A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network
Iron (Fe) homeostasis in plants is governed by a complex network of regulatory elements and transcription factors (TFs), as both Fe toxicity and deficiency negatively impact plant growth and physiology. The Fe homeostasis network is well characterized in Arabidopsis thaliana and remains poorly under...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/171486 |
| _version_ | 1855530913234944000 |
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| author | Carey-Fung, Oscar O'Brien, Martin Beasley, Jesse T. Johnson, Alexander A. T. |
| author_browse | Beasley, Jesse T. Carey-Fung, Oscar Johnson, Alexander A. T. O'Brien, Martin |
| author_facet | Carey-Fung, Oscar O'Brien, Martin Beasley, Jesse T. Johnson, Alexander A. T. |
| author_sort | Carey-Fung, Oscar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Iron (Fe) homeostasis in plants is governed by a complex network of regulatory elements and transcription factors (TFs), as both Fe toxicity and deficiency negatively impact plant growth and physiology. The Fe homeostasis network is well characterized in Arabidopsis thaliana and remains poorly understood in monocotyledon species such as rice (Oryza sativa L.). Recent investigation of the rice Fe homeostasis network revealed OsIRO3, a basic Helix–Loop–Helix (bHLH) TF as a putative negative regulator of genes involved in Fe uptake, transport, and storage. We employed CRISPR-Cas9 gene editing to target the OsIRO3 coding sequence and generate two independent T-DNA-free, loss-of-function iro3 mutants in rice cv. Nipponbare. The iro3 mutant plants had similar phenotype under nutrient-sufficient conditions and had stunted growth under Fe-deficient conditions, relative to a T-DNA free, wild-type control (WT). Under Fe deficiency, iro3 mutant shoots had reduced expression of Fe chelator biosynthetic genes (OsNAS1, OsNAS2, and OsNAAT1) and upregulated expression of an Fe transporter gene (OsYSL15), relative to WT shoots. We place our results in the context of the existing literature and generate a model describing the role of OsIRO3 in rice Fe homeostasis and reinforce the essential function of OsIRO3 in the rice Fe deficiency response. |
| format | Journal Article |
| id | CGSpace171486 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1714862025-12-08T10:29:22Z A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network Carey-Fung, Oscar O'Brien, Martin Beasley, Jesse T. Johnson, Alexander A. T. models helix homeostasis gene editing Iron (Fe) homeostasis in plants is governed by a complex network of regulatory elements and transcription factors (TFs), as both Fe toxicity and deficiency negatively impact plant growth and physiology. The Fe homeostasis network is well characterized in Arabidopsis thaliana and remains poorly understood in monocotyledon species such as rice (Oryza sativa L.). Recent investigation of the rice Fe homeostasis network revealed OsIRO3, a basic Helix–Loop–Helix (bHLH) TF as a putative negative regulator of genes involved in Fe uptake, transport, and storage. We employed CRISPR-Cas9 gene editing to target the OsIRO3 coding sequence and generate two independent T-DNA-free, loss-of-function iro3 mutants in rice cv. Nipponbare. The iro3 mutant plants had similar phenotype under nutrient-sufficient conditions and had stunted growth under Fe-deficient conditions, relative to a T-DNA free, wild-type control (WT). Under Fe deficiency, iro3 mutant shoots had reduced expression of Fe chelator biosynthetic genes (OsNAS1, OsNAS2, and OsNAAT1) and upregulated expression of an Fe transporter gene (OsYSL15), relative to WT shoots. We place our results in the context of the existing literature and generate a model describing the role of OsIRO3 in rice Fe homeostasis and reinforce the essential function of OsIRO3 in the rice Fe deficiency response. 2022 2025-01-29T12:58:14Z 2025-01-29T12:58:14Z Journal Article https://hdl.handle.net/10568/171486 en Open Access MDPI Carey-Fung, Oscar; O'Brien, Martin; Beasley, Jesse T.; and Johnson, Alexander A. T. 2022. A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network. International Journal of Molecular Sciences 23(3): 1635. https://doi.org/10.3390/ijms23031635 |
| spellingShingle | models helix homeostasis gene editing Carey-Fung, Oscar O'Brien, Martin Beasley, Jesse T. Johnson, Alexander A. T. A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network |
| title | A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network |
| title_full | A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network |
| title_fullStr | A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network |
| title_full_unstemmed | A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network |
| title_short | A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network |
| title_sort | model to incorporate the bhlh transcription factor osiro3 within the rice iron homeostasis regulatory network |
| topic | models helix homeostasis gene editing |
| url | https://hdl.handle.net/10568/171486 |
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