CRISPR-mediated genome editing of wheat for enhancing disease resistance

Wheat is cultivated across diverse global environments, and its productivity is significantly impacted by various biotic stresses, most importantly but not limited to rust diseases, Fusarium head blight, wheat blast, and powdery mildew. The genetic diversity of modern cultivars has been eroded by do...

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Main Authors: Waites, Joshua O., Achary, Mohan Murali, David, Easter S., Hearne, Sarah, Bandyopadhyay, Anindya
Format: Journal Article
Language:Inglés
Published: Frontiers Media 2025
Subjects:
Online Access:https://hdl.handle.net/10568/173898
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author Waites, Joshua O.
Achary, Mohan Murali
David, Easter S.
Hearne, Sarah
Bandyopadhyay, Anindya
author_browse Achary, Mohan Murali
Bandyopadhyay, Anindya
David, Easter S.
Hearne, Sarah
Waites, Joshua O.
author_facet Waites, Joshua O.
Achary, Mohan Murali
David, Easter S.
Hearne, Sarah
Bandyopadhyay, Anindya
author_sort Waites, Joshua O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Wheat is cultivated across diverse global environments, and its productivity is significantly impacted by various biotic stresses, most importantly but not limited to rust diseases, Fusarium head blight, wheat blast, and powdery mildew. The genetic diversity of modern cultivars has been eroded by domestication and selection, increasing their vulnerability to biotic stress due to uniformity. The rapid spread of new highly virulent and aggressive pathogen strains has exacerbated this situation. Three strategies can be used for enhancing disease resistance through genome editing: introducing resistance (R) gene-mediated resistance, engineering nucleotide-binding leucine-rich repeat receptors (NLRs), and manipulating susceptibility (S) genes to stop pathogens from exploiting these factors to support infection. Utilizing R gene-mediated resistance is the most common strategy for traditional breeding approaches, but the continuous evolution of pathogen effectors can eventually overcome this resistance. Moreover, modifying S genes can confer pleiotropic effects that hinder their use in agriculture. Enhancing disease resistance is paramount for sustainable wheat production and food security, and new tools and strategies are of great importance to the research community. The application of CRISPR-based genome editing provides promise to improve disease resistance, allowing access to a broader range of solutions beyond random mutagenesis or intraspecific variation, unlocking new ways to improve crops, and speeding up resistance breeding. Here, we first summarize the major disease resistance strategies in the context of important wheat diseases and their limitations. Next, we turn our attention to the powerful applications of genome editing technology in creating new wheat varieties against important wheat diseases.
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spelling CGSpace1738982025-12-08T10:29:22Z CRISPR-mediated genome editing of wheat for enhancing disease resistance Waites, Joshua O. Achary, Mohan Murali David, Easter S. Hearne, Sarah Bandyopadhyay, Anindya gene editing wheat crispr disease resistance Wheat is cultivated across diverse global environments, and its productivity is significantly impacted by various biotic stresses, most importantly but not limited to rust diseases, Fusarium head blight, wheat blast, and powdery mildew. The genetic diversity of modern cultivars has been eroded by domestication and selection, increasing their vulnerability to biotic stress due to uniformity. The rapid spread of new highly virulent and aggressive pathogen strains has exacerbated this situation. Three strategies can be used for enhancing disease resistance through genome editing: introducing resistance (R) gene-mediated resistance, engineering nucleotide-binding leucine-rich repeat receptors (NLRs), and manipulating susceptibility (S) genes to stop pathogens from exploiting these factors to support infection. Utilizing R gene-mediated resistance is the most common strategy for traditional breeding approaches, but the continuous evolution of pathogen effectors can eventually overcome this resistance. Moreover, modifying S genes can confer pleiotropic effects that hinder their use in agriculture. Enhancing disease resistance is paramount for sustainable wheat production and food security, and new tools and strategies are of great importance to the research community. The application of CRISPR-based genome editing provides promise to improve disease resistance, allowing access to a broader range of solutions beyond random mutagenesis or intraspecific variation, unlocking new ways to improve crops, and speeding up resistance breeding. Here, we first summarize the major disease resistance strategies in the context of important wheat diseases and their limitations. Next, we turn our attention to the powerful applications of genome editing technology in creating new wheat varieties against important wheat diseases. 2025-02-25 2025-03-27T13:29:08Z 2025-03-27T13:29:08Z Journal Article https://hdl.handle.net/10568/173898 en Open Access application/pdf Frontiers Media Waites, J., Achary, V. M. M., Syombua, E. D., Hearne, S. J., & Bandyopadhyay, A. (2025). CRISPR-mediated genome editing of wheat for enhancing disease resistance. Frontiers in Genome Editing, 7, 1542487. https://doi.org/10.3389/fgeed.2025.1542487
spellingShingle gene editing
wheat
crispr
disease resistance
Waites, Joshua O.
Achary, Mohan Murali
David, Easter S.
Hearne, Sarah
Bandyopadhyay, Anindya
CRISPR-mediated genome editing of wheat for enhancing disease resistance
title CRISPR-mediated genome editing of wheat for enhancing disease resistance
title_full CRISPR-mediated genome editing of wheat for enhancing disease resistance
title_fullStr CRISPR-mediated genome editing of wheat for enhancing disease resistance
title_full_unstemmed CRISPR-mediated genome editing of wheat for enhancing disease resistance
title_short CRISPR-mediated genome editing of wheat for enhancing disease resistance
title_sort crispr mediated genome editing of wheat for enhancing disease resistance
topic gene editing
wheat
crispr
disease resistance
url https://hdl.handle.net/10568/173898
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AT hearnesarah crisprmediatedgenomeeditingofwheatforenhancingdiseaseresistance
AT bandyopadhyayanindya crisprmediatedgenomeeditingofwheatforenhancingdiseaseresistance