Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi
<i>Fasciola gigantica</i> is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of <i>F. gigantica</i> is important to understanding the parasite's transmission pa...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/174013 |
| _version_ | 1855518480901603328 |
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| author | Mogha, L. Kainga, H. Kamanga, N. Kapalamula, T.F. Wood, C. Thomas, Lian F. Mutua, Florence K. Sargison, N. Hayashida, K. Tsutsumi, T. Hayashi, N. Nonaka, N. Nakao, R. Chatanga, E. |
| author_browse | Chatanga, E. Hayashi, N. Hayashida, K. Kainga, H. Kamanga, N. Kapalamula, T.F. Mogha, L. Mutua, Florence K. Nakao, R. Nonaka, N. Sargison, N. Thomas, Lian F. Tsutsumi, T. Wood, C. |
| author_facet | Mogha, L. Kainga, H. Kamanga, N. Kapalamula, T.F. Wood, C. Thomas, Lian F. Mutua, Florence K. Sargison, N. Hayashida, K. Tsutsumi, T. Hayashi, N. Nonaka, N. Nakao, R. Chatanga, E. |
| author_sort | Mogha, L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | <i>Fasciola gigantica</i> is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of <i>F. gigantica</i> is important to understanding the parasite's transmission patterns/ and in monitoring the development of resistance to commonly used anthelmintic agents. This study aimed to analyze the genetic diversity and population structure of <i>Fasciola</i> species collected from cattle at slaughter slabs and abattoirs in selected districts of Malawi. A total of 27 adult liver flukes were collected from cattle at slaughter slabs and abattoirs in the northern region (n = 12), central region (n = 5), and southern region (n = 10). The mitochondrial cytochrome c oxidase I (COI) gene and nicotinamide adenine dinucleotide dehydrogenase 1 (ND1) gene were amplified and the amplicons were sequenced for all samples. The sequences obtained were used to investigate genetic diversity through median-joining networks and phylogenetic analysis. Tajima’s D test and Fu’s Fs statistics were used to determine the population structure. Based on the analyzed COI and ND1 sequences, all samples were identified as <i>F. gigantica</i>. Single nucleotide polymorphisms (SNPs) were identified at 18 and 17 positions for COI and ND1 genes, resulting in 10 and 5 haplotypes, respectively. The haplotype diversities were 0.867 and 0.479 for COI and ND1 gene sequences, respectively. The population genetic structure indices showed a population that has undergone a recent expansion. This study provides baseline epidemiological data on the genetic diversity and population structure of <i>F. gigantica</i> in Malawi; which is important for its control. |
| format | Journal Article |
| id | CGSpace174013 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1740132025-10-26T12:54:26Z Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi Mogha, L. Kainga, H. Kamanga, N. Kapalamula, T.F. Wood, C. Thomas, Lian F. Mutua, Florence K. Sargison, N. Hayashida, K. Tsutsumi, T. Hayashi, N. Nonaka, N. Nakao, R. Chatanga, E. animal health cattle epidemiology parasites <i>Fasciola gigantica</i> is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of <i>F. gigantica</i> is important to understanding the parasite's transmission patterns/ and in monitoring the development of resistance to commonly used anthelmintic agents. This study aimed to analyze the genetic diversity and population structure of <i>Fasciola</i> species collected from cattle at slaughter slabs and abattoirs in selected districts of Malawi. A total of 27 adult liver flukes were collected from cattle at slaughter slabs and abattoirs in the northern region (n = 12), central region (n = 5), and southern region (n = 10). The mitochondrial cytochrome c oxidase I (COI) gene and nicotinamide adenine dinucleotide dehydrogenase 1 (ND1) gene were amplified and the amplicons were sequenced for all samples. The sequences obtained were used to investigate genetic diversity through median-joining networks and phylogenetic analysis. Tajima’s D test and Fu’s Fs statistics were used to determine the population structure. Based on the analyzed COI and ND1 sequences, all samples were identified as <i>F. gigantica</i>. Single nucleotide polymorphisms (SNPs) were identified at 18 and 17 positions for COI and ND1 genes, resulting in 10 and 5 haplotypes, respectively. The haplotype diversities were 0.867 and 0.479 for COI and ND1 gene sequences, respectively. The population genetic structure indices showed a population that has undergone a recent expansion. This study provides baseline epidemiological data on the genetic diversity and population structure of <i>F. gigantica</i> in Malawi; which is important for its control. 2025-06 2025-04-04T12:25:55Z 2025-04-04T12:25:55Z Journal Article https://hdl.handle.net/10568/174013 en Limited Access Springer Mogha, L., Kainga, H., Kamanga, N., Kapalamula, T.F., Wood, C., Thomas, L.F., Mutua, F., Sargison, N., Hayashida, K., Tsutsumi, T., Hayashi, N., Nonaka, N., Nakao, R. and Chatanga, E. 2025. Genetic diversity and population structure of <i>Fasciola gigantica</i> isolated from cattle in Malawi. Veterinary Research Communications 49(3): 157. |
| spellingShingle | animal health cattle epidemiology parasites Mogha, L. Kainga, H. Kamanga, N. Kapalamula, T.F. Wood, C. Thomas, Lian F. Mutua, Florence K. Sargison, N. Hayashida, K. Tsutsumi, T. Hayashi, N. Nonaka, N. Nakao, R. Chatanga, E. Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi |
| title | Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi |
| title_full | Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi |
| title_fullStr | Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi |
| title_full_unstemmed | Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi |
| title_short | Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi |
| title_sort | genetic diversity and population structure of fasciola gigantica isolated from cattle in malawi |
| topic | animal health cattle epidemiology parasites |
| url | https://hdl.handle.net/10568/174013 |
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