Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas
Sentinel cassava plants were exposed to high disease pressure in the field to promote root symptom expression. After visual inspection, symptomatic plants were propagated in a screenhouse for a second growth cycle. Molecular analyses, including RT-PCR and HTS, were conducted to identify associated p...
| Main Authors: | , , , , , , |
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| Format: | Conjunto de datos |
| Language: | Inglés |
| Published: |
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/174282 |
| _version_ | 1855524275307413504 |
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| author | Cuellar, Wilmer Jose Jimenez Polo, Jenyfer Pardo Garcia, Juan Manuel Gil Ordoñez, Alejandra Caicedo Hidalgo, Sara Maria Alvarez Quinto, Robert Alexander Dimitre, Mollov |
| author_browse | Alvarez Quinto, Robert Alexander Caicedo Hidalgo, Sara Maria Cuellar, Wilmer Jose Dimitre, Mollov Gil Ordoñez, Alejandra Jimenez Polo, Jenyfer Pardo Garcia, Juan Manuel |
| author_facet | Cuellar, Wilmer Jose Jimenez Polo, Jenyfer Pardo Garcia, Juan Manuel Gil Ordoñez, Alejandra Caicedo Hidalgo, Sara Maria Alvarez Quinto, Robert Alexander Dimitre, Mollov |
| author_sort | Cuellar, Wilmer Jose |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sentinel cassava plants were exposed to high disease pressure in the field to promote root symptom expression. After visual inspection, symptomatic plants were propagated in a screenhouse for a second growth cycle. Molecular analyses, including RT-PCR and HTS, were conducted to identify associated pathogens and investigate their link to cassava frogskin symptoms.
Methodology: Sentinel plants were exposed to high disease pressure for one crop cycle to allow the development of root symptoms. At the end of the exposure period, a visual assessment was conducted to detect cassava frogskin symptoms. Selected plants were propagated and maintained under controlled conditions in a screenhouse for a second growth cycle, allowing the development of storage roots. Collected samples were subjected to molecular analyses, including targeted RT-PCR and HTS, to identify the presence of different pathogens and perform association studies with the observed symptoms. |
| format | Conjunto de datos |
| id | CGSpace174282 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| record_format | dspace |
| spelling | CGSpace1742822025-04-22T15:24:26Z Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas Cuellar, Wilmer Jose Jimenez Polo, Jenyfer Pardo Garcia, Juan Manuel Gil Ordoñez, Alejandra Caicedo Hidalgo, Sara Maria Alvarez Quinto, Robert Alexander Dimitre, Mollov diagnosis Sentinel cassava plants were exposed to high disease pressure in the field to promote root symptom expression. After visual inspection, symptomatic plants were propagated in a screenhouse for a second growth cycle. Molecular analyses, including RT-PCR and HTS, were conducted to identify associated pathogens and investigate their link to cassava frogskin symptoms. Methodology: Sentinel plants were exposed to high disease pressure for one crop cycle to allow the development of root symptoms. At the end of the exposure period, a visual assessment was conducted to detect cassava frogskin symptoms. Selected plants were propagated and maintained under controlled conditions in a screenhouse for a second growth cycle, allowing the development of storage roots. Collected samples were subjected to molecular analyses, including targeted RT-PCR and HTS, to identify the presence of different pathogens and perform association studies with the observed symptoms. 2025 2025-04-22T15:24:25Z 2025-04-22T15:24:25Z Dataset https://hdl.handle.net/10568/174282 en Open Access Cuellar, W.J.; Jimenez Polo, J.; Pardo Garcia, J.M.; Gil Ordoñez, A.; Caicedo Hidalgo, S.M.; Alvarez Quinto, R.A.; Dimitre, M. (2025) Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas. https://doi.org/10.7910/DVN/HFVOA8 |
| spellingShingle | diagnosis Cuellar, Wilmer Jose Jimenez Polo, Jenyfer Pardo Garcia, Juan Manuel Gil Ordoñez, Alejandra Caicedo Hidalgo, Sara Maria Alvarez Quinto, Robert Alexander Dimitre, Mollov Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title | Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_full | Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_fullStr | Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_full_unstemmed | Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_short | Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_sort | replication for single torradovirus infections explain the mysterious cassava frogskin disease in the americas |
| topic | diagnosis |
| url | https://hdl.handle.net/10568/174282 |
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