Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia

Diarrheal illness remains a major global health challenge, causing millions of deaths annually. Non-typhoidal <i>Salmonella</i> (NTS), Shiga toxin-producing <i>Escherichia coli</i> (STEC), and <i>Campylobacter</i> species (CAMPY) significantly contribute to this burden. Given the limited information...

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Autores principales: Teshale, A.M., Abegaz, W.E., Azmeraye, B.M., Degefaw, D., LaPolt, D., Bonger, Z., Kalayu, A.A., Tigabu, E., Gazu, Lina, Yimer, G., Abate, E., Tsige, E., Tasew, G., Dessie, Y., Biks, G., Barkley, J.A., Garsow, A.V., Beckiewicz, A., Alonso, Silvia, Kowalcyk, B.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/174277
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author Teshale, A.M.
Abegaz, W.E.
Azmeraye, B.M.
Degefaw, D.
LaPolt, D.
Bonger, Z.
Kalayu, A.A.
Tigabu, E.
Gazu, Lina
Yimer, G.
Abate, E.
Tsige, E.
Tasew, G.
Dessie, Y.
Biks, G.
Barkley, J.A.
Garsow, A.V.
Beckiewicz, A.
Alonso, Silvia
Kowalcyk, B.
author_browse Abate, E.
Abegaz, W.E.
Alonso, Silvia
Azmeraye, B.M.
Barkley, J.A.
Beckiewicz, A.
Biks, G.
Bonger, Z.
Degefaw, D.
Dessie, Y.
Garsow, A.V.
Gazu, Lina
Kalayu, A.A.
Kowalcyk, B.
LaPolt, D.
Tasew, G.
Teshale, A.M.
Tigabu, E.
Tsige, E.
Yimer, G.
author_facet Teshale, A.M.
Abegaz, W.E.
Azmeraye, B.M.
Degefaw, D.
LaPolt, D.
Bonger, Z.
Kalayu, A.A.
Tigabu, E.
Gazu, Lina
Yimer, G.
Abate, E.
Tsige, E.
Tasew, G.
Dessie, Y.
Biks, G.
Barkley, J.A.
Garsow, A.V.
Beckiewicz, A.
Alonso, Silvia
Kowalcyk, B.
author_sort Teshale, A.M.
collection Repository of Agricultural Research Outputs (CGSpace)
description Diarrheal illness remains a major global health challenge, causing millions of deaths annually. Non-typhoidal <i>Salmonella</i> (NTS), Shiga toxin-producing <i>Escherichia coli</i> (STEC), and <i>Campylobacter</i> species (CAMPY) significantly contribute to this burden. Given the limited information on these pathogens in Ethiopia, this study aimed to estimate their prevalence among diarrhea patients in Ethiopia and identify risk factors for infection. A cross-sectional study was conducted from October 2021 to November 2022 in three hospitals in Ethiopia (Addis Ababa, Gondar, and Harar). Sociodemographic characteristics, clinical signs and symptoms were collected from study participants using a structured questionnaire. Stool samples were tested for NTS, STEC, and CAMPY using standardized methods. The prevalence of targeted pathogens was estimated overall and by study sites. Univariable and multivariable logistic regression were used to identify associated factors. A total of 2,331 patients were enrolled. The overall prevalence of NTS, STEC (stx only), STEC (stx + eae), and CAMPY was 1.29% (95%CI: 0.91, 1.84), 12.56% (95%CI: 11.29, 13.98), 3.43% (95%CI: 2.77, 4.25), and 4.46% (95%CI: 4.61, 8.00), respectively. Harar had the highest prevalence of all the pathogens compared to Addis Ababa and Gondar. Odds of NTS in Harar were over 10 (AOR: 10.43: 95%CI: 2.95, 69.20) and 3.5 times (AOR: 3.57: 95%CI: 1.50, 9.90) higher than that in Addis Ababa and Gondar, respectively. Odds of STEC (stx only) in the dry (AOR: 1.97: 95%CI: 1.37, 2.90) and long rainy (AOR: 1.80: 95%CI: 1.20, 2.69) seasons were nearly twice the odds in the short rainy season. Odds of CAMPY infection decreased by 3.29% (AOR: 0.97: 95%CI: 0.95, 0.98) with every one-year increase in age. Moreover, the odds of CAMPY infection for rural residents (AOR: 1.93, 95%CI: 1.15, 3.19) were nearly twice that of urban residents. This is the first study to estimate the prevalence of NTS, STEC, and CAMPY simultaneously across all age groups and diverse regions in Ethiopia, revealing significant variations. Results can be used to understand the burden of disease, inform clinical management and risk mitigation strategies to reduce illness in Ethiopia.
