Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda
<i>Leptospira</i> are bacteria that cause leptospirosis in both humans and animals. Human <i>Leptospira</i> infections in Uganda are suspected to arise from animal–human interactions. From a nationwide survey to determine Leptospira prevalence and circulating sequence types in Uganda, we tested 2030...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Cambridge University Press
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/173963 |
| _version_ | 1855522597862637568 |
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| author | Alinaitwe, Lordrick Wainaina, Martin Dürr, S. Kankya, C. Kivali, Velma Bugeza, James Aturinda, C.J. Lubega, A. Mayer-Scholl, A. Hoona, J.J. Bahn, P. Hammerl, J.A. Roesel, Kristina Cook, Elizabeth A.J. Richter, M.H. |
| author_browse | Alinaitwe, Lordrick Aturinda, C.J. Bahn, P. Bugeza, James Cook, Elizabeth A.J. Dürr, S. Hammerl, J.A. Hoona, J.J. Kankya, C. Kivali, Velma Lubega, A. Mayer-Scholl, A. Richter, M.H. Roesel, Kristina Wainaina, Martin |
| author_facet | Alinaitwe, Lordrick Wainaina, Martin Dürr, S. Kankya, C. Kivali, Velma Bugeza, James Aturinda, C.J. Lubega, A. Mayer-Scholl, A. Hoona, J.J. Bahn, P. Hammerl, J.A. Roesel, Kristina Cook, Elizabeth A.J. Richter, M.H. |
| author_sort | Alinaitwe, Lordrick |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | <i>Leptospira</i> are bacteria that cause leptospirosis in both humans and animals. Human <i>Leptospira</i> infections in Uganda are suspected to arise from animal–human interactions. From a nationwide survey to determine Leptospira prevalence and circulating sequence types in Uganda, we tested 2030 livestock kidney samples, and 117 small mammals (rodents and shrews) using real-time PCR targeting the lipL32 gene. Pathogenic <i>Leptospira</i> species were detected in 45 livestock samples but not in the small mammals. The prevalence was 6.12% in sheep, 4.25% in cattle, 2.08% in goats, and 0.46% in pigs. Sequence typing revealed that <i>Leptospira borgpetersenii</i>, <i>Leptospira kirschneri</i>, and <i>Leptospira interrogans</i> are widespread across Uganda, with 13 novel sequence types identified. These findings enhance the East African MLST database and support the hypothesis that domesticated animals may be a source of human leptospirosis in Uganda, highlighting the need for increased awareness among those in close contact with livestock. |
| format | Journal Article |
| id | CGSpace173963 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace1739632025-10-26T12:53:19Z Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda Alinaitwe, Lordrick Wainaina, Martin Dürr, S. Kankya, C. Kivali, Velma Bugeza, James Aturinda, C.J. Lubega, A. Mayer-Scholl, A. Hoona, J.J. Bahn, P. Hammerl, J.A. Roesel, Kristina Cook, Elizabeth A.J. Richter, M.H. cattle epidemiology goats leptospira livestock small ruminants swine zoonoses <i>Leptospira</i> are bacteria that cause leptospirosis in both humans and animals. Human <i>Leptospira</i> infections in Uganda are suspected to arise from animal–human interactions. From a nationwide survey to determine Leptospira prevalence and circulating sequence types in Uganda, we tested 2030 livestock kidney samples, and 117 small mammals (rodents and shrews) using real-time PCR targeting the lipL32 gene. Pathogenic <i>Leptospira</i> species were detected in 45 livestock samples but not in the small mammals. The prevalence was 6.12% in sheep, 4.25% in cattle, 2.08% in goats, and 0.46% in pigs. Sequence typing revealed that <i>Leptospira borgpetersenii</i>, <i>Leptospira kirschneri</i>, and <i>Leptospira interrogans</i> are widespread across Uganda, with 13 novel sequence types identified. These findings enhance the East African MLST database and support the hypothesis that domesticated animals may be a source of human leptospirosis in Uganda, highlighting the need for increased awareness among those in close contact with livestock. 2025 2025-04-02T05:23:03Z 2025-04-02T05:23:03Z Journal Article https://hdl.handle.net/10568/173963 en Open Access Cambridge University Press Alinaitwe, L., Wainaina, M., Dürr, S., Kankya, C., Kivali, V., Bugeza, J., Aturinda, C.J., Lubega, A., Mayer-Scholl, A., Hoona, J.J., Bahn, P., Hammerl, J.A., Roesel, K., Cook, E.A.J. and Richter, M.H. 2025. Molecular detection and typing of pathogenic <i>Leptospira</i> species from livestock and small mammals in Uganda. Epidemiology and Infection 153: e50. |
| spellingShingle | cattle epidemiology goats leptospira livestock small ruminants swine zoonoses Alinaitwe, Lordrick Wainaina, Martin Dürr, S. Kankya, C. Kivali, Velma Bugeza, James Aturinda, C.J. Lubega, A. Mayer-Scholl, A. Hoona, J.J. Bahn, P. Hammerl, J.A. Roesel, Kristina Cook, Elizabeth A.J. Richter, M.H. Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda |
| title | Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda |
| title_full | Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda |
| title_fullStr | Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda |
| title_full_unstemmed | Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda |
| title_short | Molecular detection and typing of pathogenic Leptospira species from livestock and small mammals in Uganda |
| title_sort | molecular detection and typing of pathogenic leptospira species from livestock and small mammals in uganda |
| topic | cattle epidemiology goats leptospira livestock small ruminants swine zoonoses |
| url | https://hdl.handle.net/10568/173963 |
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