Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure
Rice hoja blanca virus (RHBV), transmitted by the insect vector Tagosodes orizicolus, poses a significant threat to rice cultivation. Here, we use CRISPR/ Cas9 technology to produce specific mutations in the AGO4 gene of Oryza sativa, using the Fedearroz 2000 variety, with the aim of elucidating the...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2025
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| Acceso en línea: | https://hdl.handle.net/10568/174109 |
| _version_ | 1855523568751738880 |
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| author | Nanez, Jhojan Valdes, Sandra Cruz Gallego, Maribel Rebolledo, Maria Camila Lorieux, Mathias Alvarez, Maria Fernanda Chavarriaga, Paul |
| author_browse | Alvarez, Maria Fernanda Chavarriaga, Paul Cruz Gallego, Maribel Lorieux, Mathias Nanez, Jhojan Rebolledo, Maria Camila Valdes, Sandra |
| author_facet | Nanez, Jhojan Valdes, Sandra Cruz Gallego, Maribel Rebolledo, Maria Camila Lorieux, Mathias Alvarez, Maria Fernanda Chavarriaga, Paul |
| author_sort | Nanez, Jhojan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice hoja blanca virus (RHBV), transmitted by the insect vector Tagosodes orizicolus, poses a significant threat to rice cultivation. Here, we use CRISPR/ Cas9 technology to produce specific mutations in the AGO4 gene of Oryza sativa, using the Fedearroz 2000 variety, with the aim of elucidating the participation of the gene in resistance to RHBV. We obtained 14 edited plants that presented with deletions of one, two, and three nucleotides in the sequence of exon 23 of the AGO4 gene. Phenotypic evaluations showed an increase in susceptibility to RHBV in the edited lines. We identified the presence of RHBV in the leaf tissue of infected plants by amplifying the nucleoprotein, NS3, and NS4 genes of the virus. Using RT-qPCR, we analyzed the expression patterns of the AGO4 gene, showing that in the edited lines, the expression profiles are similar to the susceptible control. Furthermore, modeling of the tertiary structure of the AGO4 protein and its mutant variant demonstrated changes in the PIWI domain and the presence of the DDH catalytic triad, confirming its role in mediating resistance to RHBV. Our study reveals the functional importance of the rice AGO4 gene in RHBV resistance. |
| format | Journal Article |
| id | CGSpace174109 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1741092025-12-08T10:29:22Z Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure Nanez, Jhojan Valdes, Sandra Cruz Gallego, Maribel Rebolledo, Maria Camila Lorieux, Mathias Alvarez, Maria Fernanda Chavarriaga, Paul rice oryza-rice (plant) crispr Rice hoja blanca virus (RHBV), transmitted by the insect vector Tagosodes orizicolus, poses a significant threat to rice cultivation. Here, we use CRISPR/ Cas9 technology to produce specific mutations in the AGO4 gene of Oryza sativa, using the Fedearroz 2000 variety, with the aim of elucidating the participation of the gene in resistance to RHBV. We obtained 14 edited plants that presented with deletions of one, two, and three nucleotides in the sequence of exon 23 of the AGO4 gene. Phenotypic evaluations showed an increase in susceptibility to RHBV in the edited lines. We identified the presence of RHBV in the leaf tissue of infected plants by amplifying the nucleoprotein, NS3, and NS4 genes of the virus. Using RT-qPCR, we analyzed the expression patterns of the AGO4 gene, showing that in the edited lines, the expression profiles are similar to the susceptible control. Furthermore, modeling of the tertiary structure of the AGO4 protein and its mutant variant demonstrated changes in the PIWI domain and the presence of the DDH catalytic triad, confirming its role in mediating resistance to RHBV. Our study reveals the functional importance of the rice AGO4 gene in RHBV resistance. 2025-03-28 2025-04-10T05:17:35Z 2025-04-10T05:17:35Z Journal Article https://hdl.handle.net/10568/174109 en Open Access application/pdf Frontiers Media Nanez, J.; Valdes, S.; Cruz Gallego, M.; Rebolledo, M.C.; Lorieux, M.; Alvarez, M.F.; Chavarriaga, P. (2025) Revealing the role of the AGO4 gene against rice hoja blanca virus: from transformation to protein structure. Frontiers in Plant Science 16: 1517321. ISSN: 1664-462X |
| spellingShingle | rice oryza-rice (plant) crispr Nanez, Jhojan Valdes, Sandra Cruz Gallego, Maribel Rebolledo, Maria Camila Lorieux, Mathias Alvarez, Maria Fernanda Chavarriaga, Paul Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure |
| title | Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure |
| title_full | Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure |
| title_fullStr | Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure |
| title_full_unstemmed | Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure |
| title_short | Revealing the role of the AGO4 gene against rice hoja blanca virus: From transformation to protein structure |
| title_sort | revealing the role of the ago4 gene against rice hoja blanca virus from transformation to protein structure |
| topic | rice oryza-rice (plant) crispr |
| url | https://hdl.handle.net/10568/174109 |
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