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spelling CGSpace1742772025-10-26T12:54:54Z Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia Teshale, A.M. Abegaz, W.E. Azmeraye, B.M. Degefaw, D. LaPolt, D. Bonger, Z. Kalayu, A.A. Tigabu, E. Gazu, Lina Yimer, G. Abate, E. Tsige, E. Tasew, G. Dessie, Y. Biks, G. Barkley, J.A. Garsow, A.V. Beckiewicz, A. Alonso, Silvia Kowalcyk, B. health Diarrheal illness remains a major global health challenge, causing millions of deaths annually. Non-typhoidal <i>Salmonella</i> (NTS), Shiga toxin-producing <i>Escherichia coli</i> (STEC), and <i>Campylobacter</i> species (CAMPY) significantly contribute to this burden. Given the limited information on these pathogens in Ethiopia, this study aimed to estimate their prevalence among diarrhea patients in Ethiopia and identify risk factors for infection. A cross-sectional study was conducted from October 2021 to November 2022 in three hospitals in Ethiopia (Addis Ababa, Gondar, and Harar). Sociodemographic characteristics, clinical signs and symptoms were collected from study participants using a structured questionnaire. Stool samples were tested for NTS, STEC, and CAMPY using standardized methods. The prevalence of targeted pathogens was estimated overall and by study sites. Univariable and multivariable logistic regression were used to identify associated factors. A total of 2,331 patients were enrolled. The overall prevalence of NTS, STEC (stx only), STEC (stx + eae), and CAMPY was 1.29% (95%CI: 0.91, 1.84), 12.56% (95%CI: 11.29, 13.98), 3.43% (95%CI: 2.77, 4.25), and 4.46% (95%CI: 4.61, 8.00), respectively. Harar had the highest prevalence of all the pathogens compared to Addis Ababa and Gondar. Odds of NTS in Harar were over 10 (AOR: 10.43: 95%CI: 2.95, 69.20) and 3.5 times (AOR: 3.57: 95%CI: 1.50, 9.90) higher than that in Addis Ababa and Gondar, respectively. Odds of STEC (stx only) in the dry (AOR: 1.97: 95%CI: 1.37, 2.90) and long rainy (AOR: 1.80: 95%CI: 1.20, 2.69) seasons were nearly twice the odds in the short rainy season. Odds of CAMPY infection decreased by 3.29% (AOR: 0.97: 95%CI: 0.95, 0.98) with every one-year increase in age. Moreover, the odds of CAMPY infection for rural residents (AOR: 1.93, 95%CI: 1.15, 3.19) were nearly twice that of urban residents. This is the first study to estimate the prevalence of NTS, STEC, and CAMPY simultaneously across all age groups and diverse regions in Ethiopia, revealing significant variations. Results can be used to understand the burden of disease, inform clinical management and risk mitigation strategies to reduce illness in Ethiopia. 2025-04-21 2025-04-22T11:19:33Z 2025-04-22T11:19:33Z Journal Article https://hdl.handle.net/10568/174277 en Open Access Teshale, A.M., Abegaz, W.E., Azmeraye, B.M., Degefaw, D., LaPolt, D., Bonger, Z., Kalayu, A.A., Tigabu, E., Gazu, L., Yimer, G., Abate, E., Tsige, E., Tasew, G., Dessie, Y., Biks, G., Barkley, J.A., Garsow, A.V., Beckiewicz, A., Alonso, S. and Kowalcyk, B. 2025. Prevalence of Shiga toxin-producing <i>Escherichia coli</i>, <i>Salmonella</i>, and <i>Campylobacter</i> species among diarrheal patients from three major hospitals in Ethiopia. PLOS Global Public Health 5(4): e0004407.
spellingShingle health
Teshale, A.M.
Abegaz, W.E.
Azmeraye, B.M.
Degefaw, D.
LaPolt, D.
Bonger, Z.
Kalayu, A.A.
Tigabu, E.
Gazu, Lina
Yimer, G.
Abate, E.
Tsige, E.
Tasew, G.
Dessie, Y.
Biks, G.
Barkley, J.A.
Garsow, A.V.
Beckiewicz, A.
Alonso, Silvia
Kowalcyk, B.
Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia
title Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia
title_full Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia
title_fullStr Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia
title_full_unstemmed Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia
title_short Prevalence of Shiga toxin-producing Escherichia coli, Salmonella, and Campylobacter species among diarrheal patients from three major hospitals in Ethiopia
title_sort prevalence of shiga toxin producing escherichia coli salmonella and campylobacter species among diarrheal patients from three major hospitals in ethiopia
topic health
url https://hdl.handle.net/10568/174277
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