Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils
Background: Vitamin A (VA) deficiency is a public health problem in some countries. Fortification, supplementation, and increased provitamin A consumption through biofortification are efficacious, but monitoring is needed due to risk of excessive VA intake when interventions overlap. Objectives: Two...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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American Society for Nutrition
2020
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| Online Access: | https://hdl.handle.net/10568/171394 |
| _version_ | 1855531426631385088 |
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| author | Sowa, Margaret Mourao, Luciana Sheftel, Jesse Kaeppler, Mikayla Simons, Gabrielle |
| author_browse | Kaeppler, Mikayla Mourao, Luciana Sheftel, Jesse Simons, Gabrielle Sowa, Margaret |
| author_facet | Sowa, Margaret Mourao, Luciana Sheftel, Jesse Kaeppler, Mikayla Simons, Gabrielle |
| author_sort | Sowa, Margaret |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Vitamin A (VA) deficiency is a public health problem in some countries. Fortification, supplementation, and increased provitamin A consumption through biofortification are efficacious, but monitoring is needed due to risk of excessive VA intake when interventions overlap. Objectives: Two studies in 28–36-d-old male Mongolian gerbils simulated exposure to multiple VA interventions to determine the effects of provitamin A carotenoid consumption from biofortified maize and carrots and preformed VA fortificant on status. Methods: Study 1 was a 2 × 2 × 2 factorial design (n = 85) with high-β-carotene maize, orange carrots, and VA fortification at 50% estimated gerbil needs, compared with white maize and white carrot controls. Study 2 was a 2 × 3 factorial design (n = 66) evaluating orange carrot and VA consumption through fortification at 100% and 200% estimated needs. Both studies utilized 2-wk VA depletion, baseline evaluation, 9-wk treatments, and liver VA stores by HPLC. Intestinal scavenger receptor class B member 1 (Scarb1), β-carotene 15,15′-dioxygenase (Bco1), β-carotene 9′,10′-oxygenase (Bco2), intestine-specific homeobox (Isx), and cytochrome P450 26A1 isoform α1 (Cyp26a1) expression was analyzed by qRT-PCR in study 2. Results: In study 1, liver VA concentrations were significantly higher in orange carrot (0.69 ± 0.12 μmol/g) and orange maize groups (0.52 ± 0.21 μmol/g) compared with baseline (0.23 ± 0.069 μmol/g) and controls. Liver VA concentrations from VA fortificant alone (0.11 ± 0.053 μmol/g) did not differ from negative control. In study 2, orange carrot significantly enhanced liver VA concentrations (0.85 ± 0.24 μmol/g) relative to baseline (0.43 ± 0.14 μmol/g), but VA fortificant alone (0.42 ± 0.21 μmol/g) did not. Intestinal Scarb1 and Bco1 were negatively correlated with increasing liver VA concentrations (P < 0.01, r2 = 0.25–0.27). Serum retinol concentrations did not differ. Conclusions: Biofortified carrots and maize without fortification prevented VA deficiency in gerbils. During adequate provitamin A dietary intake, preformed VA intake resulted in excessive liver stores in gerbils, despite downregulation of carotenoid absorption and cleavage gene expression. |
| format | Journal Article |
| id | CGSpace171394 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | American Society for Nutrition |
| publisherStr | American Society for Nutrition |
| record_format | dspace |
| spelling | CGSpace1713942025-01-29T12:58:07Z Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils Sowa, Margaret Mourao, Luciana Sheftel, Jesse Kaeppler, Mikayla Simons, Gabrielle biofortification retinol provitamins carotenoids maize carrots Background: Vitamin A (VA) deficiency is a public health problem in some countries. Fortification, supplementation, and increased provitamin A consumption through biofortification are efficacious, but monitoring is needed due to risk of excessive VA intake when interventions overlap. Objectives: Two studies in 28–36-d-old male Mongolian gerbils simulated exposure to multiple VA interventions to determine the effects of provitamin A carotenoid consumption from biofortified maize and carrots and preformed VA fortificant on status. Methods: Study 1 was a 2 × 2 × 2 factorial design (n = 85) with high-β-carotene maize, orange carrots, and VA fortification at 50% estimated gerbil needs, compared with white maize and white carrot controls. Study 2 was a 2 × 3 factorial design (n = 66) evaluating orange carrot and VA consumption through fortification at 100% and 200% estimated needs. Both studies utilized 2-wk VA depletion, baseline evaluation, 9-wk treatments, and liver VA stores by HPLC. Intestinal scavenger receptor class B member 1 (Scarb1), β-carotene 15,15′-dioxygenase (Bco1), β-carotene 9′,10′-oxygenase (Bco2), intestine-specific homeobox (Isx), and cytochrome P450 26A1 isoform α1 (Cyp26a1) expression was analyzed by qRT-PCR in study 2. Results: In study 1, liver VA concentrations were significantly higher in orange carrot (0.69 ± 0.12 μmol/g) and orange maize groups (0.52 ± 0.21 μmol/g) compared with baseline (0.23 ± 0.069 μmol/g) and controls. Liver VA concentrations from VA fortificant alone (0.11 ± 0.053 μmol/g) did not differ from negative control. In study 2, orange carrot significantly enhanced liver VA concentrations (0.85 ± 0.24 μmol/g) relative to baseline (0.43 ± 0.14 μmol/g), but VA fortificant alone (0.42 ± 0.21 μmol/g) did not. Intestinal Scarb1 and Bco1 were negatively correlated with increasing liver VA concentrations (P < 0.01, r2 = 0.25–0.27). Serum retinol concentrations did not differ. Conclusions: Biofortified carrots and maize without fortification prevented VA deficiency in gerbils. During adequate provitamin A dietary intake, preformed VA intake resulted in excessive liver stores in gerbils, despite downregulation of carotenoid absorption and cleavage gene expression. 2020-11 2025-01-29T12:58:07Z 2025-01-29T12:58:07Z Journal Article https://hdl.handle.net/10568/171394 en Open Access American Society for Nutrition Sowa, Margaret; Mourao, Luciana; Sheftel, Jesse; Kaeppler, Mikayla; Simons, Gabrielle; et al. 2020. Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils. Journal of Nutrition 150(11): 2912–2923. https://doi.org/10.1093/jn/nxaa142 |
| spellingShingle | biofortification retinol provitamins carotenoids maize carrots Sowa, Margaret Mourao, Luciana Sheftel, Jesse Kaeppler, Mikayla Simons, Gabrielle Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils |
| title | Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils |
| title_full | Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils |
| title_fullStr | Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils |
| title_full_unstemmed | Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils |
| title_short | Overlapping vitamin A interventions with provitamin A carotenoids and preformed vitamin A cause excessive liver retinol stores in male Mongolian gerbils |
| title_sort | overlapping vitamin a interventions with provitamin a carotenoids and preformed vitamin a cause excessive liver retinol stores in male mongolian gerbils |
| topic | biofortification retinol provitamins carotenoids maize carrots |
| url | https://hdl.handle.net/10568/171394 |
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