Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD
Cassava is an important staple crop in tropical and subtropical regions. Cassava mosaic disease (CMD) is one of the most dangerous diseases affecting cassava production in Africa. Since the first reported in Southeast Asia in 2015, the CMD prevalence has become a concern in Southeast Asia. To comba...
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Japanese Society of Breeding
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/174313 |
| _version_ | 1855543108310138880 |
|---|---|
| author | Tokunaga, Hiroki Nhan, Pham Thi Huong, Pham Thi Anh, Nguyen Hai Huong, Le Thi Mai Hoa, Truong Minh Trang, Nguyen Thi Huyen Tung, Nguyen Ba Thuy, Cu Thi Le Zhang, Xiaofei Seki, Motoaki Ham, Le Huy |
| author_browse | Anh, Nguyen Hai Ham, Le Huy Hoa, Truong Minh Huong, Le Thi Mai Huong, Pham Thi Nhan, Pham Thi Seki, Motoaki Thuy, Cu Thi Le Tokunaga, Hiroki Trang, Nguyen Thi Huyen Tung, Nguyen Ba Zhang, Xiaofei |
| author_facet | Tokunaga, Hiroki Nhan, Pham Thi Huong, Pham Thi Anh, Nguyen Hai Huong, Le Thi Mai Hoa, Truong Minh Trang, Nguyen Thi Huyen Tung, Nguyen Ba Thuy, Cu Thi Le Zhang, Xiaofei Seki, Motoaki Ham, Le Huy |
| author_sort | Tokunaga, Hiroki |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava is an important staple crop in tropical and subtropical regions. Cassava mosaic disease (CMD) is one
of the most dangerous diseases affecting cassava production in Africa. Since the first reported in Southeast Asia in 2015, the CMD prevalence has become a concern in Southeast Asia. To combat it, CMD resistance has been introduced from African cassava into Asian elite cultivars. However, efficient DNA markers for the selection of CMD resistance are not available. The CMD2 locus confers resistance to African cassava mosaic virus via non-synonymous substitutions in the DNA polymerase δ subunit 1 gene (MePOLD1). Here, we developed DNA markers to identify the mutations providing the resistance. We examined the association between the resistance score in CMD-infected fields and the genotypes of hybrids of CMD-resistant and ‑susceptible Asian lines. Our study provides powerful tools to the global cassava breeding community for selecting CMD resistant cassava. |
| format | Journal Article |
| id | CGSpace174313 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Japanese Society of Breeding |
| publisherStr | Japanese Society of Breeding |
| record_format | dspace |
| spelling | CGSpace1743132025-12-08T09:54:28Z Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD Tokunaga, Hiroki Nhan, Pham Thi Huong, Pham Thi Anh, Nguyen Hai Huong, Le Thi Mai Hoa, Truong Minh Trang, Nguyen Thi Huyen Tung, Nguyen Ba Thuy, Cu Thi Le Zhang, Xiaofei Seki, Motoaki Ham, Le Huy cassava Cassava is an important staple crop in tropical and subtropical regions. Cassava mosaic disease (CMD) is one of the most dangerous diseases affecting cassava production in Africa. Since the first reported in Southeast Asia in 2015, the CMD prevalence has become a concern in Southeast Asia. To combat it, CMD resistance has been introduced from African cassava into Asian elite cultivars. However, efficient DNA markers for the selection of CMD resistance are not available. The CMD2 locus confers resistance to African cassava mosaic virus via non-synonymous substitutions in the DNA polymerase δ subunit 1 gene (MePOLD1). Here, we developed DNA markers to identify the mutations providing the resistance. We examined the association between the resistance score in CMD-infected fields and the genotypes of hybrids of CMD-resistant and ‑susceptible Asian lines. Our study provides powerful tools to the global cassava breeding community for selecting CMD resistant cassava. 2025 2025-04-24T08:14:11Z 2025-04-24T08:14:11Z Journal Article https://hdl.handle.net/10568/174313 en Open Access application/pdf Japanese Society of Breeding Tokunaga, H.; Nhan, P.T.; Huong, P.T.; Anh, N.H.; Huong, L.T.M.; Hoa, T.M.; Trang, N.T.H.; Tung, N.B.; Thuy, C.T.L.; Zhang, X.; Seki, M.; Ham, L.H. (2025) Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD. Breeding Science 75(2): ISSN: 1344-7610 |
| spellingShingle | cassava Tokunaga, Hiroki Nhan, Pham Thi Huong, Pham Thi Anh, Nguyen Hai Huong, Le Thi Mai Hoa, Truong Minh Trang, Nguyen Thi Huyen Tung, Nguyen Ba Thuy, Cu Thi Le Zhang, Xiaofei Seki, Motoaki Ham, Le Huy Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD |
| title | Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD |
| title_full | Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD |
| title_fullStr | Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD |
| title_full_unstemmed | Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD |
| title_short | Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease CMD |
| title_sort | development of dna markers for assisted selection of cassava resistant to cassava mosaic disease cmd |
| topic | cassava |
| url | https://hdl.handle.net/10568/174313 |
| work_keys_str_mv | AT tokunagahiroki developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT nhanphamthi developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT huongphamthi developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT anhnguyenhai developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT huonglethimai developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT hoatruongminh developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT trangnguyenthihuyen developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT tungnguyenba developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT thuycuthile developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT zhangxiaofei developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT sekimotoaki developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd AT hamlehuy developmentofdnamarkersforassistedselectionofcassavaresistanttocassavamosaicdiseasecmd |