The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial
Background: Zinc biofortification of rice could sustainably improve zinc status in countries where zinc deficiency is common and rice is a staple, but its efficacy has not been tested. Fatty acid desaturases (FADS) are putative new zinc status biomarkers. Objective: Our objective was to test the eff...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/171480 |
| _version_ | 1855524710714966016 |
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| author | Jongstra, Roelinda Mokbul Hossain, Md Galetti, Valeria Hall, Andrew G. Holt, Roberta R. |
| author_browse | Galetti, Valeria Hall, Andrew G. Holt, Roberta R. Jongstra, Roelinda Mokbul Hossain, Md |
| author_facet | Jongstra, Roelinda Mokbul Hossain, Md Galetti, Valeria Hall, Andrew G. Holt, Roberta R. |
| author_sort | Jongstra, Roelinda |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Zinc biofortification of rice could sustainably improve zinc status in countries where zinc deficiency is common and rice is a staple, but its efficacy has not been tested. Fatty acid desaturases (FADS) are putative new zinc status biomarkers. Objective: Our objective was to test the efficacy of zinc-biofortified rice in preschool-aged children with zinc-deficiency. Our hypothesis was that consumption of zinc-biofortified rice would increase plasma zinc concentration (PZC). Design: We conducted a 9-month, double-masked, intervention trial in 12–36 month-old rural Bangladeshi children, most of whem were who were zinc-deficient (PZC < 70 µg/dL) and stunted (n = 520). The children were randomized to receive either control rice (CR) or zinc-biofortified rice (BFR) provided in cooked portions to their households daily, with compliance monitoring. The primary outcome was PZC. Secondary outcomes were zinc deficiency, linear growth, infection-related morbidity, FADS activity indices, intestinal fatty acid binding protein (I-FABP) and fecal calprotectin. We applied sparse serial sampling for midpoint measures and analyzed data by intention-to-treat using mixed-effects models. The trial was registered under NCT03079583. Results: At baseline, median (IQR) PZC was 60.4 (56.3–64.3) µg/dL, 78.1% of children were zinc deficient and 59.7% were stunted. Mean ± SD daily zinc intakes from the CR and BFR during the trial were 1.20 ± 0.34 and 2.22 ± 0.47 mg/day, respectively (P < 0.001). There were no significant time-by-treatment effects on PZC, zinc deficiency prevalence, FADS activity, I-FABP or fecal calprotectin (all P > 0.05). There was a time-treatment interaction for height-for-age z scores (P < 0.001) favoring the BFR group. The morbidity longitudinal prevalence ratio (LPR) (95% CI) was 1.08 (1.05,1.12) comparing the BFR and CR groups, due to more upper respiratory tract illness in the BFR group. Conclusions: Consumption of zinc-biofortified rice for 9 months providing ∼1 mg of additional zinc daily to Bangladeshi children did not significantly affect PZC, prevalence of zinc deficiency or FADS activity. Clinical Trial Registry: https://clinicaltrials.gov/ct2/show/NCT03079583 |
| format | Journal Article |
| id | CGSpace171480 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1714802025-10-28T10:12:08Z The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial Jongstra, Roelinda Mokbul Hossain, Md Galetti, Valeria Hall, Andrew G. Holt, Roberta R. zinc biofortification schoolchildren children rice trace elements Background: Zinc biofortification of rice could sustainably improve zinc status in countries where zinc deficiency is common and rice is a staple, but its efficacy has not been tested. Fatty acid desaturases (FADS) are putative new zinc status biomarkers. Objective: Our objective was to test the efficacy of zinc-biofortified rice in preschool-aged children with zinc-deficiency. Our hypothesis was that consumption of zinc-biofortified rice would increase plasma zinc concentration (PZC). Design: We conducted a 9-month, double-masked, intervention trial in 12–36 month-old rural Bangladeshi children, most of whem were who were zinc-deficient (PZC < 70 µg/dL) and stunted (n = 520). The children were randomized to receive either control rice (CR) or zinc-biofortified rice (BFR) provided in cooked portions to their households daily, with compliance monitoring. The primary outcome was PZC. Secondary outcomes were zinc deficiency, linear growth, infection-related morbidity, FADS activity indices, intestinal fatty acid binding protein (I-FABP) and fecal calprotectin. We applied sparse serial sampling for midpoint measures and analyzed data by intention-to-treat using mixed-effects models. The trial was registered under NCT03079583. Results: At baseline, median (IQR) PZC was 60.4 (56.3–64.3) µg/dL, 78.1% of children were zinc deficient and 59.7% were stunted. Mean ± SD daily zinc intakes from the CR and BFR during the trial were 1.20 ± 0.34 and 2.22 ± 0.47 mg/day, respectively (P < 0.001). There were no significant time-by-treatment effects on PZC, zinc deficiency prevalence, FADS activity, I-FABP or fecal calprotectin (all P > 0.05). There was a time-treatment interaction for height-for-age z scores (P < 0.001) favoring the BFR group. The morbidity longitudinal prevalence ratio (LPR) (95% CI) was 1.08 (1.05,1.12) comparing the BFR and CR groups, due to more upper respiratory tract illness in the BFR group. Conclusions: Consumption of zinc-biofortified rice for 9 months providing ∼1 mg of additional zinc daily to Bangladeshi children did not significantly affect PZC, prevalence of zinc deficiency or FADS activity. Clinical Trial Registry: https://clinicaltrials.gov/ct2/show/NCT03079583 2022-03 2025-01-29T12:58:14Z 2025-01-29T12:58:14Z Journal Article https://hdl.handle.net/10568/171480 en Open Access Elsevier Jongstra, Roelinda; Mokbul Hossain, Md; Galetti, Valeria; Hall, Andrew G.; Holt, Roberta R.; et al. 2022. The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial. American Journal of Clinical Nutrition 115(3): 724-737. https://doi.org/10.1093/ajcn/nqab379 |
| spellingShingle | zinc biofortification schoolchildren children rice trace elements Jongstra, Roelinda Mokbul Hossain, Md Galetti, Valeria Hall, Andrew G. Holt, Roberta R. The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial |
| title | The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial |
| title_full | The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial |
| title_fullStr | The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial |
| title_full_unstemmed | The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial |
| title_short | The effect of zinc-biofortified rice on zinc status of Bangladeshi pre-school children: A randomized, double-masked, household-based controlled trial |
| title_sort | effect of zinc biofortified rice on zinc status of bangladeshi pre school children a randomized double masked household based controlled trial |
| topic | zinc biofortification schoolchildren children rice trace elements |
| url | https://hdl.handle.net/10568/171480 |
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