yuanfang anovelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT wujun novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liujianbin novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT xiaogui novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liuhai novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liangyi novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT yizhaofeng novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT binbai novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT liangxiushuo novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT luosheng novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT yangjie novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT xueshaowu novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT sunwenxian novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT zhoubo novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity AT yuanfang novelnoncanonicalrectifyingcalciumchannelinricetriggerscelldeathmediatedrobustimmunity |