Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave
The fungus Pyricularia oryzae Cav., is the cause of the most important disease in rice cultivation, blast, due to its widespread presence in the world. Consequently, the development of rice plants tolerant to this disease is a key factor to maintain crop yields. By incorporating techniques such as...
| Main Authors: | , , , , |
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| Format: | Poster |
| Language: | Español |
| Published: |
2024
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| Online Access: | https://hdl.handle.net/10568/170301 |
| _version_ | 1855513707555061760 |
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| author | Pineda, Marcela Agrono, Tomas Quintero, Constanza Morales, Mabel Alvarez, Maria Fernanda |
| author_browse | Agrono, Tomas Alvarez, Maria Fernanda Morales, Mabel Pineda, Marcela Quintero, Constanza |
| author_facet | Pineda, Marcela Agrono, Tomas Quintero, Constanza Morales, Mabel Alvarez, Maria Fernanda |
| author_sort | Pineda, Marcela |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The fungus Pyricularia oryzae Cav., is the cause of the most important disease in rice cultivation, blast, due to its widespread presence in the world. Consequently, the development of rice plants tolerant to this disease is a key factor to maintain crop yields. By incorporating techniques such as marker-assisted selection (MAS) to screen genes of interest and the production of Double Haploid plants to generate homogeneous populations, we accelerate the development of resistant materials with several blast resistance genes. This study aims to obtain plants carrying five resistance genes to Pyricularia using molecular markers and tissue culture. Three parents were involved which have been identified as carriers of Pyricularia tolerance genes: CIFED 11 A (Pish, Pi2, and Piks-Zh), IRBLt-K59_LTH (Pi-t) and FL13032 (Pish and Pii). Two simple crosses were carried out (CIFED 11 A x IRBLt-K59 and CIFED 11 A x IRBLT-K59) and the F1 plants carrying the genes of each parent involved were selected, subsequently, A double cross was made between the two F1 to obtain a population of 1424 seeds that were planted to confirm the presence of the genes and with the selected plants the anther culture was carried out. In the tissue culture process, calluses were obtained, and plants were regenerated as a result of these. According to the phenotypic evaluation, double haploid plants were identified and evaluated to confirm the presence of the five resistance genes. Seven plants were confirmed as carriers of the genes of interest that present genotypic and phenotypic stability, which can be included in breeding program schemes for the generation of varieties tolerant to Pyricularia. |
| format | Poster |
| id | CGSpace170301 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| record_format | dspace |
| spelling | CGSpace1703012025-12-08T10:06:44Z Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave Pineda, Marcela Agrono, Tomas Quintero, Constanza Morales, Mabel Alvarez, Maria Fernanda rice arroz genes piramidización de genes The fungus Pyricularia oryzae Cav., is the cause of the most important disease in rice cultivation, blast, due to its widespread presence in the world. Consequently, the development of rice plants tolerant to this disease is a key factor to maintain crop yields. By incorporating techniques such as marker-assisted selection (MAS) to screen genes of interest and the production of Double Haploid plants to generate homogeneous populations, we accelerate the development of resistant materials with several blast resistance genes. This study aims to obtain plants carrying five resistance genes to Pyricularia using molecular markers and tissue culture. Three parents were involved which have been identified as carriers of Pyricularia tolerance genes: CIFED 11 A (Pish, Pi2, and Piks-Zh), IRBLt-K59_LTH (Pi-t) and FL13032 (Pish and Pii). Two simple crosses were carried out (CIFED 11 A x IRBLt-K59 and CIFED 11 A x IRBLT-K59) and the F1 plants carrying the genes of each parent involved were selected, subsequently, A double cross was made between the two F1 to obtain a population of 1424 seeds that were planted to confirm the presence of the genes and with the selected plants the anther culture was carried out. In the tissue culture process, calluses were obtained, and plants were regenerated as a result of these. According to the phenotypic evaluation, double haploid plants were identified and evaluated to confirm the presence of the five resistance genes. Seven plants were confirmed as carriers of the genes of interest that present genotypic and phenotypic stability, which can be included in breeding program schemes for the generation of varieties tolerant to Pyricularia. 2024-06-12 2025-01-29T09:08:31Z 2025-01-29T09:08:31Z Poster https://hdl.handle.net/10568/170301 es Open Access application/pdf Pineda, M.; Agrono, T.; Quintero, C.; Morales, M.; Alvarez, M.F. (2024) Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave. Presentado en XIV Conferencia Internacional de Arroz para América Latina y el Caribe y la Novena Conferencia de Piricularia en Arroz y Trigo, 1-14 junio 2024. 1 p. |
| spellingShingle | rice arroz genes piramidización de genes Pineda, Marcela Agrono, Tomas Quintero, Constanza Morales, Mabel Alvarez, Maria Fernanda Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave |
| title | Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave |
| title_full | Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave |
| title_fullStr | Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave |
| title_full_unstemmed | Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave |
| title_short | Avances en el pre-mejoramiento del arroz: Estrategias de piramidación para mejorar la tolerancia a pyricularia (pyricularia oryzae cav.) Mediante la integración de cinco genes clave |
| title_sort | avances en el pre mejoramiento del arroz estrategias de piramidacion para mejorar la tolerancia a pyricularia pyricularia oryzae cav mediante la integracion de cinco genes clave |
| topic | rice arroz genes piramidización de genes |
| url | https://hdl.handle.net/10568/170301 |
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