A synthetic genomics-based African swine fever virus engineering platform
African swine fever (ASF) is a deadly viral disease in domestic pigs that has a large global economic impact for the swine industry. It is present in Africa, Europe, Asia, and in the Caribbean island of Hispaniola. There are no effective treatments or broadly licensed vaccines to prevent disease. Ef...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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American Association for the Advancement of Science
2025
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| Online Access: | https://hdl.handle.net/10568/173910 |
| _version_ | 1855539522487451648 |
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| author | Fuchs, W. Assad-Garcia, N. Abkallo, Hussein M. Xue, Y. Oldfield, L.M. Fedorova, N. Hübner, A. Kabuuka, T. Pannhorst, K. Höper, D. Nene, Vishvanath Gonzalez-Juarbe, N. Steinaa, Lucilla Vashee, S. |
| author_browse | Abkallo, Hussein M. Assad-Garcia, N. Fedorova, N. Fuchs, W. Gonzalez-Juarbe, N. Höper, D. Hübner, A. Kabuuka, T. Nene, Vishvanath Oldfield, L.M. Pannhorst, K. Steinaa, Lucilla Vashee, S. Xue, Y. |
| author_facet | Fuchs, W. Assad-Garcia, N. Abkallo, Hussein M. Xue, Y. Oldfield, L.M. Fedorova, N. Hübner, A. Kabuuka, T. Pannhorst, K. Höper, D. Nene, Vishvanath Gonzalez-Juarbe, N. Steinaa, Lucilla Vashee, S. |
| author_sort | Fuchs, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African swine fever (ASF) is a deadly viral disease in domestic pigs that has a large global economic impact for the swine industry. It is present in Africa, Europe, Asia, and in the Caribbean island of Hispaniola. There are no effective treatments or broadly licensed vaccines to prevent disease. Efforts to counteract ASF have been hampered because of the lack of convenient tools to engineer its etiological agent, ASF virus (ASFV), largely due to its large noninfectious genome. Here, we report the use of synthetic genomics methodology to develop a reverse genetics system for ASFV using a CRISPR-Cas9–inhibited self-helper virus to reconstitute live recombinant ASFV from synthetic genomes to rapidly generate a variety of combinatorial mutants of ASFV. The method will substantially facilitate the development of therapeutics or subunit and live-attenuated vaccines for ASF. This synthetic genomics-based approach has wide-ranging impact because it can be applied to rapidly develop reverse genetics tools for emerging viruses with noninfectious genomes. |
| format | Journal Article |
| id | CGSpace173910 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | American Association for the Advancement of Science |
| publisherStr | American Association for the Advancement of Science |
| record_format | dspace |
| spelling | CGSpace1739102025-03-28T07:50:47Z A synthetic genomics-based African swine fever virus engineering platform Fuchs, W. Assad-Garcia, N. Abkallo, Hussein M. Xue, Y. Oldfield, L.M. Fedorova, N. Hübner, A. Kabuuka, T. Pannhorst, K. Höper, D. Nene, Vishvanath Gonzalez-Juarbe, N. Steinaa, Lucilla Vashee, S. african swine fever crispr disease control genomics swine African swine fever (ASF) is a deadly viral disease in domestic pigs that has a large global economic impact for the swine industry. It is present in Africa, Europe, Asia, and in the Caribbean island of Hispaniola. There are no effective treatments or broadly licensed vaccines to prevent disease. Efforts to counteract ASF have been hampered because of the lack of convenient tools to engineer its etiological agent, ASF virus (ASFV), largely due to its large noninfectious genome. Here, we report the use of synthetic genomics methodology to develop a reverse genetics system for ASFV using a CRISPR-Cas9–inhibited self-helper virus to reconstitute live recombinant ASFV from synthetic genomes to rapidly generate a variety of combinatorial mutants of ASFV. The method will substantially facilitate the development of therapeutics or subunit and live-attenuated vaccines for ASF. This synthetic genomics-based approach has wide-ranging impact because it can be applied to rapidly develop reverse genetics tools for emerging viruses with noninfectious genomes. 2025-03-28 2025-03-28T07:37:10Z 2025-03-28T07:37:10Z Journal Article https://hdl.handle.net/10568/173910 en Open Access American Association for the Advancement of Science Fuchs, W., Assad-Garcia, N., Abkallo, H.M., Xue, Y., Oldfield, L.M., Fedorova, N., Hübner, A., Kabuuka, T., Pannhorst, K., Höper, D., Nene, V., Gonzalez-Juarbe, N., Steinaa, L. and Vashee, S. 2025. A synthetic genomics-based African swine fever virus engineering platform. Science Advances 11(13): eadu7670. |
| spellingShingle | african swine fever crispr disease control genomics swine Fuchs, W. Assad-Garcia, N. Abkallo, Hussein M. Xue, Y. Oldfield, L.M. Fedorova, N. Hübner, A. Kabuuka, T. Pannhorst, K. Höper, D. Nene, Vishvanath Gonzalez-Juarbe, N. Steinaa, Lucilla Vashee, S. A synthetic genomics-based African swine fever virus engineering platform |
| title | A synthetic genomics-based African swine fever virus engineering platform |
| title_full | A synthetic genomics-based African swine fever virus engineering platform |
| title_fullStr | A synthetic genomics-based African swine fever virus engineering platform |
| title_full_unstemmed | A synthetic genomics-based African swine fever virus engineering platform |
| title_short | A synthetic genomics-based African swine fever virus engineering platform |
| title_sort | synthetic genomics based african swine fever virus engineering platform |
| topic | african swine fever crispr disease control genomics swine |
| url | https://hdl.handle.net/10568/173910 |
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