Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium
Cadmium (Cd) is a heavy metal which is toxic to both plants and animal. The high content of Cd in the rice grain severely threatens human's health. Here, we identified a Cd sensitive gene, named Cadmium Sensitive Gene 1 (OsCSG1), playing an important role in improving Cd tolerance in rice at seedlin...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/174395 |
| _version_ | 1855522618704134144 |
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| author | Liang, Shanshan Lan, Zhipeng Wang, Jiahan Zou, Wenli Hu, Youchuan Ran, Hongyu Qin, Mao Xiao, Gui Zhang, Siju Ma, Xuan Ye, Guoyou Luan, Weijiang |
| author_browse | Hu, Youchuan Lan, Zhipeng Liang, Shanshan Luan, Weijiang Ma, Xuan Qin, Mao Ran, Hongyu Wang, Jiahan Xiao, Gui Ye, Guoyou Zhang, Siju Zou, Wenli |
| author_facet | Liang, Shanshan Lan, Zhipeng Wang, Jiahan Zou, Wenli Hu, Youchuan Ran, Hongyu Qin, Mao Xiao, Gui Zhang, Siju Ma, Xuan Ye, Guoyou Luan, Weijiang |
| author_sort | Liang, Shanshan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cadmium (Cd) is a heavy metal which is toxic to both plants and animal. The high content of Cd in the rice grain severely threatens human's health. Here, we identified a Cd sensitive gene, named Cadmium Sensitive Gene 1 (OsCSG1), playing an important role in improving Cd tolerance in rice at seedling stage. The expression of OsCSG1 was induced by CdCl2 and exhibited higher mRNA levels in leaf blade, leaf sheath and stele of roots. Knockout of OsCSG1 improved the Cd tolerance of rice seedlings, suggesting that OsCSG1 negatively regulated Cd tolerance in rice. The Cd concentration in roots of seedling of oscsg1 mutants increased significantly under Cd stress, but not in the shoot and grains compared with wild type (WT). Subcellular distribution of Cd in root cells suggested that Cd proportions in soluble fractions of cells in oscsg1 mutant increased significantly. And CAT activity in oscsg1 mutants increased significantly. Taken together, knocking out OsCSG1 could improve Cd tolerance in rice by regulating subcellular distribution of cadmium and increased CAT activity. |
| format | Journal Article |
| id | CGSpace174395 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1743952025-12-08T10:06:44Z Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium Liang, Shanshan Lan, Zhipeng Wang, Jiahan Zou, Wenli Hu, Youchuan Ran, Hongyu Qin, Mao Xiao, Gui Zhang, Siju Ma, Xuan Ye, Guoyou Luan, Weijiang cadmium heavy metals toxicity gene expression mutants root system shoots grains abiotic stress rice Cadmium (Cd) is a heavy metal which is toxic to both plants and animal. The high content of Cd in the rice grain severely threatens human's health. Here, we identified a Cd sensitive gene, named Cadmium Sensitive Gene 1 (OsCSG1), playing an important role in improving Cd tolerance in rice at seedling stage. The expression of OsCSG1 was induced by CdCl2 and exhibited higher mRNA levels in leaf blade, leaf sheath and stele of roots. Knockout of OsCSG1 improved the Cd tolerance of rice seedlings, suggesting that OsCSG1 negatively regulated Cd tolerance in rice. The Cd concentration in roots of seedling of oscsg1 mutants increased significantly under Cd stress, but not in the shoot and grains compared with wild type (WT). Subcellular distribution of Cd in root cells suggested that Cd proportions in soluble fractions of cells in oscsg1 mutant increased significantly. And CAT activity in oscsg1 mutants increased significantly. Taken together, knocking out OsCSG1 could improve Cd tolerance in rice by regulating subcellular distribution of cadmium and increased CAT activity. 2025-05 2025-04-30T08:48:41Z 2025-04-30T08:48:41Z Journal Article https://hdl.handle.net/10568/174395 en Limited Access Elsevier Liang, Shanshan, Zhipeng Lan, Jiahan Wang, Wenli Zou, Youchuan Hu, Hongyu Ran, Mao Qin et al. "Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium." International Journal of Biological Macromolecules 306 (2025): 141604. |
| spellingShingle | cadmium heavy metals toxicity gene expression mutants root system shoots grains abiotic stress rice Liang, Shanshan Lan, Zhipeng Wang, Jiahan Zou, Wenli Hu, Youchuan Ran, Hongyu Qin, Mao Xiao, Gui Zhang, Siju Ma, Xuan Ye, Guoyou Luan, Weijiang Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium |
| title | Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium |
| title_full | Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium |
| title_fullStr | Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium |
| title_full_unstemmed | Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium |
| title_short | Knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice (Oryza sativa L.) by regulating the subcellular distribution of cadmium |
| title_sort | knockout of cadmium sensitive gene 1 confers enhanced cadmium tolerance in rice oryza sativa l by regulating the subcellular distribution of cadmium |
| topic | cadmium heavy metals toxicity gene expression mutants root system shoots grains abiotic stress rice |
| url | https://hdl.handle.net/10568/174395 |
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