Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia
Canine distemper virus (CDV) is endemic in Zambia, while peste-des-petits-ruminants virus (PPRV) has not been detected. This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (n = 29) was re-analysed with virus neutralisation assa...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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BioMed Central
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/174409 |
| _version_ | 1855519922949455872 |
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| author | Lysholm, Sara Logan, N. Lindahl, Johanna F. Berg, M. Johansson, E. Bergkvist, P.K. Dautu, G. Chazya, R. Willett, B.J. Munyeme, M. Wensman, J.J. |
| author_browse | Berg, M. Bergkvist, P.K. Chazya, R. Dautu, G. Johansson, E. Lindahl, Johanna F. Logan, N. Lysholm, Sara Munyeme, M. Wensman, J.J. Willett, B.J. |
| author_facet | Lysholm, Sara Logan, N. Lindahl, Johanna F. Berg, M. Johansson, E. Bergkvist, P.K. Dautu, G. Chazya, R. Willett, B.J. Munyeme, M. Wensman, J.J. |
| author_sort | Lysholm, Sara |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Canine distemper virus (CDV) is endemic in Zambia, while peste-des-petits-ruminants virus (PPRV) has not been detected. This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (n = 29) was re-analysed with virus neutralisation assays (VNA) for antibodies to PPRV and CDV due to cross-reactivity concerns. c-ELISA indicated 6.44% PPRV seroprevalence on farms and 3.80% at markets. While VNA detected no PPRV antibodies, 45% (13/29) tested positive for CDV, possibly suggesting cross-reactivity. Given the study’s limitations, further research is needed to determine whether PPRV is present in Zambia and assess potential cross-reactivity. |
| format | Journal Article |
| id | CGSpace174409 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | CGSpace1744092025-12-08T10:06:44Z Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia Lysholm, Sara Logan, N. Lindahl, Johanna F. Berg, M. Johansson, E. Bergkvist, P.K. Dautu, G. Chazya, R. Willett, B.J. Munyeme, M. Wensman, J.J. animal diseases canine distemper goats peste des petits ruminants sheep small ruminants Canine distemper virus (CDV) is endemic in Zambia, while peste-des-petits-ruminants virus (PPRV) has not been detected. This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (n = 29) was re-analysed with virus neutralisation assays (VNA) for antibodies to PPRV and CDV due to cross-reactivity concerns. c-ELISA indicated 6.44% PPRV seroprevalence on farms and 3.80% at markets. While VNA detected no PPRV antibodies, 45% (13/29) tested positive for CDV, possibly suggesting cross-reactivity. Given the study’s limitations, further research is needed to determine whether PPRV is present in Zambia and assess potential cross-reactivity. 2025-04-30 2025-05-02T06:34:03Z 2025-05-02T06:34:03Z Journal Article https://hdl.handle.net/10568/174409 en Open Access BioMed Central Lysholm, S., Logan, N., Lindahl, J.F., Berg, M., Johansson, E., Bergkvist, P.K., Dautu, G., Chazya, R., Willett, B.J., Munyeme, M. and Wensman, J.J. 2025. Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia. BMC Veterinary Research 21: 303. |
| spellingShingle | animal diseases canine distemper goats peste des petits ruminants sheep small ruminants Lysholm, Sara Logan, N. Lindahl, Johanna F. Berg, M. Johansson, E. Bergkvist, P.K. Dautu, G. Chazya, R. Willett, B.J. Munyeme, M. Wensman, J.J. Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia |
| title | Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia |
| title_full | Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia |
| title_fullStr | Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia |
| title_full_unstemmed | Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia |
| title_short | Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia |
| title_sort | detection of canine distemper virus cdv neutralising antibodies in small ruminants during peste des petits ruminants virus pprv surveillance in zambia |
| topic | animal diseases canine distemper goats peste des petits ruminants sheep small ruminants |
| url | https://hdl.handle.net/10568/174409 |
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