Development of functional molecular markers for viviparous germination resistance in rice
Rice (Oryza sativa) plays a pivotal role in global food security. Understanding the genetics of rice cultivation is crucial, particularly for traits such as viviparous germination, which significantly influences germination and yield. Our research aimed to elucidate the genetic and molecular mechani...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/170024 |
| _version_ | 1855521111614160896 |
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| author | Lee, So-Myeong Kwon, Youngho Kim, Sung-Ryul Kang, Ju-Won Park, Hyeonjin Cha, Jin-Kyung Park, Dong-Soo Cho, Jun-Hyun Kim, Woojae Eom, Gyu-Hyeon Lee, Jong-Hee |
| author_browse | Cha, Jin-Kyung Cho, Jun-Hyun Eom, Gyu-Hyeon Kang, Ju-Won Kim, Sung-Ryul Kim, Woojae Kwon, Youngho Lee, Jong-Hee Lee, So-Myeong Park, Dong-Soo Park, Hyeonjin |
| author_facet | Lee, So-Myeong Kwon, Youngho Kim, Sung-Ryul Kang, Ju-Won Park, Hyeonjin Cha, Jin-Kyung Park, Dong-Soo Cho, Jun-Hyun Kim, Woojae Eom, Gyu-Hyeon Lee, Jong-Hee |
| author_sort | Lee, So-Myeong |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice (Oryza sativa) plays a pivotal role in global food security. Understanding the genetics of rice cultivation is crucial, particularly for traits such as viviparous germination, which significantly influences germination and yield. Our research aimed to elucidate the genetic and molecular mechanisms by which the Sdr4 gene influences viviparous germination and to develop novel molecular markers for this gene to enhance breeding strategies against viviparous germination. In all, 683 rice cultivars and 100 F2 plants were used for viviparous germination and genetic analysis using KASP (Kompetitive Allele-Specific PCR) and agarose gel-based markers related to viviparous germination tolerance. We developed and used a polymorphic agarose gel-based marker and a KASP marker targeting the Sdr4 gene. A genetic analysis of field-grown rice cultivars and the F2 population revealed that the two markers on Sdr4 were functional for the genomic selection of SNPs and InDels related to dormancy. The Pearson correlation coefficient (r = 0.74, p-value = 3.31 × 10−8) between the Sdr4-IND KASP marker genotype and viviparous germination rate demonstrated a significant positive correlation, supporting the marker’s utility for selecting rice varieties with diminished viviparous germination. This insight serves as a critical theoretical foundation for breeding strategies for developing early-maturing rice varieties with enhanced resistance to viviparous germination, addressing pivotal challenges in rice cultivation, and ensuring food security. |
| format | Journal Article |
| id | CGSpace170024 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1700242026-01-06T12:03:45Z Development of functional molecular markers for viviparous germination resistance in rice Lee, So-Myeong Kwon, Youngho Kim, Sung-Ryul Kang, Ju-Won Park, Hyeonjin Cha, Jin-Kyung Park, Dong-Soo Cho, Jun-Hyun Kim, Woojae Eom, Gyu-Hyeon Lee, Jong-Hee food security breeding programmes yields analysis of variance germination rice Rice (Oryza sativa) plays a pivotal role in global food security. Understanding the genetics of rice cultivation is crucial, particularly for traits such as viviparous germination, which significantly influences germination and yield. Our research aimed to elucidate the genetic and molecular mechanisms by which the Sdr4 gene influences viviparous germination and to develop novel molecular markers for this gene to enhance breeding strategies against viviparous germination. In all, 683 rice cultivars and 100 F2 plants were used for viviparous germination and genetic analysis using KASP (Kompetitive Allele-Specific PCR) and agarose gel-based markers related to viviparous germination tolerance. We developed and used a polymorphic agarose gel-based marker and a KASP marker targeting the Sdr4 gene. A genetic analysis of field-grown rice cultivars and the F2 population revealed that the two markers on Sdr4 were functional for the genomic selection of SNPs and InDels related to dormancy. The Pearson correlation coefficient (r = 0.74, p-value = 3.31 × 10−8) between the Sdr4-IND KASP marker genotype and viviparous germination rate demonstrated a significant positive correlation, supporting the marker’s utility for selecting rice varieties with diminished viviparous germination. This insight serves as a critical theoretical foundation for breeding strategies for developing early-maturing rice varieties with enhanced resistance to viviparous germination, addressing pivotal challenges in rice cultivation, and ensuring food security. 2024-12-04 2025-01-26T22:46:12Z 2025-01-26T22:46:12Z Journal Article https://hdl.handle.net/10568/170024 en Open Access MDPI Lee, So-Myeong, Youngho Kwon, Sung-Ryul Kim, Ju-Won Kang, Hyeonjin Park, Jin-Kyung Cha, Dong-Soo Park et al. "Development of Functional Molecular Markers for Viviparous Germination Resistance in Rice." Agronomy 14, no. 12 (2024): 2896. |
| spellingShingle | food security breeding programmes yields analysis of variance germination rice Lee, So-Myeong Kwon, Youngho Kim, Sung-Ryul Kang, Ju-Won Park, Hyeonjin Cha, Jin-Kyung Park, Dong-Soo Cho, Jun-Hyun Kim, Woojae Eom, Gyu-Hyeon Lee, Jong-Hee Development of functional molecular markers for viviparous germination resistance in rice |
| title | Development of functional molecular markers for viviparous germination resistance in rice |
| title_full | Development of functional molecular markers for viviparous germination resistance in rice |
| title_fullStr | Development of functional molecular markers for viviparous germination resistance in rice |
| title_full_unstemmed | Development of functional molecular markers for viviparous germination resistance in rice |
| title_short | Development of functional molecular markers for viviparous germination resistance in rice |
| title_sort | development of functional molecular markers for viviparous germination resistance in rice |
| topic | food security breeding programmes yields analysis of variance germination rice |
| url | https://hdl.handle.net/10568/170024 |
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