Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells
Nicotianamine (NA) is a low-molecular weight metal chelator in plants with high affinity for ferrous iron (Fe2+) and other divalent metal cations. In graminaceous plant species, NA serves as the biosynthetic precursor to 2′ deoxymugineic acid (DMA), a root-secreted mugineic acid family phytosideroph...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/171189 |
| _version_ | 1855538841353453568 |
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| author | Beasley, Jesse T. Hart, Jonathan J. Tako, Elad Glahn, Raymond P. Johnson, Alexander A. T. |
| author_browse | Beasley, Jesse T. Glahn, Raymond P. Hart, Jonathan J. Johnson, Alexander A. T. Tako, Elad |
| author_facet | Beasley, Jesse T. Hart, Jonathan J. Tako, Elad Glahn, Raymond P. Johnson, Alexander A. T. |
| author_sort | Beasley, Jesse T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nicotianamine (NA) is a low-molecular weight metal chelator in plants with high affinity for ferrous iron (Fe2+) and other divalent metal cations. In graminaceous plant species, NA serves as the biosynthetic precursor to 2′ deoxymugineic acid (DMA), a root-secreted mugineic acid family phytosiderophore that chelates ferric iron (Fe3+) in the rhizosphere for subsequent uptake by the plant. Previous studies have flagged NA and/or DMA as enhancers of Fe bioavailability in cereal grain although the extent of this promotion has not been quantified. In this study, we utilized the Caco-2 cell system to compare NA and DMA to two known enhancers of Fe bioavailability—epicatechin (Epi) and ascorbic acid (AsA)—and found that both NA and DMA are stronger enhancers of Fe bioavailability than Epi, and NA is a stronger enhancer of Fe bioavailability than AsA. Furthermore, NA reversed Fe uptake inhibition by Myricetin (Myr) more than Epi, highlighting NA as an important target for biofortification strategies aimed at improving Fe bioavailability in staple plant foods. |
| format | Journal Article |
| id | CGSpace171189 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1711892025-01-29T12:57:50Z Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells Beasley, Jesse T. Hart, Jonathan J. Tako, Elad Glahn, Raymond P. Johnson, Alexander A. T. iron biofortification anaemia ferritin ascorbic acid Nicotianamine (NA) is a low-molecular weight metal chelator in plants with high affinity for ferrous iron (Fe2+) and other divalent metal cations. In graminaceous plant species, NA serves as the biosynthetic precursor to 2′ deoxymugineic acid (DMA), a root-secreted mugineic acid family phytosiderophore that chelates ferric iron (Fe3+) in the rhizosphere for subsequent uptake by the plant. Previous studies have flagged NA and/or DMA as enhancers of Fe bioavailability in cereal grain although the extent of this promotion has not been quantified. In this study, we utilized the Caco-2 cell system to compare NA and DMA to two known enhancers of Fe bioavailability—epicatechin (Epi) and ascorbic acid (AsA)—and found that both NA and DMA are stronger enhancers of Fe bioavailability than Epi, and NA is a stronger enhancer of Fe bioavailability than AsA. Furthermore, NA reversed Fe uptake inhibition by Myricetin (Myr) more than Epi, highlighting NA as an important target for biofortification strategies aimed at improving Fe bioavailability in staple plant foods. 2019 2025-01-29T12:57:50Z 2025-01-29T12:57:50Z Journal Article https://hdl.handle.net/10568/171189 en Open Access MDPI Beasley, Jesse T.; Hart, Jonathan J.; Tako, Elad; Glahn, Raymond P.; and Johnson, Alexander A.T. 2019. Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells. Nutrients 11(7): 1502. https://doi.org/10.3390/nu11071502 |
| spellingShingle | iron biofortification anaemia ferritin ascorbic acid Beasley, Jesse T. Hart, Jonathan J. Tako, Elad Glahn, Raymond P. Johnson, Alexander A. T. Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells |
| title | Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells |
| title_full | Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells |
| title_fullStr | Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells |
| title_full_unstemmed | Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells |
| title_short | Investigation of nicotianamine and 2′ deoxymugineic acid as enhancers of iron bioavailability in caco-2 cells |
| title_sort | investigation of nicotianamine and 2 deoxymugineic acid as enhancers of iron bioavailability in caco 2 cells |
| topic | iron biofortification anaemia ferritin ascorbic acid |
| url | https://hdl.handle.net/10568/171189 |
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