Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet
Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world. Biofortification through molecular breeding is the way forward to achieving a rapid trait-base...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/171302 |
| _version_ | 1855523279706521600 |
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| author | Pujar, Mahesh Gangaprasad, S. Govindaraj, Mahalingam Gangurde, Sunil S. Kanatti, A. Kudapa, Himabindu |
| author_browse | Gangaprasad, S. Gangurde, Sunil S. Govindaraj, Mahalingam Kanatti, A. Kudapa, Himabindu Pujar, Mahesh |
| author_facet | Pujar, Mahesh Gangaprasad, S. Govindaraj, Mahalingam Gangurde, Sunil S. Kanatti, A. Kudapa, Himabindu |
| author_sort | Pujar, Mahesh |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world. Biofortification through molecular breeding is the way forward to achieving a rapid trait-based breeding strategy. This genome-wide association study (GWAS) was conducted to identify significant marker-trait associations (MTAs) for Fe, Zn, and protein content (PC) for enhanced biofortification breeding. A diverse panel of 281 advanced inbred lines was evaluated for Fe, Zn, and PC over two seasons. Phenotypic evaluation revealed high variability (Fe: 32–120 mg kg−1, Zn: 19–87 mg kg−1, PC: 8–16%), heritability (hbs2 ≥ 90%) and significantly positive correlation among Fe, Zn and PC (P = 0.01), implying concurrent improvement. Based on the Diversity Arrays Technology (DArT) seq assay, 58,719 highly informative SNPs were filtered for association mapping. Population structure analysis showed six major genetic groups (K = 6). A total of 78 MTAs were identified, of which 18 were associated with Fe, 43 with Zn, and 17 with PC. Four SNPs viz., Pgl04_64673688, Pgl05_135500493, Pgl05_144482656, and Pgl07_101483782 located on chromosomes Pgl04 (1), Pgl05 (2) and Pgl07 (1), respectively were co-segregated for Fe and Zn. Promising genes, ‘Late embryogenesis abundant protein’, ‘Myb domain’, ‘pentatricopeptide repeat’, and ‘iron ion binding’ coded by 8 SNPs were identified. The SNPs/genes identified in the present study presents prospects for genomics assisted biofortification breeding in pearl millet. |
| format | Journal Article |
| id | CGSpace171302 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1713022025-01-29T12:57:59Z Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet Pujar, Mahesh Gangaprasad, S. Govindaraj, Mahalingam Gangurde, Sunil S. Kanatti, A. Kudapa, Himabindu iron zinc pearl millet biofortification crops trace elements nutrition grain proteins Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world. Biofortification through molecular breeding is the way forward to achieving a rapid trait-based breeding strategy. This genome-wide association study (GWAS) was conducted to identify significant marker-trait associations (MTAs) for Fe, Zn, and protein content (PC) for enhanced biofortification breeding. A diverse panel of 281 advanced inbred lines was evaluated for Fe, Zn, and PC over two seasons. Phenotypic evaluation revealed high variability (Fe: 32–120 mg kg−1, Zn: 19–87 mg kg−1, PC: 8–16%), heritability (hbs2 ≥ 90%) and significantly positive correlation among Fe, Zn and PC (P = 0.01), implying concurrent improvement. Based on the Diversity Arrays Technology (DArT) seq assay, 58,719 highly informative SNPs were filtered for association mapping. Population structure analysis showed six major genetic groups (K = 6). A total of 78 MTAs were identified, of which 18 were associated with Fe, 43 with Zn, and 17 with PC. Four SNPs viz., Pgl04_64673688, Pgl05_135500493, Pgl05_144482656, and Pgl07_101483782 located on chromosomes Pgl04 (1), Pgl05 (2) and Pgl07 (1), respectively were co-segregated for Fe and Zn. Promising genes, ‘Late embryogenesis abundant protein’, ‘Myb domain’, ‘pentatricopeptide repeat’, and ‘iron ion binding’ coded by 8 SNPs were identified. The SNPs/genes identified in the present study presents prospects for genomics assisted biofortification breeding in pearl millet. 2020 2025-01-29T12:57:59Z 2025-01-29T12:57:59Z Journal Article https://hdl.handle.net/10568/171302 en Open Access Springer Pujar, Mahesh; Gangaprasad, S.; Govindaraj, Mahalingam; Gangurde, Sunil S.; Kanatti, A.; and Kudapa, Himabindu. 2020. Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet. Scientific Reports 10: 19473. https://doi.org/10.1038/s41598-020-76230-y |
| spellingShingle | iron zinc pearl millet biofortification crops trace elements nutrition grain proteins Pujar, Mahesh Gangaprasad, S. Govindaraj, Mahalingam Gangurde, Sunil S. Kanatti, A. Kudapa, Himabindu Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet |
| title | Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet |
| title_full | Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet |
| title_fullStr | Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet |
| title_full_unstemmed | Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet |
| title_short | Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet |
| title_sort | genome wide association study uncovers genomic regions associated with grain iron zinc and protein content in pearl millet |
| topic | iron zinc pearl millet biofortification crops trace elements nutrition grain proteins |
| url | https://hdl.handle.net/10568/171302 |
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