Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes
Septoria tritici blotch (STB) is a predominant foliar disease of wheat, caused by the pathogen Zymoseptoria tritici. This disease can lead to substantial yield losses warranting control by using expensive fungicides. One effective method of STB control is the utilization of resistant wheat varieties...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Public Library of Science
2025
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/173945 |
| _version_ | 1855522287814443008 |
|---|---|
| author | Kassie, Molla Mekonnen Dejene, Tiegist Desta, Ermias Abate Tadesse, Wuletaw |
| author_browse | Dejene, Tiegist Desta, Ermias Abate Kassie, Molla Mekonnen Tadesse, Wuletaw |
| author_facet | Kassie, Molla Mekonnen Dejene, Tiegist Desta, Ermias Abate Tadesse, Wuletaw |
| author_sort | Kassie, Molla Mekonnen |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Septoria tritici blotch (STB) is a predominant foliar disease of wheat, caused by the pathogen Zymoseptoria tritici. This disease can lead to substantial yield losses warranting control by using expensive fungicides. One effective method of STB control is the utilization of resistant wheat varieties. In this particular study, a panel comprising of 186 bread wheat genotypes was assessed for their adult plant resistance (APR) to STB. Field trials were conducted across five environments in Ethiopia during the 2022 and 2023 growing seasons under natural infestation conditions. The association panel was genotyped using 20K single nucleotide polymorphism (SNP) markers. To determine the relationship between genetic markers and STB resistance, a mixed linear model (MLM) analysis was performed using the statgen GWAS R software package. Heritability estimates for STB resistance ranged from 0.39 to 0.95, underscoring the genetic variability and the potential for selection. The study identified 52 marker-trait associations (MTAs) for STB resistance at maturity (SDSM) and 62 MTAs at heading (SDSH). Chromosome 5A contains a high concentration of MTAs that confer resistance to STB, hosting multiple significant MTAs, including four consistently associated markers (‘Kukri_c10033_724’, ‘RAC875_rep_c116420_103’, ‘TG0019’, and ‘RAC875_c30566_230’). Additionally, chromosomes 1B, 2B, 5B, and 7A were found to harbor important MTAs, contributing to resistance across various environments. Notably, two QTLs, qtSTB23 (5A) and qtSTB38 (7B), exhibited stability across multiple environments, making them robust candidates for breeding programs. Furthermore, novel resistance loci on chromosome 2A were discovered, offering new opportunities for enhancing resistance. Therefore, these findings provide an opportunity for improving STB resistance through gene stacking using marker-assisted selection (MAS). |
| format | Journal Article |
| id | CGSpace173945 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1739452026-01-14T02:05:37Z Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes Kassie, Molla Mekonnen Dejene, Tiegist Desta, Ermias Abate Tadesse, Wuletaw bread wheat genome-wide septoria tritici blotch Septoria tritici blotch (STB) is a predominant foliar disease of wheat, caused by the pathogen Zymoseptoria tritici. This disease can lead to substantial yield losses warranting control by using expensive fungicides. One effective method of STB control is the utilization of resistant wheat varieties. In this particular study, a panel comprising of 186 bread wheat genotypes was assessed for their adult plant resistance (APR) to STB. Field trials were conducted across five environments in Ethiopia during the 2022 and 2023 growing seasons under natural infestation conditions. The association panel was genotyped using 20K single nucleotide polymorphism (SNP) markers. To determine the relationship between genetic markers and STB resistance, a mixed linear model (MLM) analysis was performed using the statgen GWAS R software package. Heritability estimates for STB resistance ranged from 0.39 to 0.95, underscoring the genetic variability and the potential for selection. The study identified 52 marker-trait associations (MTAs) for STB resistance at maturity (SDSM) and 62 MTAs at heading (SDSH). Chromosome 5A contains a high concentration of MTAs that confer resistance to STB, hosting multiple significant MTAs, including four consistently associated markers (‘Kukri_c10033_724’, ‘RAC875_rep_c116420_103’, ‘TG0019’, and ‘RAC875_c30566_230’). Additionally, chromosomes 1B, 2B, 5B, and 7A were found to harbor important MTAs, contributing to resistance across various environments. Notably, two QTLs, qtSTB23 (5A) and qtSTB38 (7B), exhibited stability across multiple environments, making them robust candidates for breeding programs. Furthermore, novel resistance loci on chromosome 2A were discovered, offering new opportunities for enhancing resistance. Therefore, these findings provide an opportunity for improving STB resistance through gene stacking using marker-assisted selection (MAS). 2025-03-31T19:32:05Z 2025-03-31T19:32:05Z Journal Article https://hdl.handle.net/10568/173945 en Open Access application/pdf Public Library of Science Molla Mekonnen Kassie, Tiegist Dejene, Ermias Abate Desta, Wuletaw Tadesse. (10/3/2025). Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes. PLoS ONE, 20 (3). |
| spellingShingle | bread wheat genome-wide septoria tritici blotch Kassie, Molla Mekonnen Dejene, Tiegist Desta, Ermias Abate Tadesse, Wuletaw Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes |
| title | Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes |
| title_full | Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes |
| title_fullStr | Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes |
| title_full_unstemmed | Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes |
| title_short | Genome-wide association analysis of Septoria tritici blotch for adult plant resistance in elite bread wheat (Triticum aestivum L) genotypes |
| title_sort | genome wide association analysis of septoria tritici blotch for adult plant resistance in elite bread wheat triticum aestivum l genotypes |
| topic | bread wheat genome-wide septoria tritici blotch |
| url | https://hdl.handle.net/10568/173945 |
| work_keys_str_mv | AT kassiemollamekonnen genomewideassociationanalysisofseptoriatriticiblotchforadultplantresistanceinelitebreadwheattriticumaestivumlgenotypes AT dejenetiegist genomewideassociationanalysisofseptoriatriticiblotchforadultplantresistanceinelitebreadwheattriticumaestivumlgenotypes AT destaermiasabate genomewideassociationanalysisofseptoriatriticiblotchforadultplantresistanceinelitebreadwheattriticumaestivumlgenotypes AT tadessewuletaw genomewideassociationanalysisofseptoriatriticiblotchforadultplantresistanceinelitebreadwheattriticumaestivumlgenotypes |