Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics
Recent research has shown that optimizing photosynthetic and stomatal traits holds promise for improved crop performance. However, standard phenotyping tools such as gas exchange systems have limited throughput. In this work, a novel approach based on a bespoke gas exchange chamber allowing combined...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/169942 |
| _version_ | 1855517412715134976 |
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| author | Faralli, Michele Mellers, Greg Wall, Shellie Vialet-Chabrand, Silvere Forget, Guillaume Galle, Alexander Van Rie, Jeron Gardner, Keith A. Ober, Eric S. Cockram, James Lawson, Tracy |
| author_browse | Cockram, James Faralli, Michele Forget, Guillaume Galle, Alexander Gardner, Keith A. Lawson, Tracy Mellers, Greg Ober, Eric S. Van Rie, Jeron Vialet-Chabrand, Silvere Wall, Shellie |
| author_facet | Faralli, Michele Mellers, Greg Wall, Shellie Vialet-Chabrand, Silvere Forget, Guillaume Galle, Alexander Van Rie, Jeron Gardner, Keith A. Ober, Eric S. Cockram, James Lawson, Tracy |
| author_sort | Faralli, Michele |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Recent research has shown that optimizing photosynthetic and stomatal traits holds promise for improved crop performance. However, standard phenotyping tools such as gas exchange systems have limited throughput. In this work, a novel approach based on a bespoke gas exchange chamber allowing combined measurement of the quantum yield of PSII (Fq'/Fm'), with an estimation of stomatal conductance via thermal imaging was used to phenotype a range of bread wheat (Triticum aestivum L.) genotypes. Using the dual-imaging methods and traditional approaches, we found broad and significant variation in key traits, including photosynthetic CO2 uptake at saturating light and ambient CO2 concentration (Asat), photosynthetic CO2 uptake at saturating light and elevated CO2 concentration (Amax), the maximum velocity of Rubisco for carboxylation (Vcmax), time for stomatal opening (Ki), and leaf evaporative cooling. Anatomical analysis revealed significant variation in flag leaf adaxial stomatal density. Associations between traits highlighted significant relationships between leaf evaporative cooling, leaf stomatal conductance, and Fq'/Fm', highlighting the importance of stomatal conductance and stomatal rapidity in maintaining optimal leaf temperature for photosynthesis in wheat. Additionally, gsmin and gsmax were positively associated, indicating that potential combinations of preferable traits (i.e. inherently high gsmax, low Ki, and maintained leaf evaporative cooling) are present in wheat. This work highlights the effectiveness of thermal imaging in screening dynamic gs in a panel of wheat genotypes. The wide phenotypic variation observed suggested the presence of exploitable genetic variability in bread wheat for dynamic stomatal conductance traits and photosynthetic capacity for targeted optimization within future breeding programmes. |
| format | Journal Article |
| id | CGSpace169942 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1699422025-10-26T12:53:20Z Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics Faralli, Michele Mellers, Greg Wall, Shellie Vialet-Chabrand, Silvere Forget, Guillaume Galle, Alexander Van Rie, Jeron Gardner, Keith A. Ober, Eric S. Cockram, James Lawson, Tracy kinetics photosynthesis stomatal conductance infrared imagery water use wheat Recent research has shown that optimizing photosynthetic and stomatal traits holds promise for improved crop performance. However, standard phenotyping tools such as gas exchange systems have limited throughput. In this work, a novel approach based on a bespoke gas exchange chamber allowing combined measurement of the quantum yield of PSII (Fq'/Fm'), with an estimation of stomatal conductance via thermal imaging was used to phenotype a range of bread wheat (Triticum aestivum L.) genotypes. Using the dual-imaging methods and traditional approaches, we found broad and significant variation in key traits, including photosynthetic CO2 uptake at saturating light and ambient CO2 concentration (Asat), photosynthetic CO2 uptake at saturating light and elevated CO2 concentration (Amax), the maximum velocity of Rubisco for carboxylation (Vcmax), time for stomatal opening (Ki), and leaf evaporative cooling. Anatomical analysis revealed significant variation in flag leaf adaxial stomatal density. Associations between traits highlighted significant relationships between leaf evaporative cooling, leaf stomatal conductance, and Fq'/Fm', highlighting the importance of stomatal conductance and stomatal rapidity in maintaining optimal leaf temperature for photosynthesis in wheat. Additionally, gsmin and gsmax were positively associated, indicating that potential combinations of preferable traits (i.e. inherently high gsmax, low Ki, and maintained leaf evaporative cooling) are present in wheat. This work highlights the effectiveness of thermal imaging in screening dynamic gs in a panel of wheat genotypes. The wide phenotypic variation observed suggested the presence of exploitable genetic variability in bread wheat for dynamic stomatal conductance traits and photosynthetic capacity for targeted optimization within future breeding programmes. 2024-11-15 2025-01-26T00:25:07Z 2025-01-26T00:25:07Z Journal Article https://hdl.handle.net/10568/169942 en Open Access application/pdf Oxford University Press Faralli, M., Mellers, G., Wall, S., Vialet-Chabrand, S., Forget, G., Galle, A., Van Rie, J., Gardner, K. A., Ober, E. S., Cockram, J., & Lawson, T. (2024). Exploring natural genetic diversity in a bread wheat multi-founder population: Dual imaging of photosynthesis and stomatal kinetics. Journal of Experimental Botany, 75(21), 6733-6747. https://doi.org/10.1093/jxb/erae233 |
| spellingShingle | kinetics photosynthesis stomatal conductance infrared imagery water use wheat Faralli, Michele Mellers, Greg Wall, Shellie Vialet-Chabrand, Silvere Forget, Guillaume Galle, Alexander Van Rie, Jeron Gardner, Keith A. Ober, Eric S. Cockram, James Lawson, Tracy Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics |
| title | Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics |
| title_full | Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics |
| title_fullStr | Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics |
| title_full_unstemmed | Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics |
| title_short | Exploring natural genetic diversity in a bread wheat multi-founder population: dual imaging of photosynthesis and stomatal kinetics |
| title_sort | exploring natural genetic diversity in a bread wheat multi founder population dual imaging of photosynthesis and stomatal kinetics |
| topic | kinetics photosynthesis stomatal conductance infrared imagery water use wheat |
| url | https://hdl.handle.net/10568/169942 |
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