Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis
Low productivity of pilosae type lentils grown in South Asia is attributed to narrow genetic base of the released cultivars which results in susceptibility to biotic and abiotic stresses. For enhancement of productivity and production, broadening of genetic base is essentially required. The genetic...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Public Library of Science
2015
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| Acceso en línea: | https://hdl.handle.net/10568/170951 |
| _version_ | 1855520306101223424 |
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| author | Dikshit, Harsh Kumar Singh, Akanksha Singh, Dharmendra Aski, Muraleedhar Prakash, Prapti Jain, Neelu Meena, Suresh Kumar, Shiv Sarker, Ashutosh |
| author_browse | Aski, Muraleedhar Dikshit, Harsh Kumar Jain, Neelu Kumar, Shiv Meena, Suresh Prakash, Prapti Sarker, Ashutosh Singh, Akanksha Singh, Dharmendra |
| author_facet | Dikshit, Harsh Kumar Singh, Akanksha Singh, Dharmendra Aski, Muraleedhar Prakash, Prapti Jain, Neelu Meena, Suresh Kumar, Shiv Sarker, Ashutosh |
| author_sort | Dikshit, Harsh Kumar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Low productivity of pilosae type lentils grown in South Asia is attributed to narrow genetic base of the released cultivars which results in susceptibility to biotic and abiotic stresses. For enhancement of productivity and production, broadening of genetic base is essentially required. The genetic base of released cultivars can be broadened by using diverse types including bold seeded and early maturing lentils from Mediterranean region and related wild species. Genetic diversity in eighty six accessions of three species of genus Lens was assessed based on twelve genomic and thirty one EST-SSR markers. The evaluated set of genotypes included diverse lentil varieties and advanced breeding lines from Indian programme, two early maturing ICARDA lines and five related wild subspecies/species endemic to the Mediterranean region. Genomic SSRs exhibited higher polymorphism in comparison to EST SSRs. GLLC 598 produced 5 alleles with highest gene diversity value of 0.80. Among the studied subspecies/species 43 SSRs detected maximum number of alleles in L. orientalis. Based on Nei's genetic distance cultivated lentil L. culinaris subsp. culinaris was found to be close to its wild progenitor L. culinaris subsp. orientalis. The Prichard's structure of 86 genotypes distinguished different subspecies/species. Higher variability was recorded among individuals within population than among populations. |
| format | Journal Article |
| id | CGSpace170951 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1709512025-09-25T13:01:37Z Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis Dikshit, Harsh Kumar Singh, Akanksha Singh, Dharmendra Aski, Muraleedhar Prakash, Prapti Jain, Neelu Meena, Suresh Kumar, Shiv Sarker, Ashutosh biofortification lentils Low productivity of pilosae type lentils grown in South Asia is attributed to narrow genetic base of the released cultivars which results in susceptibility to biotic and abiotic stresses. For enhancement of productivity and production, broadening of genetic base is essentially required. The genetic base of released cultivars can be broadened by using diverse types including bold seeded and early maturing lentils from Mediterranean region and related wild species. Genetic diversity in eighty six accessions of three species of genus Lens was assessed based on twelve genomic and thirty one EST-SSR markers. The evaluated set of genotypes included diverse lentil varieties and advanced breeding lines from Indian programme, two early maturing ICARDA lines and five related wild subspecies/species endemic to the Mediterranean region. Genomic SSRs exhibited higher polymorphism in comparison to EST SSRs. GLLC 598 produced 5 alleles with highest gene diversity value of 0.80. Among the studied subspecies/species 43 SSRs detected maximum number of alleles in L. orientalis. Based on Nei's genetic distance cultivated lentil L. culinaris subsp. culinaris was found to be close to its wild progenitor L. culinaris subsp. orientalis. The Prichard's structure of 86 genotypes distinguished different subspecies/species. Higher variability was recorded among individuals within population than among populations. 2015 2025-01-29T12:57:31Z 2025-01-29T12:57:31Z Journal Article https://hdl.handle.net/10568/170951 en Open Access Public Library of Science Dikshit, Harsh Kumar; Singh, Akanksha; Singh, Dharmendra; Aski, Muraleedhar Sidaram; Prakash, Prapti; Jain, Neelu; Meena, Suresh; Kumar, Shiv; and Sarker, Ashutosh. 2015. Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis. PLoS ONE 10(9): e0138101. https://doi.org/10.1371/journal.pone.0138101 |
| spellingShingle | biofortification lentils Dikshit, Harsh Kumar Singh, Akanksha Singh, Dharmendra Aski, Muraleedhar Prakash, Prapti Jain, Neelu Meena, Suresh Kumar, Shiv Sarker, Ashutosh Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis |
| title | Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis |
| title_full | Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis |
| title_fullStr | Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis |
| title_full_unstemmed | Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis |
| title_short | Genetic diversity in lens species revealed by EST and genomic simple sequence repeat analysis |
| title_sort | genetic diversity in lens species revealed by est and genomic simple sequence repeat analysis |
| topic | biofortification lentils |
| url | https://hdl.handle.net/10568/170951 |
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